Cultivation of uncultured marine microorganisms

Wang, Fengping; Li, Meng; Huang, Li; Zhang, Xiaohua

doi:10.1007/s42995-021-00093-z

Cited by 34 publications

(22 citation statements)

References 42 publications

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“…Inherently, culture-based approaches retrieve only a small fraction of the total bacterial diversity within any given environment, a phenomenon known as the “great plate anomaly” ( 20 – 22 ). Often however, it is not a case of being “unculturable” but of not yet knowing the (range of) conditions needed to culture specific microorganisms ( 23 ). Cultivating host-associated microorganisms can be challenging, as their nutrient requirements and cross-feeding networks are often unknown ( 24 ).…”

Section: Introductionmentioning

confidence: 99%

Insights into the Cultured Bacterial Fraction of Corals

et al. 2021

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Section: Introductionmentioning

confidence: 99%

Insights into the Cultured Bacterial Fraction of Corals

et al. 2021

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“…The reason may include the followings: (1) most of the pathogenic bacteria of seaweeds are opportunistic pathogenic bacteria which exhibit pathogenic virulence only under certain conditions (Wang et al, 2014). ( 2) the pathogenic bacteria of S. japonica can rarely be successful cultured in vitro, most of them are unculturable (Zhang et al, 2020;Wang et al, 2021; Prof. Gaoge Wang's pers. communication).…”

Section: Discussionmentioning

confidence: 99%

Pseudoalteromonas piscicida X-8 causes bleaching disease in farmed Saccharina japonica

et al. 2022

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“…The 21st century assisted to the rebirth of culture-dependent strategies to explore microbial diversity [ 19 , 21 , 95 , 96 , 97 , 98 ]. Study of the human microbiota is an excellent example of the potential and success of high-throughput culture techniques [ 87 , 99 ].…”

Section: Assessing Not-yet-cultured Biocatalystsmentioning

confidence: 99%

Marine Bioprospecting, Biocatalysis and Process Development

Rodrigues

Carvalho

2022

Microorganisms

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Oceans possess tremendous diversity in microbial life. The enzymatic machinery that marine bacteria present is the result of extensive evolution to assist cell survival under the harsh and continuously changing conditions found in the marine environment. Several bacterial cells and enzymes are already used at an industrial scale, but novel biocatalysts are still needed for sustainable industrial applications, with benefits for both public health and the environment. Metagenomic techniques have enabled the discovery of novel biocatalysts, biosynthetic pathways, and microbial identification without their cultivation. However, a key stage for application of novel biocatalysts is the need for rapid evaluation of the feasibility of the bioprocess. Cultivation of not-yet-cultured bacteria is challenging and requires new methodologies to enable growth of the bacteria present in collected environmental samples, but, once a bacterium is isolated, its enzyme activities are easily measured. High-throughput screening techniques have also been used successfully, and innovative in vitro screening platforms to rapidly identify relevant enzymatic activities continue to improve. Small-scale approaches and process integration could improve the study and development of new bioprocesses to produce commercially interesting products. In this work, the latest studies related to i. the growth of marine bacteria under laboratorial conditions, ii. screening techniques for bioprospecting, and iii. bioprocess development using microreactors and miniaturized systems are reviewed and discussed.

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Cultivation of uncultured marine microorganisms

Cited by 34 publications

References 42 publications

Insights into the Cultured Bacterial Fraction of Corals

Insights into the Cultured Bacterial Fraction of Corals

Pseudoalteromonas piscicida X-8 causes bleaching disease in farmed Saccharina japonica

Marine Bioprospecting, Biocatalysis and Process Development

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