Cultivation Strategy for Freshwater Macro- and Micro-Algae as Biomass Stock for Lipid Production

Abstract

Abstract. In this research, an algae cultivation strategy was studied. Integrating algae cultivation with wastewater treatment is currently seen as one of the most economical ways of producing algae biomass. A combination of an anaerobic baffled reactor (ABR) and a constructed wetland (CW) was applied for treating domestic wastewater with an additional collection tank for improving effluent quality. The effluent produced from the three stages was used as algae cultivation media and suplemented with 10% bold basal medium (BBM). The results showed both micro-and macro-algae growth and their lipid contents were higher when they were grown in effluent-BBM (9:1 v/v) media. The lipid content of the micro-algae mixed culture was 16.5% while for macro-algae Oedogonium sp and Cladophora sp it was 6.90% and 6.75% respectively.

Active and phenolic compounds in Spirogyra sp. PDNA1 is an antibiotic for some bacteria and fungi

“…The dry mass of the alga under study was used and crushed using a small ceramic mortar and an electric grinder. The dried alga powder was placed in a Whatman type 1 filter paper and arranged in such a way as to be a cellulose thimble (Soxhlet) [11]. Then, the transferring of the extract was done to the round flask (after emptying the separation column) of the rotary evaporator to reduce the volume of the solvent and reuse it again and transfer the resulting extract to a small airtight container for use in measurements and determination of the active compounds in it [12].…”

“…The phenols showed the highest inhibition rate of the phenolic-methanolic extract against (Klebsiella pneumoniae) (30) mm and the least inhibition against (Escherichia. coli) (11) mm while the phenolic hexane extract has the highest inhibition to (Salmonella typhi) (27) mm and the lowest effect was against (Escherichia coli) (10) mm. The antifungal effect of the phenolic methanolic extract recorded the highest percentage against (Candida albicans) (30) mm and had the lowest effect on Mucor racemosus (18) mm, while the phenolic hexane extract had the highest effect with Candida albicans (22.5) mm, and the least inhibition percentage was in Mucor racemosus with (11) mm.…”

“…coli) (11) mm while the phenolic hexane extract has the highest inhibition to (Salmonella typhi) (27) mm and the lowest effect was against (Escherichia coli) (10) mm. The antifungal effect of the phenolic methanolic extract recorded the highest percentage against (Candida albicans) (30) mm and had the lowest effect on Mucor racemosus (18) mm, while the phenolic hexane extract had the highest effect with Candida albicans (22.5) mm, and the least inhibition percentage was in Mucor racemosus with (11) mm. Therefore, the study aimed to isolate and identify the effective compounds of the methanolic and hexanoic extract of this algae, and active phenolic compounds were detected using GC-MS and HPLC technology.…”

Separation and Identification of a Number of Unsaturated Fatty Acids From Different Types of Algae

“…Dry moss mass was used, after crushing with a small ceramic mortar, the dried moss powder was placed in a Whatman 1 filter paper and formatted in such a way as to form a cellulose thimble (Soxhelet) [20].…”