Cultivo &lt;I&gt;in vitro&lt;/I&gt; de embriões zigóticos de &lt;I&gt;Butia eriospatha&lt;/I&gt; (Mart. ex Drude) Becc. DOI:10.5007/2178-4574.2011n40p70

Minardi, Bruno Degaspari; Voytena, Ana Paula Lorenzen; Randi, Áurea Maria; Zaffari, Gilmar Roberto

doi:10.5007/2178-4574.2011n40p70

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…For example, some authors used one (e.g., Buthia Eriospatha Mart. by [19]), others four (e.g., Jatropha sp. L. by [20]), and others eight embryos (e.g., Phoenix dactylifera L. by [21]) as samples for experiments on embryonic development.…”

Section: Introductionmentioning

confidence: 99%

Understanding In Vitro Embryo Development through Classical Germination Measurements: A Case Study of Dragon’s Blood (Croton lechleri Müll Arg.)

de Albuquerque,

Ribeiro-Oliveira,

de Oliveira-Júnior

et al. 2023

Agriculture

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Sample size fluctuation and the restriction of measurements that demonstrate kinetics (typical of physiological processes) are two of the largest inferential constraints in studies on embryonic development in vitro. Thus, we hypothesize that a practical and robust way of aggregating knowledge on aspects of embryonic development in vitro is to use measurements based on the binary counting component. These are typically used to measure the germination process (intraeminal embryonal development). Our biological model was Dragon’s blood (Croton lechleri Müll Arg.), a species native to the Amazon with great socioeconomic impact. Matrices originating from two populations (one native and another cultivated) were the source of biological material. From this material, we studied five sampling densities (5, 25, 50, and 100 embryos), forming a 2 × 4 factorial ANOVA. Among the measurements studied, the coefficient of variation of time, uncertainty, and the synchronization index were the most sensitive to sample-size fluctuation. The synchronization index, however, also proved to be an interesting measurement to detect the parental effect related to the place of occurrence of the matrices. The embryonic development ability, mean development time, and mean development rate were not affected by fluctuations in the sample size or the origin of the material, demonstrating highly conserved traits of the species. Finally, in general, the measurements based on binary counting demonstrated robustness for modeling embryonic growth.

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Section: Introductionmentioning

confidence: 99%

Understanding In Vitro Embryo Development through Classical Germination Measurements: A Case Study of Dragon’s Blood (Croton lechleri Müll Arg.)

de Albuquerque,

Ribeiro-Oliveira,

de Oliveira-Júnior

et al. 2023

Agriculture

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“…On top on that plants are only regenerate by seeds and, because of the dormancy they can take up to a year to germinate only around 50% and this is a concerning limitation about seedling establishment and species conservation (Broschat 1998;Fior et al 2011;Lopes et al 2011;Magalhães et al 2013;Schlindwein et al 2013). Many article reported the worrying genetic situation of the B. eriospatha species (Nazareno et al 2011; Nazareno and Dos Reis 2012, 2014b, a) and in vitro culture protocols have been studied to preserve its genetic diversity (Minardi et al 2011;Waldow et al 2013). Nonetheless, the knowledge regarding its reproductive biology is still limited, especially related to seed germination and desiccation tolerance physiology, which is essential for species conservation and restoration.…”

Section: Introductionmentioning

confidence: 99%

Biotechnologies Tools for Germination and Preservation of Butia Eriospatha Embryos (Arecaceae)

Goeten

Elias

Silva

et al. 2022

Preprint

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Butia eriospatha is an endemic species of Brazilian Atlantic Forest and due to the anthropic intervention, it is in the vulnerable conservation status. In this species, plants are only regenerate by seeds which have dormancy and slow as well low germination. To cope with the concern limitation of seedling establishment we study B. eriospatha embryo desiccation tolerance threshold as well the physiological requirement for in vitro germination and cryopreservation. Fresh embryos and desiccated ones were in vitro germinated using a culture medium with hormones and antioxidants. The embryo desiccation tolerance threshold was 0.14 gH2O.gDW-1 with 93.33% of germination. During embryos desiccation was observed a significant increase in PUT, which resulted in the ratio decrease [(SPD+SPM). PUT-1] DW), but the SPD was the most abundant polyamine overall. Significant increase in POD and APX activity led us to suggest that they are the main enzymes involved in cellular protection during desiccation. An increase of amino acids content, especially glutamic acid (Glu), leucine (Leu), lysine (Lys), glutamine (Gln), which are known as osmoprotectors, also was observed. A specific embryo desiccation stage (0.14 gH2O gDW-1) associated to its biochemical state were successfully used in the cryopreservation protocol and result in more than 90% of recovery and in vitro germination. The physiological and biochemistry approach of this study associated to an applied protocol for plant genetic resources conservation of B. eriospatha embryo can be potentially used for other Arecaceae species.

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“…Entre os aspectos importantes na cultura de embriões estão a definição do meio de cultura, condições de luminosidade e temperatura, condições da planta matriz, a qualidade dos explantes, entre outros (MINARDI et al, 2011). Conforme Costa e Aloufa (2007) um dos aspectos mais importante da cultura de embriões, é a determinação do meio de cultura, pois este é que sustentará o crescimento dos embriões.…”

Section: Introductionunclassified

Superação in Vitro Da Dormência De Embriões De Pereira Oriundos De Hibridações

Lima

Rosa

Tomaz

et al. 2016

R. Eletr. Cient. da Uergs

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Resumo -Objetivou-se verificar a influência de diferentes meios de cultura na superação in vitro da dormência de embriões oriundos de hibridação. Embriões foram retirados de sementes oriundas de hibridações controladas e inoculados nos meios de cultivo: MS; MS/2; QL e QL/2, mantidos em sala de crescimento a 25±2ºC e fotoperíodo de 16 horas. O delineamento experimental foi inteiramente casualizado com dois fatores analisados (hibradações e meios de cultura), em esquema fatorial 3X4, sendo que cada repetição constituiu-se de um frasco, com cinco embriões cada. Aos 60 dias de cultivo, o material foi avaliado. Os dados foram submetidos à análise de variância e as médias dos tratamentos comparadas estatisticamente pelo teste de Tuckey (α=0,05). Houve interação entre os fatores (meios de cultura e hibridações) para todas as variáveis respostas analisadas. A maior percentagem de germinação (53,49%), foi obtida na associação do meio de cultura QL/2 com embriões oriundos do tratamento 2. O maior número e comprimento de brotações foi verificado na combinação de embriões do tratamento 1 cultivadas em meio QL e o maior crescimento radicular foi observado no cultivo realizado em meio MS com os embriões oriundos dos tratamentos 1 e 2. O meio QL/2 proporcionou o maior número de embriões de porta-enxertos de pereira germinados. Entretanto o meio QL completo é o mais adequado para multiplicação. Palavras-Chave -Porta-enxerto. Polinização aberta. Packham's Triumph. In vitro. Melhoramento vegetal.Abstract -This study aimed to investigate the influence of different culture media on the in vitro overcoming dormancy of embryos derived from hybridization. Embryos were removed from seeds from controlled hybridizations and inoculated in culture media: MS; MS /2; QL and QL /2, maintained in a growth room at 25±2°C and 16 h of photoperiod. The experimental design was completely randomized with two factors analyzed (hybridizations and culture media) in a factorial 3x4, each repetition consisted of a bottle with five embryos each. After 60 days of culture, the material was analyzed. The data were submitted to analysis of variance and treatment means compared statistically by the Tukey test (α = 0.05). There was interaction between the factors (culture media and hybridizations) for all variables. The highest percentage of germination (53.49%) was obtained from the association of culture medium QL / 2 with embryos from the 2 treatment. The greater number and length of sprouts was observed in the combination treatment of embryos cultured in medium 1 QL and root growth was observed in the cultivation carried out on MS medium with the embryos from treatments 1 and 2. The medium QL / 2 provided the greatest number of germinated pear rootstocks embryos. However, QL medium is suitable is the most suitable for multiplication.

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Cultivo <I>in vitro</I> de embriões zigóticos de <I>Butia eriospatha</I> (Mart. ex Drude) Becc. DOI:10.5007/2178-4574.2011n40p70

Abstract: permite uso não-comercial, distribuição e reprodução em qualquer meio, desde que este trabalho original seja devidamente citado. Cultivo in vitro de embriões zigóticos de Butia eriospatha (Mart. ex Drude) Becc.

Cited by 4 publications

References 6 publications

Understanding In Vitro Embryo Development through Classical Germination Measurements: A Case Study of Dragon’s Blood (Croton lechleri Müll Arg.)

Understanding In Vitro Embryo Development through Classical Germination Measurements: A Case Study of Dragon’s Blood (Croton lechleri Müll Arg.)

Biotechnologies Tools for Germination and Preservation of Butia Eriospatha Embryos (Arecaceae)

Superação in Vitro Da Dormência De Embriões De Pereira Oriundos De Hibridações

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