Cultivos celulares para el desarrollo de vacunas DNA frente a virus de peces utilizando el modelo de trucha arco iris / rhabdovirus de la septicemia hemorrágica vírica (VHSV)

Haro, Neila Álavarez de

doi:10.18002/10612/4549

Cited by 1 publication

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References 261 publications

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“…However, the immunodominant rickettsial antigens responsible for stimulating CD4+ and CD8+ cells have not yet been clearly identified and existing studies have been based on animal models. In recent years, ex vivo assays have been done using presenting cells (macrophages or DC) maintained in cultures with sensitized lymphocytes, and the determination of the cytokine response generated under this strategy has created high expectations, with high predictive value and a human profile, resulting in potential antigens to be used in the development of human vaccines [ 22 , 23 ]. The use of autologous systems has the advantage of matching the main HLA loci for cases in which antigenic recognition is required.…”

Section: Discussionmentioning

confidence: 99%

Rickettsia Vaccine Candidate pVAX1-OmpB24 Stimulates TCD4+INF-γ+ and TCD8+INF-γ+ Lymphocytes in Autologous Co-Culture of Human Cells

et al. 2023

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Background: In recent years, promising vaccination strategies against rickettsiosis have been described in experimental animal models and human cells. OmpB is considered an immunodominant antigen that is recognized by T and B cells. The aim of this study was to identify TCD4+INF-γ+ and TCD8+INF-γ+ lymphocytes in an autologous system with macrophages transfected with the vaccine candidate pVAX1-OmpB24. Lymphocytes and monocytes from 14 patients with Rickettsia were isolated from whole blood. Monocytes were differentiated into macrophages and transfected with the plasmid pVAX1-OmpB24 pVax1. Isolated lymphocytes were cultured with transfected macrophages. IFN-γ-producing TCD4+ and TCD8+ lymphocyte subpopulations were identified by flow cytometry, as was the percentage of macrophages expressing CD40+, CD80+, HLA-I and HLA-II. Also, we analyzed the exhausted condition of the T lymphocyte subpopulation by PD1 expression. Macrophages transfected with pVAX1-OmpB24 stimulated TCD4+INF-γ+ cells in healthy subjects and patients infected with R. typhi. Macrophages stimulated TCD8+INF-γ+ cells in healthy subjects and patients infected with R. rickettsii and R. felis. Cells from healthy donors stimulated with OmpB-24 showed a higher percentage of TCD4+PD1+. Cells from patients infected with R. rickettsii had a higher percentage of TCD8+PD-1+, and for those infected with R. typhi the larger number of cells corresponded to TCD4+PD1+. Human macrophages transfected with pVAX1-OmpB24 activated TCD4+IFN-γ+ and CD8+IFN-γ+ in patients infected with different Rickettsia species. However, PD1 expression played an important role in the inhibition of T lymphocytes with R. felis.

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Section: Discussionmentioning

confidence: 99%

Rickettsia Vaccine Candidate pVAX1-OmpB24 Stimulates TCD4+INF-γ+ and TCD8+INF-γ+ Lymphocytes in Autologous Co-Culture of Human Cells

et al. 2023

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Cultivos celulares para el desarrollo de vacunas DNA frente a virus de peces utilizando el modelo de trucha arco iris / rhabdovirus de la septicemia hemorrágica vírica (VHSV)

Abstract: Ahmadi N, A Oryan, M Akhlaghi, and A Hosseini. 2013. Tissue distribution of infectious pancreatic necrosis virus serotype Sp in naturally infected cultured rainbow trout, Oncorhynchus mykiss (Walbaum): an immunohistochemical and nested-PCR study.

Cited by 1 publication

References 261 publications

Rickettsia Vaccine Candidate pVAX1-OmpB24 Stimulates TCD4+INF-γ+ and TCD8+INF-γ+ Lymphocytes in Autologous Co-Culture of Human Cells

Rickettsia Vaccine Candidate pVAX1-OmpB24 Stimulates TCD4+INF-γ+ and TCD8+INF-γ+ Lymphocytes in Autologous Co-Culture of Human Cells

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