Culturable microbiota of ranched southern bluefin tuna (<i>Thunnus maccoyii </i>
Castelnau)

Valdenegro-Vega, Victoria A.; Naeem, Shahid; Carson, J; Bowman, John P.; Real, José Luis Tejedor del; Nowak, Barbara F.

doi:10.1111/jam.12286

Cited by 31 publications

(23 citation statements)

References 34 publications

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“…Absence of mortalities in our study may be attributed to the absence of pathogenic Vibrio and Photobacterium (Munday et al, 2003;Valdenegro-Vega et al, 2013). Similarly, Valdenegro-Vega et al (2013) failed to observe a relationship between isolated bacteria and moribund or dead fish.…”

Section: Discussionsupporting

confidence: 47%

“…In contrast to HPC, Vibrio count was higher on skin and gills than in sea water at both samplings. This suggests that sea water influences the bacterial community on the skin (Kapetanović et al, 2013a;Valdenegro-Vega et al, 2013), and that this influence is likely to be complex. Such influence may help explain why Gram-positive bacteria were so abundant on the tuna skin in our study.…”

Section: Discussionmentioning

confidence: 99%

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Culturable microbiota associated with farmed Atlantic bluefin tuna (Thunnusthynnus)

Kapetanović

Smrzlić

Valić

et al. 2017

Aquat. Living Resour.

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This study examined culturable microbiota associated with farming of Atlantic bluefin tuna (Thunnus thynnus), an economically significant aquacultured fish species, in autumn and late spring. While sea water gave higher heterotroph plate counts than tuna skin and gill swabs, the opposite was true for Vibrio plate counts. Most bacteria identified from skin and gill swabs at both samplings were Gram-negative and comprised Pasteurella and Moraxella genera. At both samplings, Moraxella was the most abundant genus in internal organs, followed by Pasteurella and Brevundimonas, which were present in fewer organs. While none of the microbiota identified is known to be pathogenic to tuna, several are spoilage bacteria: Klebsiella, Moraxella, Pseudomonas, Staphylococcus, Vibrio and Weissella. Total abundance of bacteria was greater on tuna skin than on gills. These results provide information about the bacterial community normally associated with healthy farmed Atlantic bluefin tuna, which will be helpful for assessing how changes in this community may affect farmed tuna health, risk of spoilage and safety of raw and processed tuna.

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Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 99%

Culturable microbiota associated with farmed Atlantic bluefin tuna (Thunnusthynnus)

Kapetanović

Smrzlić

Valić

et al. 2017

Aquat. Living Resour.

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“…It may be possible that bacteria found on whale skin also occur within the respiratory tract or epithelial cells. Tenacibaculum has been associated with the microbiome of other marine species such as southern bluefin tuna (Thunnus maccoyii castelnau) (Valdenegro-Vega et al, 2013), while Psychrobacter is part of the thresher shark and rainbow trout skin microbiome (Lowrey et al, 2015;Doane et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

An Economical Custom-Built Drone for Assessing Whale Health

Pirotta

Smith²,

Ostrowski

et al. 2017

Front. Mar. Sci.

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Drones or Unmanned Aerial Vehicles (UAVs) have huge potential to improve the safety and efficiency of sample collection from wild animals under logistically challenging circumstances. Here we present a method for surveying population health that uses UAVs to sample respiratory vapor, 'whale blow,' exhaled by free-swimming humpback whales (Megaptera novaeangliae), and coupled this with amplification and sequencing of respiratory tract microbiota. We developed a low-cost multirotor UAV incorporating a sterile petri dish with a remotely operated 'blow' to sample whale blow with minimal disturbance to the whales. This design addressed several sampling challenges: accessibility; safety; cost, and critically, minimized the collection of atmospheric and seawater microbiota and other potential sources of sample contamination. We collected 59 samples of blow from northward migrating humpback whales off Sydney, Australia and used high throughput sequencing of bacterial ribosomal gene markers to identify putative respiratory tract microbiota. Model-based comparisons with seawater and dronecaptured air demonstrated that our system minimized external sources of contamination and successfully captured sufficient material to identify whale blow-specific microbial taxa. Whale-specific taxa included species and genera previously associated with the respiratory tracts or oral cavities of mammals (e.g., Pseudomonas, Clostridia, Cardiobacterium), as well as species previously isolated from dolphin or killer whale blowholes (Corynebacteria, others). Many examples of exogenous marine species were identified, including Tenacibaculum and Psychrobacter spp. that have been associated with the skin microbiota of marine mammals and fish and may include pathogens. This information provides a baseline of respiratory tract microbiota profiles of contemporary whale health. Customized UAVs are a promising new tool for marine megafauna research and may have broad application in cost-effective monitoring and management of whale populations worldwide.

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“…Nevertheless, most studies have been limited either because of their focus on cultured fish species (Desai et al, 2012;Wu et al, 2013;Zarkasi et al, 2014Zarkasi et al, , 2016Schmidt et al, 2016;Dehler et al, 2017) or because of methodological approaches that offer limited taxonomical resolution (e.g. 16S rRNA amplicon sequencing (Star et al, 2013;Ye et al, 2014;Llewellyn et al, 2016;Riiser et al, 2018) or dependence on bacterial cultivation (Kim et al, 2007;Martin-Antonio et al, 2007;Valdenegro-Vega et al, 2013). Therefore, there remains a lack of detailed, baseline compositional data comparing healthy wild fish from the same species that live in different habitats with a variety of environmental conditions (Uchii et al, 2006;Egerton et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Switching on the light: using metagenomic shotgun sequencing to characterize the intestinal microbiome of Atlantic cod

Riiser

Haverkamp

Varadharajan

et al. 2019

Environmental Microbiology

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Summary Atlantic cod (Gadus morhua) is an ecologically important species with a wide‐spread distribution in the North Atlantic Ocean, yet little is known about the diversity of its intestinal microbiome in its natural habitat. No geographical differentiation in this microbiome was observed based on 16S rRNA amplicon analyses, yet such finding may result from an inherent lack of power of this method to resolve fine‐scaled biological complexity. Here, we use metagenomic shotgun sequencing to investigate the intestinal microbiome of 19 adult Atlantic cod individuals from two coastal populations in Norway–located 470 km apart. Resolving the species community to unprecedented resolution, we identify two abundant species, Photobacterium iliopiscarium and Photobacterium kishitanii, which comprise over 50% of the classified reads. Interestingly, the intestinal P. kishitanii strains have functionally intact lux genes, and its high abundance suggests that fish intestines form an important part of its ecological niche. These observations support a hypothesis that bioluminescence plays an ecological role in the marine food web. Despite our improved taxonomical resolution, we identify no geographical differences in bacterial community structure, indicating that the intestinal microbiome of these coastal cod is colonized by a limited number of closely related bacterial species with a broad geographical distribution.

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Culturable microbiota of ranched southern bluefin tuna (Thunnus maccoyii Castelnau)

Cited by 31 publications

References 34 publications

Culturable microbiota associated with farmed Atlantic bluefin tuna (Thunnusthynnus)

Culturable microbiota associated with farmed Atlantic bluefin tuna (Thunnusthynnus)

An Economical Custom-Built Drone for Assessing Whale Health

Switching on the light: using metagenomic shotgun sequencing to characterize the intestinal microbiome of Atlantic cod

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