Culturable Microorganisms Associated with Sea Cucumbers and Microbial Natural Products

Chen, Lei; Wang, Xiaoyu; Liu, Run-Ze; Wang, Guangyu

doi:10.3390/md19080461

Cited by 13 publications

(11 citation statements)

References 74 publications

(206 reference statements)

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“…Twenty-nine genera of fungi belonging to 24 families in the phylum Ascomycota have been cultured from five genera of sea cucumbers: Holothuria , Cucumaria , Stichopus , Apostichopus , and Eupentacta . The dominant fungal genus was found to be Aspergillus , followed by Penicillium (reviewed by [ 5 ]). Fungal viruses, or mycoviruses, have been widely detected in all the major fungal phyla, and usually cause asymptomatic infections.…”

Section: Introductionmentioning

confidence: 99%

“…To date, 145 natural products have been isolated from microorganisms associated with sea cucumbers. These compounds include alkaloids, polyketides and terpenoids (reviewed by [ 5 ]). Exploiting microorganisms as metabolite and enzyme producers is very advantageous because a fungus can be cultivated in vitro , allowing further studies to be conducted without environmental impact.…”

Section: Introductionmentioning

confidence: 99%

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Diversity and characterization of culturable fungi associated with the marine sea cucumber Holothuria scabra

Wingfield,

Atcharawiriyakul,

Jitprasitporn

2024

PLoS ONE

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Fungi associated with the marine echinoderm, Holothuria scabra, produces extracellular enzymes and bioactive metabolites, and mycoviruses that could be used for biotechnological and pharmaceutical applications. The species identification based on molecular and morphological characteristics classified the culturable fungi into twenty-three genera belonging to eight orders, Chaetothyriales, Eurotiales, Hypocreales, Mucorales, Mycosphaerellales, Onygenales, Pleosporales and Venturiales, from four classes, Eurotiomycetes, Dothideomycetes, Mucoromycetes and Sordariomycetes of the two phyla Ascomycota and Mucoromycota. The most frequent genera were Aspergillus (relative frequency, 45.30%) and Penicillium (relative frequency, 22.68%). The Menhinick species richness and Shannon species diversity indices were 1.64 and 2.36, respectively, indicating a high diversity of fungi. An enzymatic production test revealed that sixteen isolates could produce proteases and amylases at different levels. The presence of mycoviruses was detected in eight isolates with different genomic profiles. Thirty-two of the 55 isolates produced antimicrobial metabolites which had an inhibitory effect on various microbial pathogens. Most of these active isolates were identified as Aspergillus, Penicillium and Trichoderma. Notably, Aspergillus terreus F10M7, Trichoderma harzianum F31M4 and T. harzianum F31M5 showed the most potent activity against both Gram-positive and Gram-negative bacteria and human pathogenic fungi. Our study represents the first report of the mycobiota associated with the marine echinoderm Holothuria scabra.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diversity and characterization of culturable fungi associated with the marine sea cucumber Holothuria scabra

Wingfield,

Atcharawiriyakul,

Jitprasitporn

2024

PLoS ONE

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“…Natural products refer to components or metabolites from animals, plants, insects, and microorganisms, such as proteins, peptides, polysaccharides, and alkaloids [16][17][18]. Natural products have been used as medicines for thousands of years.…”

Section: Introductionmentioning

confidence: 99%

A Brief Review of Natural Products with Urate Transporter 1 Inhibition for the Treatment of Hyperuricemia

Yuan

Cheng

Sheng

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Hyperuricemia is a common disease caused by a high level of uric acid. Urate transporter 1 (URAT1) is an important protein and mediates approximately 90% of uric acid reabsorption. Therefore, the URAT1 inhibitor is a class of uricosuric medicines widely used in the clinic for the treatment of hyperuricemia. To find the new medicine with stronger URAT1 inhibition and lower toxicity, researchers have been exploring natural products. This study systematically summarizes the natural products with URAT1 inhibition. The results show that many natural products are potential URAT1 inhibitors, such as flavonoids, terpenoids, alkaloids, coumarins, stilbenes, and steroids, among which flavonoids are the most promising source of URAT1 inhibitors. It is worth noting that most studies have focused on finding natural products with inhibition of URAT1 and have not explored their activities and mechanisms toward URAT1. By reviewing the few existing studies of the structure-activity relationship and analyzing common features of natural products with URAT1 inhibition, we speculate that the rigid ring structure and negative charge may be the keys for natural products to produce URAT1 inhibition. In conclusion, natural products are potential URAT1 inhibitors, and exploring the mechanism of action and structure-activity relationship will be an important research direction in the future.

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“…12 Sea cucumber is a bioactive natural product, which is a nutrientrich, invertebrate found in the deep sea. 13 Sea cucumber contains many distinctive components such as methyltetradecanoic acid, triterpene glycoside compounds, chondroitin sulfates, glycosaminoglycan, sulfated polysaccharides, vitamins, minerals, and lectins and these components possess many bioactive metabolites that produce antimicrobial, anti-oxidant, anti-angiogenic, anti-inflammatory, immunomodulatory, and anticancer properties. [14][15][16][17] Frondoside A (FA) is a triterpenoid monosulfated glycoside, isolated from commercially harvested edible sea cucumber Cucumaria frondosa.…”

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confidence: 99%

Effect of intra‐alveolar delivery of Frondoside A on inflammatory response of delayed tooth replantation

Herr,

Ye,

Kang

et al. 2023

Dental Traumatology

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Background/AimFrondoside A is a sea cucumber extract which is well known for its anti‐inflammatory and immunomodulatory properties. The purpose of this study was to evaluate the effect of Frondoside A application in the alveolar socket on inflammatory responses after delayed replantation in rat teeth.Materials and MethodsHuman periodontal ligament cells were cultured and exposed to Frondoside A. Cell‐counting kit‐8 assay was performed to evaluate the cell viability and nitric oxide assay was performed to assess the anti‐inflammatory effect of Frondoside A. Molars were extracted from 32 Sprague–Dawley rats and randomly divided into control and Frondoside A groups. After 30 min of extra‐oral dry time, molars were replanted. In the Frondoside A group, Frondoside A solution was applied in the alveolar socket before replantation. The animals were sacrificed after 28 days and histologically and immunohistochemically evaluated.Results0.5 μM Frondoside A showed higher cellular viability at 6 h and lower production of nitric oxide compared with other Frondoside A solutions (p < .05). The Frondoside A group demonstrated lower inflammatory resorption scores in both middle 1/3 and apical 1/3 of root compared to the control group (p < .05). The Frondoside A group showed lower levels of expression in both cathepsin K and CD45 compared with the control group (p < .05).ConclusionsWithin the limits of this study, intra‐alveolar delivery of Frondoside A alleviates inflammatory root resorption in delayed replantation of rat teeth.

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Culturable Microorganisms Associated with Sea Cucumbers and Microbial Natural Products

Cited by 13 publications

References 74 publications

Diversity and characterization of culturable fungi associated with the marine sea cucumber Holothuria scabra

Diversity and characterization of culturable fungi associated with the marine sea cucumber Holothuria scabra

A Brief Review of Natural Products with Urate Transporter 1 Inhibition for the Treatment of Hyperuricemia

Effect of intra‐alveolar delivery of Frondoside A on inflammatory response of delayed tooth replantation

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