Cultural and morphological characterization of rhizospheric isolates of fungal antagonist Trichoderma

Abstract

Abstract:The genus Trichoderma contains species that are of a great economic importance due to their ability to act as biological control agents against a large variety of fungal plant pathogens. In the present investigation thirty isolates of the Trichoderma sp. were obtained from the rhizosphere soils of different plants at different locations at Nainital, Almora, Udham Singh Nagar, Derhadun, Haridwar and Tehri Garhwal districts of Uttarakhand (India). The isolates were characterized on the basis of their cultural and morphological characteristics. The cultural characteristics included linear growth, colony colour, pigmentation and growth pattern. Morphological characteristics studied were structure, shape and arrangement of conidiophores, phialides and conidia. Out of thirty isolates, 6 isolates namely PB10, PB13, PB23, PB26, PB27 and PB28 were identified as T. virens and remaining 24 isolates as T. harzianum.

Introductory Chapter: Identification and Isolation of Trichoderma spp. - Their Significance in Agriculture, Human Health, Industrial and Environmental Application

“…Green colony pigmentation after incubation for 7 days at 28°C on potato dextrose agar (PDA) was observed in Trichoderma cultures isolated from soil samples in Kliran [5]. Rhizospheric isolates revealed pale or yellowish color of reverse colonies at 25 and 30°C with rapid growth, loosely arranged conidia, and effused conidiation [13]. An ellipsoidal, obovoid, and bowling pin phialides were observed in Trichoderma spp.…”

Evaluation of native Trichoderma isolates for the management of sugarcane smut (Ustilago scitaminea) in sugar plantations of Ethiopia

“…The experiment was repeated twice. The growth rate of Trichoderma isolates in dual culture was determined based on the radial growth of the isolates in two consecutive measurements following the formula used by Sharma and Singh (2014) and Sekhar et al (2017): GR ¼ Rs À Rf =As À Af ; where GR = growth rate of the isolate in cm day −1 ; Rs = radial growth in the second measurement, Rf = radial growth in the first measurement, As = age of the colony at second measurement in days and Af = age of the colony at the first measurement in days.…”

In vitro ASSESSMENT OF Trichoderma asperellum ISOLATED FROM PLANT RHIZOSPHERE AND EVALUATION OF THEIR POTENTIAL ACTIVITY AGAINST SOME PATHOGENIC FUNGI

“…Morphological characterizations, including (mycelial color, colony texture and shape) and microscopic observations, including (conidia shape, conidia color, conidiophore -branching, phialide width and phialide length) were conducted according to Sharma and Singh (2014). Considering all the morphological characters, isolates of Trichoderma were placed under suitable group according to an interactive key provided by Samuels et al (2002) at http://nt.arsgrin.gov/taxadescriptions/keys/ FrameKey.cfm?…”