Cultural, Morphological and Biochemical Variability Studies among the Isolates of Alternaria solani, the Causal Agent of Early Blight Disease of Tomato

Nagesh,; Mushrif, S. K.; Sangeetha, C. G.; Reddy, T. Bhupal; Kumar, Jitender

doi:10.23910/1.2021.2491

Cited by 4 publications

(1 citation statement)

References 25 publications

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“…The study of variability plays a crucial role in documenting shifts within populations and individuals, as an indicator of the presence of distinct pathotypes (Nagesh et al, 2021). Although A. brassicola reproduces asexually, the isolates of this pathogen causing leaf blight disease in cabbage show significant variability in morphology, culture and genetic composition.…”

Section: Introductionmentioning

confidence: 99%

Variability of Alternaria brassicicola Causing Leaf Blight of Cabbage in Major Cabbage Growing Districts of Assam, India

Hazowary,

Upamanya,

Kashyap

2023

IJBSM

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The research was conducted at Assam Agricultural University, Jorhat, Assam, India during September, 2021–May, 2022 to study the variation of Alternaria brassiciola infecting cabbage. Fifteen isolates of Alternaria brassicicola which infected cabbage were collected from six different districts in Assam and their variability was investigated. Within the in vitro setting, it was observed that all isolates exhibited significant variation in terms of conidial length, breadth, and septal count. The isolates displayed a range of average conidial length and breadth spanning from 20.45µm to 29.74µm and 6.56µm to 9.89µm, respectively. Additionally, the isolates exhibited a variation in transverse septa with counts ranging from 4 to 6 and longitudinal septa, ranging from 0.13 to 0.41. Regarding the colony colour, four distinct variations, ranging from dark grey-green to dark brown-green and from mild olive green to greyish olive green were observed. These colonies featured edges in shades of white, greyish, brownish, or green edges and their shapes varied between round and irregular, accompanied by either a fluffy or compressed growth pattern. The diameter of the radial growth, observed on the fifteenth day after inoculation, varied from 57.3 mm to 84.2 mm. In RAPD analysis it was evident that the Jorhat isolates (AbJor_2) and the Barpeta isolate, (AbBa_1) exhibited the highest genetic similarity, as indicated by the lowest genetic distance with the coefficient of similarity 0.484. Conversely, the Jorhat isolates (AbJor_3) and Barpeta isolate (AbBa_1) shared the least genetic similarity, with the coefficient of similarity 0.021.

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Section: Introductionmentioning

confidence: 99%

Variability of Alternaria brassicicola Causing Leaf Blight of Cabbage in Major Cabbage Growing Districts of Assam, India

Hazowary,

Upamanya,

Kashyap

2023

IJBSM

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Detection of Alternaria solani with high accuracy and sensitivity during the latent period of potato early blight

et al. 2022

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Early blight (EB) disease, caused mainly by Alternaria solani, is an economic threat to potato and tomato production worldwide. Thus, accurate and sensitive detection of the fungal pathogen of this disease in plants at the early infection stage is important for forecasting EB epidemics. In this study, we developed an RNA-based method that enables highly accurate and sensitive A. solani detection in a whole potato leaf at a single spore level based on quantitative real-time polymerase chain reaction (qPCR). We discovered jg1677, a highly expressed gene whose full-length coding sequence is very specific for A. solani, by analyzing A. solani transcripts isolated from enhanced high throughput transcriptome of infected potato leaves by A. solani and using the National Center for Biotechnology Information’s basic local alignment search tool. The specificity of the primers derived from jg1677 was determined using 22 isolates of common potato pathogens, including seven Alternaria isolates. Detecting jg1677 transcripts with qPCR is 1,295 times more sensitive than detecting genomic DNA. In addition, the expression pattern of jg1677 at different infection stages was determined by qPCR. What is more, jg1677 was expressed relatively stable between 15 and 35°C in infected leaves, and its expression was virtually unaffected in isolated leaves left at room temperature for 24 h. Our work provides a much more sensitive and accurate method compared to conditional DNA-based ones, permitting a very early diagnosis of EB and lowering the risk of EB epidemics.

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Editorial: Perspective challenges for applied research in potato pathogens: From molecular biology to bioinformatics

Lahlali

Gachara²,

Özer³

et al. 2023

Front. Microbiol.

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Cultural, Morphological and Biochemical Variability Studies among the Isolates of Alternaria solani, the Causal Agent of Early Blight Disease of Tomato

Cited by 4 publications

References 25 publications

Variability of Alternaria brassicicola Causing Leaf Blight of Cabbage in Major Cabbage Growing Districts of Assam, India

Variability of Alternaria brassicicola Causing Leaf Blight of Cabbage in Major Cabbage Growing Districts of Assam, India

Detection of Alternaria solani with high accuracy and sensitivity during the latent period of potato early blight

Editorial: Perspective challenges for applied research in potato pathogens: From molecular biology to bioinformatics

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