Cultural, Morphological and Molecular Characterization  of Fusarium verticillioides causing Maize Ear Rot under Punjab Condition

Kaur, Kiranjot; Kaur, Jaspal; Puyam, Anita; Singh, K.P.

doi:10.20546/ijcmas.2020.910.205

Cited by 2 publications

(2 citation statements)

References 10 publications

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“…The ability of TSA32-1 to suppress the disease caused by F. graminearum and P. ulimum on corn and pepper was evaluated in pot tests with autoclaved soil [ 20 , 23 ]. The corn and pepper seeds, either coated or uncoated with TSA32-1 were sowed into the sterilized soil (50 g) and placed in a plastic pot with a diameter of approximately 60 mm and a height of 65 mm.…”

Section: Methodsmentioning

confidence: 99%

Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi

Kim

Song²,

Kim³

et al. 2022

JoF

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The use of synthetic fungicides has caused major problems such as soil and water pollution and negatively affects non-target species. Microbial biocontrol agents are needed for crop disease management to reduce agrochemical use. Bacillus and related genera produce secondary metabolites with agricultural applications, such as the pathogen-control agent Bacillus velezensis. We isolated B. velezensis TSA32-1 from soil and identified its characteristics by sequencing its 16S rRNA. B. velezensis TSA32-1 showed enzyme activity and antimicrobial effects against phytopathogenic fungi by inhibiting the growth of Fusarium graminearum, F. fujikuroi, Alternatia alternate, and Diaporthe actinidiae. Additionally, B. velezensis TSA32-1 protected diseases in corn and pepper seeds caused by F. graminearum and Pythium ultimum. The complete genome of B. velezensis TSA32-1 was 4.05 Mb with a G+C content of 46.3 mol % and possessed the bacillaene biosynthesis cluster, a polyketide that inhibits protein biosynthesis. We also detected a surfactin synthesis cluster, known as non-ribosomal peptide synthetases, which biosynthesizes the antibacterial substance lipopeptide. Surfactin, and fengycin family compounds, secondary metabolites known as key factors in biological control, also detected B. velezensis TSA32-1 which shows potential as a biocontrol agent for controlling plant pathogens in agriculture.

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Section: Methodsmentioning

confidence: 99%

Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi

Kim

Song²,

Kim³

et al. 2022

JoF

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“…for F. verticillioides to obtain 179 bp, 585 bp, and 800 bp ampli cation products respectively(Amatulli et al 2012;Kaur et al 2020;Deepa et al 2018). …”

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confidence: 99%

Seed coating with novel sodium alumino silicate margosa composite – A proficient technique to reduce mycotoxins of Fusarium fujikuroi species complex inciting discoloration of rice

SINGH,

Sharma,

Mandal

et al. 2024

Preprint

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Infestation of fungi during storage of grains causes about 4.6–6% wastage of cereal grains. Among other fungi Fusarium fujikuroi speciescomplex (FFSC) is one of the major causes of grain discoloration of rice. Due to the negative impacts of toxicgenic FFSC complex on seed health and quality metrics, it significantly reduces the market value of rice seeds. Apart from nutrient losses to rice seeds toxic fungal metabolites like fumonisin B1, fumonisin B2, fusaric acid, and gibberellic acid produced by FFSC complex interacts with physiological parameters influencing the integrity of seeds during storage. Due to the detrimental effects on seed germination and vitality, chemical seed treatments are usually avoided while the seeds are in storage. This paper presents the efficacy of novel sodium alumino silicate (SAS)-Margosa composite against toxigenic FFSC complex viz., F. fujikuroi, F. verticillioides, and F. proliferatum during storage of rice seeds with augemented antitoxin and seed invigorating potential. The novel SAS-Margosa composite was chemically and morphologically characterized using XRD, FTIR, TEM, and EDS analysis. The antifungal potential of margosa leaf extract adsorbed nanosilicate material (SAS) was examined using SEM, Flourescence microscopy and poison food technique. The toxin quantification of FFSC complex inoculated seeds treated with SAS-Margosa composite was carried out at monthly intervals upto a period of six months of storage along with control on two rice varieties viz., PR114 and Pusa Basmati 1121 respectively. The study revealed that SAS-Margosa composite coated seeds of PR114 and Pusa Basmati 1121 exhibited significanlty higher seed germination (87.63 & 83.57%) and minimum seed rot (5.70 & 5.24%) after 6 months of storage as compare to control. There was a significant decrease in levels of fusaric acid, fumonisins B1, B2 and gibberellic acid after six months as compare to control in both the varieties. The SAS-Margosa composite has been proved to be effective against toxigenic FFSC complex during rice seed storage without compromising the seed quality metrics.

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Cultural, Morphological and Molecular Characterization of Fusarium verticillioides causing Maize Ear Rot under Punjab Condition

Cited by 2 publications

References 10 publications

Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi

Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi

Seed coating with novel sodium alumino silicate margosa composite – A proficient technique to reduce mycotoxins of Fusarium fujikuroi species complex inciting discoloration of rice

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