Culture and characteristics of recombinant protein production of an Escherichia coli strain expressing carboxylesterase B1

Qiao, Chuanling; Shen, Buzhen; Xing, Jianmin; Huang, Jian; Zhang, J. L.; Zhao, Di; Yang, Bo-Hou

doi:10.1016/j.ibiod.2006.07.003

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…The probable reason for the low lipase activity with respect to high temperature is due to the aggregation of inclusion bodies. High temperature favours the aggregation of inclusion bodies whereas lower temperature enhances the secretion of recombinant protein in soluble form [24]. At lower temperature, reduced growth of E. coli [2] might be the reason for decreased lipase production.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the estimation capabilities of response surface methodology and artificial neural network for the optimization of recombinant lipase production by E. coli BL21

Nelofer

Ramanan

Rahman

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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Response surface methodology (RSM) and artificial neural network (ANN) were used to optimize the effect of four independent variables, viz. glucose, sodium chloride (NaCl), temperature and induction time, on lipase production by a recombinant Escherichia coli BL21. The optimization and prediction capabilities of RSM and ANN were then compared. RSM predicted the dependent variable with a good coefficient of correlation determination (R² and adjusted R² values for the model. Although the R (2) value showed a good fit, absolute average deviation (AAD) and root mean square error (RMSE) values did not support the accuracy of the model and this was due to the inferiority in predicting the values towards the edges of the design points. On the other hand, ANN-predicted values were closer to the observed values with better R², adjusted R², AAD and RMSE values and this was due to the capability of predicting the values throughout the selected range of the design points. Similar to RSM, ANN could also be used to rank the effect of variables. However, ANN could not predict the interactive effect between the variables as performed by RSM. The optimum levels for glucose, NaCl, temperature and induction time predicted by RSM are 32 g/L, 5 g/L, 32°C and 2.12 h, and those by ANN are 25 g/L, 3 g/L, 30°C and 2 h, respectively. The ANN-predicted optimal levels gave higher lipase activity (55.8 IU/mL) as compared to RSM-predicted levels (50.2 IU/mL) and the predicted lipase activity was also closer to the observed data at these levels, suggesting that ANN is a better optimization method than RSM for lipase production by the recombinant strain.

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Section: Discussionmentioning

confidence: 99%

Comparison of the estimation capabilities of response surface methodology and artificial neural network for the optimization of recombinant lipase production by E. coli BL21

Nelofer

Ramanan

Rahman

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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“…A low molecular weight SDS calibration kit containing proteins (14.4 -97.0 kDa) was used to provide standard molecular weight markers. Proteins were visualized by silver staining (17).…”

Section: Protein Determination and Electrophoresismentioning

confidence: 99%

Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli

Vaz¹,

Franca²,

Andrade³

et al. 2011

Braz. J. Microbiol.

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With the advent of recombinant DNA technology, recombinant protein expression has become an important tool in the study of the structure, function and identification of new proteins, especially those with therapeutic functions. Escherichia coli has been the predominant prokaryote used in genetic engineering studies due to the abundance of information about its metabolism. Despite significant advances in molecular biology and immunology of infections, there are as yet no prophylactic drugs capable of preventing visceral leishmaniasis. It is therefore important to identify specific antigens in order to develop vaccines and diagnostic kits against this disease. The objective of this study was to evaluate the influence of culture medium on the production of eIF antigen from Leishmania chagasi in recombinant Escherichia coli. An induction procedure using IPTG was carried out in a series of trials, to observe the influence of culture medium (2xTY, TB) under expression of the recombinant eIF protein. Results showed that recombinant protein expression was associated to growth and that the highest eIF antigen expression was obtained in the 2xTY medium.

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“…It has been reported that CarEs are involved in the mediation of metabolic resistance to organophosphate insecticides in several insect species. 15,29,31,33 The enhanced activity of CarEs in insects is frequently correlated with resistance to organophosphate pesticides, including chlorpyrifos and malathion. 15,31,32,34 In our study, a significant increase in CarE activity of B. odoriphaga after exposure to LC 30 of malathion, suggest that enhanced activity of CarE might contribute to detoxification of malathion.…”

Section: ■ Discussionmentioning

confidence: 99%

Functional Characterization of an α-Esterase Gene Associated with Malathion Detoxification in Bradysia odoriphaga

Tang

Dai

et al. 2020

J. Agric. Food Chem.

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Carboxylesterases (CarEs) are a multigene superfamily of metabolic enzymes involved in metabolic detoxification of xenobiotics. In this study, an α-esterase gene (BoαE1) was identified from Bradysia odoriphaga. Phylogenetic analysis classified BoαE1 into the α-esterase clade. Developmental expression analysis indicated that BoαE1 was significantly expressed in the second to fourth larval stages. Tissue-specific expression analysis indicated that BoαE1 was highly expressed in the larval midgut. After exposure to LC30 of malathion, the CarE activity of B. odoriphaga was induced and the transcriptional level of BoαE1 was significantly up-regulated. Silencing of BoαE1 significantly increased the susceptibility of B. odoriphaga larvae to malathion. Inhibition assays in vitro indicated that malathion significantly inhibited BoαE1 activity. GC-MS assay showed that BoαE1 possesses hydrolase activity toward malathion and participates in the detoxification of malathion. These results strongly suggest that BoαE1 plays a crucial role in detoxification of malathion in B. odoriphaga.

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Culture and characteristics of recombinant protein production of an Escherichia coli strain expressing carboxylesterase B1

Cited by 7 publications

References 16 publications

Comparison of the estimation capabilities of response surface methodology and artificial neural network for the optimization of recombinant lipase production by E. coli BL21

Comparison of the estimation capabilities of response surface methodology and artificial neural network for the optimization of recombinant lipase production by E. coli BL21

Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli

Functional Characterization of an α-Esterase Gene Associated with Malathion Detoxification in Bradysia odoriphaga

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