Culture and characterization of mesenchymal stem cells in a totally defined medium

Diet composition transiently modulates proliferative and potency features of human cord blood-derived mesenchymal stem cells

“…To investigate how behavior and properties of CBMSC are influenced by medium peculiarities, we chose two extremely different media as aMEM (Ragni et al, 2013b), one of the most widely used synthetic cell culture medium, and SPE-IV (Gadelorge et al, 2014), a recently released nutrient-rich medium containing supplemental cytokines (rh-IGF-1 and rh-b-FGF) and lipids that is reported by the supplier to confer higher proliferation rates maintaining stemness and differentiation properties in different MSC types. To avoid bias introduced by clones independently isolated with the two media and potentially displaying differential features (Halleux et al, 2001), MSC were first isolated in SPE-IV due to a higher isolation rate observed for CBMSC (Barilani et al, submitted).…”