Culture-Based Screening of Aerobic Microbiome in Diabetic Foot Subjects and Developing Non-healing Ulcers

Noor, Saba; Ahmad, Jamal; Parwez, Iqbal; Ozair, Maaz

doi:10.3389/fmicb.2016.01792

Cited by 19 publications

(19 citation statements)

References 27 publications

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“…Eight different species of bacteria were identified in total, varying from two to six different strains per subject (Fig 3b). This finding is consistent with data observed by other groups looking at this type of infection [16,17,48]. The culture conditions allowed us to isolate a variety of microorganisms.…”

Section: Diverse Microbiome Of a Grade 1b Ulcersupporting

confidence: 91%

Susceptibility of monomicrobial or polymicrobial biofilms derived from infected diabetic foot ulcers to topical or systemic antibiotics in vitro

et al. 2020

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Diabetic foot ulcers can become chronic and non-healing despite systemic antibiotic treatment. The penetration of systematically-administered antibiotics to the site of infection is uncertain, as is the effectiveness of such levels against polymicrobial biofilms. We have developed an in vitro model to study the effectiveness of different treatments for infected diabetic foot ulcers in a wound-like environment and compared the activity of systemic levels of antibiotics with that for topically applied antibiotics released from calcium sulfate beads. This is the first study that has harvested bacteria from diabetic foot infections and recreated similar polymicrobial biofilms to those present in vivo for individual subjects. After treatment with levels of gentamicin attained in serum after systemic administration (higher than corresponding tissues concentrations) we measured a 0-2 log reduction in bacterial viability of P. aeruginosa, S. aureus or a polymicrobial biofilm. Conversely, addition of gentamicin loaded calcium sulfate beads resulted in 5-9 log reductions in P. aeruginosa, S aureus and polymicrobial biofilms derived from three subjects. We conclude that systemically administered antibiotics are likely to be inadequate for successfully treating these infections, especially given the vastly increased concentrations required to inhibit cells in a biofilm, and that topical antibiotics provide a more effective alternative. OPEN ACCESSCitation: Price BL, Morley R, Bowling FL, Lovering AM, Dobson CB (2020) Susceptibility of monomicrobial or polymicrobial biofilms derived from infected diabetic foot ulcers to topical or systemic antibiotics in vitro. PLoS ONE 15(2): e0228704. https://doi.org/10.

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Section: Diverse Microbiome Of a Grade 1b Ulcersupporting

confidence: 91%

Susceptibility of monomicrobial or polymicrobial biofilms derived from infected diabetic foot ulcers to topical or systemic antibiotics in vitro

et al. 2020

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“…There have been several studies regarding the microbiomes of skin surface lesions, particularly in skin diseases such as atopic dermatitis, acne and chronic wounds, the severity of which were found to be related to the degree of bacterial colonization . Focusing on the genus level, the relative abundance of Staphylococcus sequences is greater during disease flares compared with non‐lesional skin and is correlated with increased severity of atopic dermatitis, acne and rosacea; it has been suggested that skin inflammation affects the characteristics of bacterial communities .…”

Section: Discussionmentioning

confidence: 99%

The microbiome of the “sterile” pustules in palmoplantar pustulosis

Masuda-Kuroki

Murakami

Tokunaga

et al. 2018

Experimental Dermatology

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The skin microbiome influences skin pathophysiology. Palmoplantar pustulosis (PPP) is a chronic skin disease characterized by infectious‐like pustules on the palms and soles. These pustules are thought to be sterile because bacterial cultures obtained from the pustules are negative. However, culture methods are limited in their ability to identify all bacteria on the skin. We hypothesized that the “sterile” pustules of PPP do not lack bacteria, but rather contain a microbiome. To test this hypothesis, we identified bacteria in “sterile” pustules using non‐culture methods. We conducted Sanger and 16S rRNA sequencing using primers specific to the V1‐V2 region in PPP‐pustulovesicles (PVs) (n = 43) and pompholyx vesicle fluids (n = 15). Sanger sequencing identified some Staphylococcus, Propionibacterium, Streptococcus and Pyrinomonas species in PPP‐PVs but failed to identify any bacteria in most of the pompholyx vesicles. 16S rRNA sequencing of PPP‐PVs indicated the presence of a microbiome that included various phyla, including Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes. At the genus level, smokers had higher levels of Staphylococcus in PPP‐PVs compared with non‐smokers. These results indicate that a microbiome exists in “sterile” pustules of PPP and that PPP smokers had higher levels of Staphylococcus in pustules. It is therefore necessary to reconsider the pathogenesis of PPP from the perspective of the microbiome.

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“…This trend was similar to our study, in which patients with Pseudomonas in the “≥HCs” group had a higher level of IL-8. Interestingly, Pseudomonas aeruginosa were the predominant isolates of non-healing ulcers in diabetic foot patients ( 45 ), and diabetic foot infection has demonstrated higher concentrations of IL-6 and IL-1β than controls ( 46 ). Thus, Pseudomonas might be correlated to the inflammatory process in diabetic patients and might regulate the levels of cytokines.…”

Section: Discussionmentioning

confidence: 99%

Dysbiosis of the Urinary Microbiota Associated With Urine Levels of Proinflammatory Chemokine Interleukin-8 in Female Type 2 Diabetic Patients

et al. 2017

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Evidence has shown that dysbiosis of the urinary microbiota existed in female type 2 diabetes mellitus (T2DM) patients. Perturbations of intestinal microbiota are linked to proinflammatory chemokine interleukin-8 (IL-8); however, the correlations between urinary microbiota and IL-8 are not well studied. Here, we investigated the associations between the altered urinary microbiota and urinary IL-8 in female T2DM patients. A modified four-tube midstream urine technique was used to collect urine specimens from 70 female T2DM patients and 70 matched healthy controls (HCs). Bacterial genomic DNA from urine specimens was isolated using magnetic beads and the urinary microbiota was assessed using Illumina MiSeq platform targeting on the 16S rRNA gene V3–V4 region. Urinary IL-8 was determined by enzyme linked immunosorbent assay. Subsequently, the T2DM patients were separated into urine IL-8 detectable (WIL8) and undetectable (NIL8) groups, and the composition of urinary microbiota between the two groups was compared. Meanwhile, the levels of IL-8 between the “≥HCs” group (those specific bacterial genera were more than or equal to the HCs) and the “

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Culture-Based Screening of Aerobic Microbiome in Diabetic Foot Subjects and Developing Non-healing Ulcers

Cited by 19 publications

References 27 publications

Susceptibility of monomicrobial or polymicrobial biofilms derived from infected diabetic foot ulcers to topical or systemic antibiotics in vitro

Susceptibility of monomicrobial or polymicrobial biofilms derived from infected diabetic foot ulcers to topical or systemic antibiotics in vitro

The microbiome of the “sterile” pustules in palmoplantar pustulosis

Dysbiosis of the Urinary Microbiota Associated With Urine Levels of Proinflammatory Chemokine Interleukin-8 in Female Type 2 Diabetic Patients

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