Culture Condition of Bone Marrow Stromal Cells Affects Quantity and Quality of the Extracellular Vesicles

Scheiber, Amanda L.; Clark, Cierra A.; Kaito, Takashi; Iwamoto, Masahiro; Horwitz, Edwin M.; Kawasawa, Yuka Imamura; Otsuru, Satoru

doi:10.3390/ijms23031017

Cited by 8 publications

(7 citation statements)

References 70 publications

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“…Similarly, donor variability has been identified in MSC-EVs from different donors. Proteomic and transcriptomic analyses of MSC-EVs from different donors demonstrated differences in their contents ( McBride et al, 2021 ; Ragni et al, 2019 ; Scheiber et al, 2022 ). These differences in cargo lead to the functional alteration of MSC-EVs, raising the challenge of reproducibility of EV therapies using EVs from different donors ( Chance et al, 2019 ; Kordelas et al, 2014 ; Kordelas et al, 2019 ).…”

Section: Key Elements That Can Affect Ev Qualitymentioning

confidence: 99%

“…Since there is no raw material derived from animals or humans, such media will be more consistent between lots, which potentially makes EV quality more reproducible. Xeno-free media not only helps the rapid expansion of MSCs but also yields significantly greater amounts of EVs compared to conventional FBS-containing media ( Scheiber et al, 2022 ). However, miRNA profiles in EVs from such media are altered ( Scheiber et al, 2022 ).…”

Section: Key Elements That Can Affect Ev Qualitymentioning

confidence: 99%

“…Xeno-free media not only helps the rapid expansion of MSCs but also yields significantly greater amounts of EVs compared to conventional FBS-containing media ( Scheiber et al, 2022 ). However, miRNA profiles in EVs from such media are altered ( Scheiber et al, 2022 ).…”

Section: Key Elements That Can Affect Ev Qualitymentioning

confidence: 99%

“…EVs have been demonstrated to contain a complex array of bioactive molecules that includes proteins ( Tkach and Théry, 2016 ), lipids ( Skotland et al, 2017 ), and nucleic acids such as messenger RNA (mRNA) ( Valadi et al, 2007 ), micro RNA (miRNA) ( Nazari-Shafti et al, 2020 ; Scheiber et al, 2022 ), long noncoding RNA (lncRNA) ( Wen et al, 2022 ), transfer RNA (tRNA) ( Baglio et al, 2015 ), genomic DNA( Kahlert et al, 2014 ; Thakur et al, 2014 ), and mitochondrial DNA (mtDNA) ( Guescini et al, 2010 ). EVs mediate intercellular communication by transferring such cargo to each other ( Tkach and Théry, 2016 ; Valadi et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

“…In addition to the quality of EVs, the quantity must be considered for clinical usage involving multiple injections throughout the treatment ( Adlerz, 2020 ; Colao et al, 2018 ; Phinney and Pittenger, 2017 ). However, scalable manufacturing of EVs also affects the quality of EVs ( Haraszti et al, 2018 ; Patel et al, 2017 ; Scheiber et al, 2022 ). Therefore, in this review, to overcome these challenges and achieve effective and reliable EV therapy, we highlight the key elements that can affect EV quality followed by a summary of the pre-clinical and clinical EV studies surrounding skeletal diseases.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

MSC-EV therapy for bone/cartilage diseases

Kodama¹,

Wilkinson²,

Otsuru³

2022

Bone Reports

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Section: Key Elements That Can Affect Ev Qualitymentioning

confidence: 99%

Section: Key Elements That Can Affect Ev Qualitymentioning

confidence: 99%

Section: Key Elements That Can Affect Ev Qualitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

MSC-EV therapy for bone/cartilage diseases

Kodama¹,

Wilkinson²,

Otsuru³

2022

Bone Reports

Self Cite

View full text Add to dashboard Cite

Cell‐Based Biomaterials for Coronavirus Disease 2019 Prevention and Therapy

Wang

Xie

et al. 2023

Adv Healthcare Materials

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Coronavirus disease 2019 (COVID-19) continues to threaten human health, economic development, and national security. Although many vaccines and drugs have been explored to fight against the major pandemic, their efficacy and safety still need to be improved. Cell-based biomaterials, especially living cells, extracellular vesicles, and cell membranes, offer great potential in preventing and treating COVID-19 owing to their versatility and unique biological functions. In this review, the characteristics and functions of cell-based biomaterials and their biological applications in COVID-19 prevention and therapy are described. First the pathological features of COVID-19 are summarized, providing enlightenment on how to fight against COVID-19. Next, the classification, organization structure, characteristics, and functions of cell-based biomaterials are focused on. Finally, the progress of cell-based biomaterials in overcoming COVID-19 in different aspects, including the prevention of viral infection, inhibition of viral proliferation, anti-inflammation, tissue repair, and alleviation of lymphopenia are comprehensively described. At the end of this review, a look forward to the challenges of this aspect is presented.

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The proteomic landscape of extracellular vesicles derived from human intervertebral disc cells

Li,

Al‐Jallad,

Sun

et al. 2024

JOR Spine

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BackgroundExtracellular vesicles (EVs) function as biomarkers and are crucial in cell communication and regulation, with therapeutic potential for intervertebral disc (IVD)‐related low back pain (LBP). EV cargo is often affected by tissue health, which may affect the therapeutic potential. There is currently limited knowledge of how the cargo of IVD cell‐derived EVs varies with tissue health and how differences in proteomic profile affect the predicted biological functions.MethodsOur study purified EVs from human IVD cell conditioned media by size‐exclusion chromatography. Nanoparticle tracking analysis was conducted to measure EV size and concentration. Transmission electron microscopy and Western blot were performed to examine EV structure and markers. Tandem mass tag‐mass spectrometry was conducted to determine protein cargo.ResultsMost EVs were exosomes and intermediate microvesicles with an increasing amount linked to disease progression. Of the proteins detected, 88.6% were shared across the non‐degenerate, mildly‐degenerate, and degenerate samples. GO and KEGG analyses revealed that cargo from the mildly‐degenerate samples was the most distinct, with the proteins in high abundance strongly associated with extracellular matrix (ECM) organization and structure. Shared proteins, highly expressed in the non‐degenerate and degenerate samples, showed strong associations with cell adhesion, ECM–receptor interaction, and vesicle‐mediated transport, respectively.ConclusionsOur findings indicate that EVs from IVD cells from tissue with different degrees of degeneration share a majority of the cargo proteins. However, the level of expression differs with degeneration grade. Cargo from the mildly‐degenerate samples exhibits the most differences. A better understanding of changes in EV cargo in the degenerative process may provide novel information related to molecular mechanisms underlying IVD degeneration and suggest new potential treatment modalities for IVD‐related LBP.

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Culture Condition of Bone Marrow Stromal Cells Affects Quantity and Quality of the Extracellular Vesicles

Cited by 8 publications

References 70 publications

MSC-EV therapy for bone/cartilage diseases

MSC-EV therapy for bone/cartilage diseases

Cell‐Based Biomaterials for Coronavirus Disease 2019 Prevention and Therapy

The proteomic landscape of extracellular vesicles derived from human intervertebral disc cells

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