Culture Conditions for Improvement ofL-Threonine Production Using a Genetically Self-clonedL-Threonine Hyperproducing Strain ofEscherichia coliK-12

Metabolic pathways and fermentative production of L‐aspartate family amino acids

“…A high level of dissolved oxygen and lowering the required amino acids were found to be effective for increasing L-threonine production. By properly adjusting these conditions, a final L-threonine concentration of 65 g/L was obtained, resulting in a yield of 0.48 g/g glucose [55], which is 59.3% of the maximum theoretical yield of L-threonine. The theoretical maximum achievable yield of L-threonine was calculated to be 0.81 g/g glucose, which can be achieved if there is no biomass formation [48].…”

Disruption ofmetFIncreased L-Lysine Production byMethylophilus methylotrophusfrom Methanol

“…We have obtained useful mutations in genes encoding key enzymes of L-lysine biosynthesis in E. coli, 22,23) and practical fermentation process techniques for amino acid fermentation. [24][25][26] We believe that L-lysine production in M. methylotrophus 102/pSEA10 can be further enhanced by the use of our results and techniques.…”

Kinetic analysis and modeling of L-valine production in fermentation batch from E. coli using glucose, lactose and whey as carbon sources

“…Volumes were adjusted so the inoculate concentration was 0.2 g dry cells/L at the beginning of the fermentation. Fermentation in the bioreactor was performed at 37 °C with pH control at 6.9, adjusted with NaOH (2 M); dissolved oxygen level was kept approximately at 20 % saturation by agitation speed adjustment between 300 rpm and 600 rpm [ 27 , 28 ] for 24 h. Sampling was carried out every 3 h to measure optical density (OD) and subsequent sugar (glucose and lactose) and L-valine analysis after centrifugation at 10,000 rpm for 9 min at 4 °C.…”