Culture conditions influencing phytase production of<i>Mitsuokella jalaludinii</i>, a new bacterial species from the rumen of cattle

Lan, Ganqiu; Abdullah, Norhani; Jalaludin, S.; Ho, Yin Wan

doi:10.1046/j.1365-2672.2002.01727.x

Cited by 47 publications

(29 citation statements)

References 22 publications

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“…One of these species is Mitsuokella jalaludinii, significantly depleted after the HFS treatment. Mitsuokella jalaludinii, has been recently isolated from the rumen of cattle (Lan et al, 2002) and is a member of the Veillonellaceae family that has been previously demonstrated to be reduced in HFD-fed rats (Yin et al, 2013). In our experiment, Veillonellaceae did not statistically differ between groups but, as observed in the PCA, it was represented in a greater extent in HFS diet-fed rats.…”

Section: Discussionsupporting

confidence: 53%

Shifts in microbiota species and fermentation products in a dietary model enriched in fat and sucrose

Etxeberría

Arias

Boqué

et al. 2015

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The gastrointestinal tract harbours a "superorganism" called the gut microbiota, which is known to play a crucial role in the onset and development of diverse diseases. This internal ecosystem, far from being a static environment, could be willingly manipulated by diet and dietary components. Feeding animals with high-fat sucrose diets entails diet-induced obesity, a model which is usually used in research to mimic the obese phenotype of Western societies.The aim of the present study was to identify gut microbiota dysbiosis and associated metabolic changes produced in 5 male Wistar rats fed a high-fat sucrose (HFS) diet for six weeks and to compare it with the basal microbial composition. For this purpose, DNA extracted from faeces at baseline and after the treatment was analysed by amplification of the V4-V6 region of the 16S ribosomal DNA (rDNA) gene using 454 pyrosequencing. Short-chain fatty acids (SCFA), acetate, propionate and butyrate, were also evaluated by gas chromatography-mass spectrometry (GC-MS).At the end of the treatment, gut microbiota composition significantly differed at phylum level (Firmicutes, Bacteroidetes and Proteobacteria) and class level (Erisypelotrichi, Deltaproteobacteria, Bacteroidia and Bacilli). Interestingly, Clostridia class showed a significant decrease after the HFS-diet treatment, which correlated with visceral adipose tissue, and is likely mediated by dietary carbohydrates. Of particular interest, Clostridium cluster XIVa species were significantly reduced and changes were identified in the relative abundance of other specific bacterial species (Mitsuokella jalaludinii, Eubacterium ventriosum, Clostridium sp. FCB90-3, Prevotella nanceiensis, Clostridium fusiformis, Clostridium sp. BNL1100 and Eubacterium cylindroides) that, in some cases, showed opposite trends to their relative families.These results highlight the relevance of characterizing gut microbial population differences at species level and contribute to understand the plausible link between the 1 diet and specific gut bacterial species that are able to influence the inflammatory status, intestinal barrier function and obesity development.

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Section: Discussionsupporting

confidence: 53%

Shifts in microbiota species and fermentation products in a dietary model enriched in fat and sucrose

Etxeberría

Arias

Boqué

et al. 2015

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“…Furthermore, the eVect of inoculum age (12,24,36,48,60, and 72 h) on the production of phytase was studied at optimal inoculum level of (2% v/v).…”

Section: Evect Of Inoculum Level and Age On The Production Of Phytasementioning

confidence: 99%

Production and characterization of thermostable alkaline phytase from Bacillus laevolacticus isolated from rhizosphere soil

Gulati

Chadha

Saini

2006

J Ind Microbiol Biotechnol

107

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Proteases are one of the largest groups of hydrolytic enzymes constituting about 60% of total worldwide sales of industrial enzymes due to their wide applications in detergent, leather, textile, food and pharmaceutical industry. Microbial proteases have been preferred over animal and plant proteases because of their fundamental features and ease in production. Bacillus infantis SKS1, an alkaline protease producing bacteria has been isolated from garden soil of north India and identified using morphological, biochemical and molecular methods. 16S rDNA sequence amplified using universal primers has 99% sequence identity with corresponding gene sequence of Bacillus infantis strain FM 34 and Bacillus sp. Beige. The bacterial culture and its 16S rDNA gene sequence have been deposited to Microbial Culture Collection (Pune, India) with accession number MCC 3035 and GenBank with accession number KR092197 respectively. The partially purified extract of Bacillus infantis SKS1 was thermostable and active in presence of Mg 2+ , acetyl acetone and laundry detergents implicating its application in industry. Production of these enzymes using this strain was maximized by optimization of various parameters including temperature, pH, media components and other growth conditions. Our results show that fructose and dextrose serve as the best carbon sources for production of these enzymes, highlighting the use of this strain for enzyme production utilizing relatively inexpensive substrates like beet molasses and corn steep liquor. Additionally, this strain showed maximum production of enzymes at 40˚C similar to bacterial species used for commercial production of alkaline proteases. Characterization of alkaline proteases from this strain of Bacillus infantis and optimization of parameters for its production would help in understanding its industrial application and large-scale production.

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“…The buffering capacity of phosphate may have a positive effect on phytase synthesis (Lan et al, 2002).…”

Section: Resultsmentioning

confidence: 99%

Enhancement of Phytase Production from a New Probiotic Strain Bacillus subtilis P6

Trivedi¹,

Sharma²,

Jain³

2017

Int.J.Curr.Microbiol.App.Sci

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Culture conditions influencing phytase production ofMitsuokella jalaludinii, a new bacterial species from the rumen of cattle

Cited by 47 publications

References 22 publications

Shifts in microbiota species and fermentation products in a dietary model enriched in fat and sucrose

Shifts in microbiota species and fermentation products in a dietary model enriched in fat and sucrose

Production and characterization of thermostable alkaline phytase from Bacillus laevolacticus isolated from rhizosphere soil

Enhancement of Phytase Production from a New Probiotic Strain Bacillus subtilis P6

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