Culture in embryonic kidney serum and xeno-free media as renal cell carcinoma and renal cell carcinoma cancer stem cells research model

Abstract

The use of fetal bovine serum hinders obtaining reproducible experimental results and should also be removed in hormone and growth factor studies. In particular hormones found in FBS act globally on cancer cell physiology and influence transcriptome and metabolome. The aim of our study was to develop a renal carcinoma serum free culture model optimized for (embryonal) renal cells in order to select the best study model for downstream auto-, para- or endocrine research. Secondary aim was to verify renal carcinoma stem cell culture for this application. In the study, we have cultured renal cell carcinoma primary tumour cell line (786-0) as well as human kidney cancer stem cells in standard 2D monolayer cultures in Roswell Park Memorial Institute Medium or Dulbecco’s Modified Eagle’s Medium and Complete Human Kidney Cancer Stem Cell Medium, respectively. Serum-free, animal-component free Human Embryonic Kidney 293 media were tested. Our results revealed that xeno-free embryonal renal cells optimized culture media provide a useful tool in RCC cancer biology research and at the same time enable effective growth of RCC. We propose bio-mimic RCC cell culture model with specific serum-free and xeno-free medium that promote RCC cell viability.

Development of extracellular matrix supported 3D culture of renal cancer cells and renal cancer stem cells

“…The growth of renal cancer and renal cancer stem cells in in vitro conditions requires optimal stability between signals mediating proliferation, cell survival and self-renewal. For in vitro application of RCC, the use of completely defined conditions and elimination of animal-derived materials from the culture is recommended (Krawczyk et al 2018 ). Therefore, we aimed to develop the most efficient animal serum free culture system for RCC experiments through comparing available stable RCC cell lines and verifying different culture media.…”

Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells

“…Emerging evidence has revealed that the addition of specific serum under specific conditions during the extraction of MSCs or the enrichment of tumor stem cells can have a positive effect [ 18 – 20 ]. It has also been shown that it is entirely possible to cultivate CSCs from serum-containing media [ 21 , 22 ]. In this study, we cultured HCT116 in ultra-low adhesion plates with serum-free medium containing EGF, FGF-2, and B-27.…”