Culture-Independent Detection of Poliovirus in Stool Samples by Direct RNA Extraction

Abstract

The GPLN is a global surveillance system composed of 146 laboratories in 92 countries, in each of the six World Health Organization regions. Laboratory surveillance for PV relies on virus isolation by cell culture to identify PV in stool samples from AFP cases.

Comparison of Eleven RNA Extraction Methods for Poliovirus Direct Molecular Detection in Stool Samples

“…This suggests that some of the stool samples had lost their integrity. Harrington et al found PV direct detection following RNA extraction to be significantly more sensitive than virus isolation by cell culture for PV detection in retrospective stool samples ( 21 ). In this study, the PV-positive stool suspensions were only tested with direct RNA extraction methods for PV molecular detection.…”

Safety of the novel oral poliovirus vaccine type 2 (nOPV2) in infants and young children aged 1 to <5 years and lot-to-lot consistency of the immune response to nOPV2 in infants in The Gambia: a phase 3, double-blind, randomised controlled trial

“…Serotype-specific polioviruses were detected in the stool using a validated real-time RT-PCR assay. 18 Laboratory analysis was conducted at the US Centers for Disease Control and Prevention ( appendix p 8 ).…”

The Last Mile in Polio Eradication: Program Challenges and Perseverance

“…There are two alternative direct detection technologies currently being assessed, namely (i) direct detection with intratypic differentiation (DD-ITD) and (ii) direct detection nanopore sequencing (DDNS) [ 45 , 46 ]. These methods are faster and safer alternatives compared to standard cell culture regarding containment-related risks associated with growing live viruses.…”