Culture Media Composition Influences the Antibacterial Effect of Silver, Cupric, and Zinc Ions against Pseudomonas aeruginosa

Rewak‐Soroczyńska, Justyna; Dorotkiewicz-Jach, Agata; Drulis-Kawa, Zuzanna; Wiglusz, Rafał J.

doi:10.3390/biom12070963

Cited by 19 publications

(4 citation statements)

References 39 publications

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“…The MIC of allicin, a compound extracted from garlic, was also shown to be media-dependent, resulting from its stability and availability in different media [ 41 ]. Other studies showed that the pH of the growth medium can have a significant effect on antimicrobial activity [ 42 ]; however, the pH of MHB and TSB used in this study is typically around pH 7.3 for both [ 43 ], suggesting that pH was not a factor in this case. Furthermore, Buyck et al (2012) [ 44 ] showed that the MIC of the antibiotic azithromycin against Pseudomonas aeruginosa was much lower in eukaryotic cell culture medium compared to MHB, resulting from decreased expression of the opr M gene as well as increased permeability of the outer membrane.…”

Section: Discussionmentioning

confidence: 82%

Antibacterial and Antibiofilm Activity of Endophytic Alternaria sp. Isolated from Eremophila longifolia

Caruso,

Palombo,

Moulton

et al. 2023

Antibiotics

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The threat to public health resulting from the emergence of antimicrobial resistance (AMR) is ever rising. One of the major bacterial pathogens at the forefront of this problem is methicillin-resistant Staphylococcus aureus, or MRSA, for which there is a great need to find alternative treatments. One of the most promising alternatives is endophytic fungi, which were shown to produce a vast array of bioactive compounds, including many novel antibacterial compounds. In this study, two endophytic Alternaria sp., EL 24 and EL 35, were identified from the leaves of Eremophila longifolia. Ethyl acetate (EtOAc) extracts of their culture filtrates were found to inhibit both methicillin-sensitive S. aureus ATCC 25923 and MRSA strains M173525 and M180920. The activity of each extract was shown to be greatly affected by the growth medium, with considerable reductions in minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) observed when tested in tryptic soy broth with glucose (TSBG) compared with Mueller–Hinton broth (MHB). Both extracts displayed significant (p ≤ 0.05) antibiofilm activity against all three S. aureus strains, the greatest of which was that of EL 35, which reduced biofilm formation by M180920 by 72%, while that of EL 24 resulted in a 57% reduction against ATCC 25923. Both extracts also disrupted established biofilms, of which the most effective was EL 35, which reduced the M180920 biofilm by 64%, while EL 24 also performed best against M180920, reducing biofilm by 54%. Gas chromatography–mass spectrometry (GC-MS) analysis of the EL 24 EtOAc extract revealed five known compounds. This study highlights the promise of endophytic fungi from Australian plants as a potential source of substances effective against important bacterial pathogens. Further understanding of the responsible compounds and their mechanisms could lead to the development of treatments effective against MRSA, as well as novel biofilm-resistant biomedical materials, contributing towards reducing the burden of AMR.

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Section: Discussionmentioning

confidence: 82%

Antibacterial and Antibiofilm Activity of Endophytic Alternaria sp. Isolated from Eremophila longifolia

Caruso,

Palombo,

Moulton

et al. 2023

Antibiotics

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“…On the other hand, Muller Hinton broth is much more complex, having all nutrients necessary for bacterial growth. An experiment on how different metal ions interact with different culture media [58] revealed that, silver ions are quite active in both simple and more complex media, producing precipitates (when in M9 media, Ag + ions precipitate to AgCl). It could be deduced that the more nutritious the medium, the more compounds there are for silver to interact with, thus reducing its antibacterial properties.…”

Section: Broth Dilution Methodsmentioning

confidence: 99%

Untitled

2022

AnnalsARSciPhysChem

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Synthetic hydroxyapatite (Ca10(PO4)6(OH)2, HAP) is widely used in regards to orthopedic applications due to its similarity to the mineral component in bone. However, while HAP aids with osseointegration it does nothing when it comes to orthopedic infections. Moreover, the prevalence of antibiotic resistance makes treatment even more difficult. In view of this, adding silver to hydroxyapatite has been a focus of many studies due to the combined bioactivity of HAP and excellent antibacterial properties of Ag. The present work brings a brief introduction to more recent studies regarding the HAP-silver combination and its effect on different pathogenic strains. The effect of silver on benign cells is also discussed based on in vitro cultures and in vivo studies.

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“…As already mentioned, zinc ions exhibit antimicrobial activity that affects both Gram-positive and Gram-negative species, including Staphylococcus epidermidis , S. aureus , Streptococcus mutans , Pseudomonas aeruginosa and Escherichia coli . Moreover, Zn 2+ ions were reported to reduce Candida albicans biofilm [ 5 , 22 , 23 ]. Zn 2+ -doped HAp also promotes osteoblasts proliferation and viability [ 5 , 17 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

The Study of Nanosized Silicate-Substituted Hydroxyapatites Co-Doped with Sr2+ and Zn2+ Ions Related to Their Influence on Biological Activities

Rewak‐Soroczyńska

Nowak

Targońska

et al. 2022

CIMB

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Nanosized silicate-substituted hydroxyapatites, characterized by the general formula Ca9.8−x−nSrnZnx(PO4)6−y(SiO4)y(OH)2 (where: n = 0.2 [mol%]; x = 0.5–3.5 [mol%]; y = 4–5 [mol%]), co-doped with Zn2+ and Sr2+ ions, were synthesized with the help of a microwave-assisted hydrothermal technique. The structural properties were determined using XRD (X-ray powder diffraction) and Fourier-transformed infrared spectroscopy (FT-IR). The morphology, size and shape of biomaterials were detected using scanning electron microscopy techniques (SEM). The reference strains of Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa were used to assess bacterial survivability and the impact on biofilm formation in the presence of nanosilicate-substituted strontium-hydroxyapatites. Safety evaluation was also performed using the standard cytotoxicity test (MTT) and hemolysis assay. Moreover, the mutagenic potential of the materials was assessed (Ames test). The obtained results suggest the dose-dependent antibacterial activity of nanomaterials, especially observed for samples doped with 3.5 mol% Zn2+ ions. Moreover, the modification with five SiO4 groups enhanced the antibacterial effect; however, a rise in the toxicity was observed as well. No harmful activity was detected in the hemolysis assay as well as in the mutagenic assay (Ames test).

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Culture Media Composition Influences the Antibacterial Effect of Silver, Cupric, and Zinc Ions against Pseudomonas aeruginosa

Cited by 19 publications

References 39 publications

Antibacterial and Antibiofilm Activity of Endophytic Alternaria sp. Isolated from Eremophila longifolia

Antibacterial and Antibiofilm Activity of Endophytic Alternaria sp. Isolated from Eremophila longifolia

Untitled

The Study of Nanosized Silicate-Substituted Hydroxyapatites Co-Doped with Sr2+ and Zn2+ Ions Related to Their Influence on Biological Activities

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