Culture medium pH influence on Gluconacetobacter physiology: Cellulose production rate and yield enhancement in presence of multiple carbon sources

Yassine, Fatima; Bassil, Nathalie; Flouty, Roula; Chokr, Ali; Samrani, Antoine El; Boiteux, Gisèle; Tahchi, Mario El

doi:10.1016/j.carbpol.2016.02.003

Cited by 19 publications

(11 citation statements)

References 49 publications

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“…Yassine et al. found that the addition of acetic acid to the culture medium before inoculation could limit the decrease in the pH level of culture. It indicated that the presence of acetic acid in the basal media could limit organic acid production and accumulation of strain JR‐02 and keep the culture in the optimal condition for BC production.…”

Section: Resultsmentioning

confidence: 99%

Production of high crystallinity type‐I cellulose from Komagataeibacter hansenii JR‐02 isolated from Kombucha tea

Chen

Zhang

et al. 2018

Biotech and App Biochem

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In this study, a bacterial cellulose (BC) producing strain was isolated from Kombucha tea and identified as Komagataeibacter hansenii JR-02 by morphological, physiological, and biochemical characterization and 16S rRNA sequence. Then, the media components and culture conditions for BC production were optimized. Result showed that the highest BC yield was 3.14 ± 0.22 and 8.36 ± 0.19 g/L after fermentation for 7 days under shaking and static cultivation, respectively. Moreover, it was interesting that JR-02 could produce BC in nitrogen-free medium with the highest yield of 0.76 ± 0.06 g/L/7days, and the possible nitrogen fixation gene nifH was cloned from its genomic DNA.The BC produced by JR-02 was type-I cellulose with high crystallinity and thermodynamic stability, which was revealed from Fourier transform infrared spectroscopy, X-ray diffraction, and thermogravimetric analysis methods. The crystallinity of static and shaking cultured BC were 91.76% and 90.69%, respectively. The maximum rate of weight loss of static and shaking BC occurred at temperature of approximately 373.1 • C and 369.1 • C, respectively. Overall, these results indicated that K. hansenii JR-02 had great potential to produce high crystallinity type-I BC in manufacture.Abbreviations: β, β is the line broadening at half the maximum intensity (FWHM) in radians; λ, λ is the X-ray wavelength (1.54Å); θ , θ is the Bragg's angle; +, the experiment result is positive; −, the experiment result is negative; (+), the experiment result is weak positive; BC, bacterial cellulose; Cel+, BC producing strains; Cel−, BC producing strains lost the function of bacterial cellulose producing; CrI, crystallinity index; CrS, crystallite size; CSL, corn steep liquor; d-spacing, interplanar crystal distance; DTG, differential thermal analysis; FE-SEM, Field emission scanning electron microscopy; FTIR, fourier transform infrared; Iam, the minimum intensity between (101) and (002) peaks; I002, I002 is the intensity at (002) peak which is showed in the XRD profiles of BC; k, k is the shape factor (0.9); nr, The experiment result is not reported; SK, BC obtained from shaking culture; ST, BC obtained from static culture; TCA, tricarboxylic acid cycle; TGA, thermogravimetric analysis; UDPG, uridine 5 -diphosphate glucose; XRD, X-ray diffractometry; YE, yeast extract. .

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Section: Resultsmentioning

confidence: 99%

Production of high crystallinity type‐I cellulose from Komagataeibacter hansenii JR‐02 isolated from Kombucha tea

Chen

Zhang

et al. 2018

Biotech and App Biochem

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“…In addition, the use of BC for industrial and medical purposes is limited due to the low conversion rate of nutrients in cellulose and the long culture time (Yassine et al . ).…”

Section: Introductionmentioning

confidence: 97%

Influence of chemical and physical conditions in selection ofGluconacetobacter hanseniiATCC 23769 strains with high capacity to produce bacterial cellulose for application as sustained antimicrobial drug-release supports

et al. 2018

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“…For this purpose, biochemical metabolism tests such as API 20 CH or the API 50 CH are used in clinical settings (Cyprowski et al 2018). Another study used McIlvaine buffer to show the pH influence of the culture medium on the Gluconacetobacter physiology (Yassine et al 2016).…”

Section: Identification Of Culturable Bioaerosolsmentioning

confidence: 99%

Assays and enumeration of bioaerosols-traditional approaches to modern practices

King

Lacey

Pak

et al. 2020

Aerosol Science and Technology

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In recent years, much attention has been drawn to the exposure to bioaerosols in both occupational and indoor environments due to adverse effects on human health. Exposure to these agents may cause infectious diseases, allergic diseases, acute toxic effects, respiratory diseases, neurological effects, and cancer. Bioaerosols play a significant role in air quality studies, as well as in industrial and agricultural regulations. There is a diversity of sources for bioaerosol exposures, which include occupational activities such as waste disposal, sorting and composting, agricultural and food processing, livestock production and handling, healthcare, and fungal growth following flooding. Bioaerosol monitoring includes the measurement of viable (culturable and nonculturable) and nonviable microorganisms in both indoor and outdoor environments. The development of standardized methods for the detection and quantitation of bioaerosols has become an important issue. Technologies, including microbial plating, physical-chemical assays, and molecular techniques, have been adapted for the assessment of collected bioaerosol particles. In this review, we will discuss traditional and modern assays that have been applied in bioaerosol enumeration based on culturability, optical properties, immunoreactions, and nucleic acid amplification and sequencing of biological particles. The analysis of additional characteristics of bioaerosols including microbiome and antimicrobial resistance is also discussed. Finally, concluding thoughts are offered regarding the challenges and perspectives in the field.

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Culture medium pH influence on Gluconacetobacter physiology: Cellulose production rate and yield enhancement in presence of multiple carbon sources

Cited by 19 publications

References 49 publications

Production of high crystallinity type‐I cellulose from Komagataeibacter hansenii JR‐02 isolated from Kombucha tea

Production of high crystallinity type‐I cellulose from Komagataeibacter hansenii JR‐02 isolated from Kombucha tea

Influence of chemical and physical conditions in selection ofGluconacetobacter hanseniiATCC 23769 strains with high capacity to produce bacterial cellulose for application as sustained antimicrobial drug-release supports

Assays and enumeration of bioaerosols-traditional approaches to modern practices

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