Culture of a Gastric Non‐<i><scp>H</scp>elicobacter pylori</i> Helicobacter from the Stomach of a 14‐Year‐Old Girl

Wüppenhorst, Nicole; Loewenich, Friederike von; Hobmaier, Beate; Vetter-Knoll, Marianne; Mohadjer, Sona; Kist, Manfred

doi:10.1111/j.1523-5378.2012.00990.x

Cited by 12 publications

(16 citation statements)

References 22 publications

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“…infection in most patients [3,5,10-13]. However, cases of failed treatment have been reported [7,14]. The peculiar growth requirements of H. heilmannii s.l.…”

Section: Introductionmentioning

confidence: 99%

Contingency nature of Helicobacter bizzozeronii oxygen-insensitive NAD(P)H-nitroreductase (HBZC1_00960) and its role in metronidazole resistance

Kondadi

Pacini

Revez

et al. 2013

Vet Res

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Genomic analysis of a metronidazole resistant H. bizzozeronii strain revealed a frame length extension of the oxygen-insensitive NAD(P)H-nitroreductase HBZC1_00960 (RdxA), associated with the disruption of the C-terminal cysteine-containing conserved region (IACLXALGK). This was the result of the extension (from C8 to C9) of a simple sequence cytosine repeat (SSCR) located in the 3’ of the gene. A 3' SSCR is also present in the rdxA homolog of H. heilmannii sensu stricto, but not in H. pylori. We showed that in the majority of in vitro spontaneous H. bizzozeronii metronidazole resistant mutants, the extension of the 3′ SSCR of rdxA was the only mutation observed. In addition, we observed that H. bizzozeronii ΔrdxA mutant strain showed the same MIC value of metronidazole observed in the spontaneous mutants. These data indicate that loss of function mutations in rdxA and in particular the disruption of the conserved region IACLXALGK is associated with reduced susceptibility to metronidazole in H. bizzozeronii. Slipped-strand mispairing of the SSCR located in the 3′ of the H. bizzozeronii rdxA appears to be the main mechanism. We also observed that H. bizzozeronii acquires resistance to metronidazole at high mutation rate, and that serial passages in vitro without selection induced an increased level of susceptibility. In conclusion, contrary to what was previously described in H. pylori, the H. bizzozeronii rdxA appears to be a contingency gene which undergoes phase variation. The contingency nature of rdxA should be carefully considered when metronidazole is used in the treatment of H. heilmannii-associated gastritis.

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“…infection in most patients [3,5,10-13]. However, cases of failed treatment have been reported [7,14]. The peculiar growth requirements of H. heilmannii s.l.…”

Section: Introductionmentioning

confidence: 99%

Contingency nature of Helicobacter bizzozeronii oxygen-insensitive NAD(P)H-nitroreductase (HBZC1_00960) and its role in metronidazole resistance

Kondadi

Pacini

Revez

et al. 2013

Vet Res

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“…So far, very few laboratories have succeeded in the isolation of H. heilmannii s.l. from the gastric mucosa of cats, dogs, or pigs [15,44,45,67] and only H. bizzozeronii and H. felis have been isolated from the human gastric mucosa [35,[68][69][70] . Currently, the most accurate method available for conclusive species identification is the use of PCR, followed by sequencing of specific target genes.…”

Section: Diagnosismentioning

confidence: 99%

Helicobacter heilmannii sensu lato: An overview of the infection in humans

Bento-Miranda

Figueiredo

2014

WJG

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Helicobacter heilmannii sensu lato (H. heilmannii s.l.) is a group of gastric non-Helicobacter pylori Helicobacter species that are morphologically indistinguishable from each other. H. heilmannii s.l. infect the stomach of several animals and may have zoonotic potential. Although the prevalence of these infections in humans is low, they are associated with gastric pathology, including mucosa-associated lymphoid tissue lymphoma, making them a significant health issue. Here, the taxonomy, epidemiology, microbiology, diagnosis, and treatment of these infections will be reviewed. The gastric pathology associated with H. heilmannii s.l. infections in humans will also be addressed. Finally, the features of the complete bacterial genomes available and studies on species-specific pathogenesis will be reviewed. The understanding of the mechanisms that underlie gastric disease development mediated by the different bacterial species that constitute H. heilmannii s.l. is essential for developing strategies for prevention and treatment of these infections. Core tip: Helicobacter heilmannii sensu lato is a group of non-Helicobacter pylori Helicobacter species that infect the stomach of animals and humans. In the human stomach, these infections are associated with several pathologies, but it is currently unknown whether certain species are more often associated with a certain disease outcome than others. The access to bacterial genomes together with the availability of increasing numbers of in vitro isolates will allow significant advances in the understanding of species-specific bacteria-host interactions in disease pathogenesis and will be essential for future development of strategies to prevent and treat these infections.

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“…Besides H. pylori, non-Helicobacter pylori Helicobacters (NHPHs) are detected in 0.2-6% of human patients undergoing a gastroscopy, in general by histopathologic analysis or molecular methods [3][4][5]. Gastric NHPH infection in humans has been suggested to occur through zoonotic transmission [6,7], and infection has been associated with the development of gastritis, gastric ulceration, and gastric MALT lymphoma [8].…”

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confidence: 99%

“…isolates have been cultured from human gastric biopsy specimen [5,14,15]. group from humans is extremely difficult.…”

mentioning

confidence: 99%

Prevalence of Coinfection with Gastric Non‐Helicobacter pylori Helicobacter (NHPH) Species in Helicobacter pylori‐infected Patients Suffering from Gastric Disease in Beijing, China

Liu

Haesebrouck

et al. 2014

Helicobacter

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Culture of a Gastric Non‐Helicobacter pylori Helicobacter from the Stomach of a 14‐Year‐Old Girl

Cited by 12 publications

References 22 publications

Contingency nature of Helicobacter bizzozeronii oxygen-insensitive NAD(P)H-nitroreductase (HBZC1_00960) and its role in metronidazole resistance

Contingency nature of Helicobacter bizzozeronii oxygen-insensitive NAD(P)H-nitroreductase (HBZC1_00960) and its role in metronidazole resistance

Helicobacter heilmannii sensu lato: An overview of the infection in humans

Prevalence of Coinfection with Gastric Non‐Helicobacter pylori Helicobacter (NHPH) Species in Helicobacter pylori‐infected Patients Suffering from Gastric Disease in Beijing, China

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