Culture of agar-embedded one and two cell bovine embryos and embryos produced by nuclear transfer in the sheep and rabbit oviduct

Westhusin, Mark; Slapak, J.R.; Fuller, D.T.; Kraemer, D.C.

doi:10.1016/0093-691x(89)90679-1

Cited by 14 publications

(2 citation statements)

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“…There are two methods for reconstructing the embryonic zona pellucida, one using agarose capsules (20) and other using sodium alginate capsules (21). Both methods have disadvantages, in that they have complicated process of production or inhibition of embryonic development (22, 23). In this present study, it was observed that a 6-hour embryonic in-droplet culture produced no effects on the subsequent development of the embryos.…”

Section: Resultsmentioning

confidence: 99%

Establishment of a novel method for the production of chimeric mouse embryos using oil droplets

Imai

Tsuda

Iwamori

et al. 2019

Preprint

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Section: Resultsmentioning

confidence: 99%

Establishment of a novel method for the production of chimeric mouse embryos using oil droplets

Imai

Tsuda

Iwamori

et al. 2019

Preprint

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“…Embedding in agar has served to improve the in vivo development of micromanipulated bovine embryos (Westhusin et al, 1989) and the in vitro development of frozen-thawed demi-embryos (Niemann et al, 1986). However, gelification of agar is temperature-dependent, and the critical temperature of the phase change is very close to the lethal temperature for embryos.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Development of Bovine Embryos Encapsulated in Sodium Alginate

Yániz

Santolaria²,

López‐Gatius

2002

Journal of Veterinary Medicine Series A

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The objective of this study was to evaluate the in vitro development of bovine embryos encapsulated in alginate. Day-4 embryos produced in vitro (n = 110) were encapsulated with 1.5% sodium alginate and co-cultured with oviduct cells. Unencapsulated embryos (n = 106) were used as controls. In vitro development rate to the blastocyst stage at Day 7 was similar between encapsulated, 42.7%, (47/110) and control. 34% (36/106). embryos. Although encapsulated embryos were able to hatch on Day 9, they did so in a lower proportion than controls (P < 0.05). In conclusion, alginate encapsulation of bovine embryos does not disturb the in vitro development up to the blastocyst stage but significantly reduces the hatching process.

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