Culture of cryopreserved ovarian tissue: state of the art in 2008

Fabbri, Raffaella; Pasquinelli, Gianandrea; Keane, D.; Mozzanega, Bruno; Magnani, Valentina; Tamburini, Federica; Venturoli, Stefano

doi:10.1016/j.fertnstert.2009.03.109

Cited by 23 publications

(9 citation statements)

References 50 publications

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“…Examples of these investigations include histological examination, transmission electron microscopy, immunohistochemistry, terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nick end-labeling (TUNEL) assay, polymerase chain reaction (PCR), estradiol and progesterone production in vitro , and xenografting [76-83]. After freezing, the ovarian tissue is stored in liquid nitrogen at −196 °C for up to 10 years.…”

Section: Resultsmentioning

confidence: 99%

New advances in ovarian autotransplantation to restore fertility in cancer patients

Salama

Woodruff

2015

Cancer Metastasis Rev

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Human ovary autotransplantation is a promising option for fertility preservation of young women and girls undergoing gonadotoxic treatments for cancer or some autoimmune diseases. Although experimental, it resulted in at least 42 healthy babies worldwide. According to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a systematic literature review was performed for all relevant full-text articles published in English from 1 January 2000 to 01 October 2015 in PubMed to explore the latest clinical and research advances of human ovary autotransplantation. Human ovary autotransplantation involves ovarian tissue extraction, freezing/thawing, and transplantation back into the same patient. Three major forms of human ovary autotransplantation exist including (a) transplantation of cortical ovarian tissue, (b) transplantation of whole ovary, and (c) transplantation of ovarian follicles (artificial ovary). According to the recent guidelines, human ovary autotransplantation is still considered experimental; however, it has unique advantages in comparison to other options of female fertility preservation. Human ovary autotransplantation (i) does not need prior ovarian stimulation, (ii) allows immediate initiation of cancer therapy, (iii) can restore both endocrine and reproductive ovarian functions, and (iv) may be the only fertility preservation option suitable for prepubertal girls or for young women with estrogen-sensitive malignancies. As any other fertility preservation option, human ovary autotransplantation has both advantages and disadvantages and may not be feasible for all cases. The major challenges facing this option are how to avoid the risk of reintroducing malignant cells and how to prolong the lifespan of ovarian transplant as well as how to improve artificial ovary results.

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Section: Resultsmentioning

confidence: 99%

New advances in ovarian autotransplantation to restore fertility in cancer patients

Salama

Woodruff

2015

Cancer Metastasis Rev

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“…We therefore assessed the steroidogenic capacity of the tissue, which in turn allowed us to infer the quality of its function in terms of hormone production. Ovarian tissue culture has been described by some authors as a way of assessing the functional capacity of ovarian tissue or of obtaining follicles in more advanced developmental stages for reproductive purposes 19,20. Sadeu et al21 reported maintaining the viability of and inducing partial follicle maturation in human fetal ovarian cultures for up to 63 days.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation

Campos

Silva

Carvalho

et al. 2011

Clinics

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OBJECTIVE:To determine the effect of storage duration on cryopreserved ovarian tissue using fresh and frozen-thawed samples.METHODS:Seventeen fertile patients underwent an ovarian biopsy during elective laparoscopic tubal ligation. The tissue sample was divided into three parts: one part was processed fresh (FG), and two were slowly frozen, cryopreserved for 30 (G30) or 180 days (G180), thawed and analyzed. Follicular density, follicular viability, and steroidogenic capacity were assessed.RESULTS:We observed no differences between the groups in follicular density, which was assessed in hematoxylin and eosin–stained tissue sections. A heterogeneous follicular distribution was observed in the parenchyma, with a mean density of 361.3±255.4, 454.9±676.3, and 296.8±269.0 follicles/mm3 for FG, G30 and G180, respectively (p = 0.46). Follicular viability was greater in FG (93.4%) when compared with the cryopreserved tissues (70.8% for G30 (p<0.001) and 78.4% for G180 (p<0.001)), with no difference in viability between the frozen samples (p>0.05). The steroidogenic capacity of the tissue was not significantly reduced following cryopreservation.CONCLUSION:The slow freezing procedures used for ovarian cryopreservation are capable of preserving follicular viability and maintaining the steroidogenic capacity of the tissue despite a roughly 30% decrease in follicular viability. Furthermore, short-term storage of ovarian tissue does not appear to compromise follicle integrity.

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“…One of the major issues regarding ovarian tissue transplantation is the risk of transmission of cancer cells that may have infiltrated the ovarian tissue before the cryopreservation procedure. In these cases, alternatives include in vitro growth of primordial follicles but unfortunately ovarian tissue culture system is not yet available for human application [12,13]. Furthermore, ovarian tissue cryopreservation preserves the primordial and primary follicles, but not the immature oocytes within the antral follicles that do not survive the procedure.…”

Section: Introductionmentioning

confidence: 99%

Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation

Fasano

Moffa

Dechene

et al. 2011

Reprod Biol Endocrinol

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BackgroundIn the past few years, cryopreservation of ovarian tissue has become an established procedure proposed in many centers around the world and transplantation has successfully resulted in full-term pregnancies and deliveries in human. This prospective study aims to evaluate the feasibility of vitrifying in vitro matured oocytes (IVM) isolated at the time of ovarian tissue cryopreservation to improve the efficiency of fertility preservation programs.MethodsOocyte-cumulus complexes were retrieved from freshly collected ovarian cortex by aspirating antral follicular fluid, and were matured in vitro for 24-48 h prior to vitrification. Oocytes were matured in an IVM commercial medium (Copper Surgical, USA) supplemented with 75 mIU/ml FSH and 75 mIU/ml LH and vitrified using a commercial vitrification kit (Irvine Scientific, California) in high security vitrification straws (CryoBioSystem, France). Oocyte collection and IVM rates were evaluated according to the age, the cycle period and the amount of tissue collected.ResultsImmature oocyte retrieval from ovarian tissue was carried out in 57 patients between 8 and 35 years of age, undergoing ovarian tissue cryopreservation. A total of 266 oocytes were isolated, 28 of them were degenerated, 200 were at germinal vesicle stage (GV), 35 were in metaphase I (MI) and 3 displayed a visible polar body (MII). The number of oocytes collected was positively correlated with the amount of tissue cryopreserved (p < 0.001) and negatively correlated with the age of the patients (p = 0.005). Oocytes were obtained regardless of menstrual cycle period or contraception. A total maturation rate of 31% was achieved, leading to the vitrification of at least one mature oocyte for half of the cohort.ConclusionsThe study showed that a significant number of immature oocytes can be collected from excised ovarian tissue whatever the menstrual cycle phases and the age of the patients, even for prepubertal girls.

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Culture of cryopreserved ovarian tissue: state of the art in 2008

Cited by 23 publications

References 50 publications

New advances in ovarian autotransplantation to restore fertility in cancer patients

New advances in ovarian autotransplantation to restore fertility in cancer patients

Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation

Vitrification of in vitro matured oocytes collected from antral follicles at the time of ovarian tissue cryopreservation

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