Culture of dermal fibroblasts and protein adsorption on block conetworks of poly(butyl methacrylate-block-(2,3 propandiol-1-methacrylate-stat-ethandiol dimethacrylate))

Sun, Yun; Collett, John; Fullwood, Nigel J.; Neil, S. Mac; Rimmer, Stephen

doi:10.1016/j.biomaterials.2006.09.024

Cited by 48 publications

(60 citation statements)

References 37 publications

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“…However, it is likely that other factors are also important and further work would be needed before the role of charge could be confirmed. Our previous work showed that human fibroblasts did not adhere to poly(GMA-co-EGDMA) hydrogels but they did adhere to the same networks modified with LMA, poly(GMA-co-EGDMA-co-LMA) [5]. In this work we studied one set of aminated materials based on poly(GMA-co-EGDMA) and two similar materials (with different EWCs) based on poly(GMA-co-EGDMA-co-LMA).…”

Section: Discussionmentioning

confidence: 99%

“…In particular, their non-adhesive nature allows for modification with molecules that can guide cell growth [1] and their swollen state allows diffusion and release of essential biomolecules throughout the construct [2]. In this connection we previously showed that mammalian cells can adhere and proliferate on amphiphilic conetworks [3][4][5]. In that work, the polymer conetworks, formed from copolymerization of preformed oligomers [6] and low molecular weight monomer, phase separated from the reaction medium during synthesis to give porous structures and cell adhesion and proliferation was dependant both on overall polymer composition and on morphology.…”

Section: Epithelialization Of Hydrogels Achieved By Amine Functionalimentioning

confidence: 99%

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Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells

et al. 2007

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(2007). Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells. Biomaterials, 28 (35), 5319 -5331. The aim of this study was to develop a hydrogel which would be suitable for corneal cell re-epithelialization when used as a corneal implant. To achieve this, a series of hydrogels were functionalized with primary amines by post-polymerization reactions between amine compounds and glycidyl ether groups attached to the hydrogels. We report a strong correlation between the structure of the amine and the viability of stromal cells and epithelial cells cultured on these hydrogels. Subsequent co-culture of epithelial and stromal cells on the amine modified hydrogels allowed successful expansion of epithelial cells on surfaces functionalised with alkyl α-ω diamines with carbon chain lengths of between 3 and 6. Analysis of variance showed that corneal epithelial cells had a strong preference for surfaces functionalized by the reaction of excess 1,3 diaminopropane with units of glycidyl methacrylate compared to the reaction products of other amines (ammonia; 1,2-diaminoethane; 1,4-diaminobutane or 1,6-diaminohexane). We suggest this approach of amine functionalization combined with stromal/epithelial co-culture offers a promising new approach to achieving a secure corneal epithelium. Copyright and re-use policy AUTHOR DECLARATIONWe the undersigned declare that this manuscript is original, has not been published before and is not currently being considered for publication elsewhere.We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.We confirm that the manuscript has been read and approved by all named authors and that there are no other persons who satisfied the criteria for authorship but are not listed. We further confirm that the order of authors listed in the manuscript has been approved by all of us.We confirm that we have given due consideration to the protection of intellectual property associated with this work and that there are no impediments to publication, including the timing of publication, with respect to intellectual property. In so doing we confirm that we have followed the regulations of our institutions concerning intellectual property.We further confirm that any aspect of the work covered in this manuscript that has involved either experimental animals or human patients has been conducted with the ethical approval of all relevant bodies and that such approvals are acknowledged within the manuscript.We understand that the Corresponding Author is the sole contact for the Editorial process (including Editorial Manager and direct communications with the office). He is responsible for communicating with the other authors about progress, submissions of revisions and final approval of proofs. We confirm that we have provided a current, correct email address which is accessible by the Corresponding Author and which ...

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Section: Discussionmentioning

confidence: 99%

Section: Epithelialization Of Hydrogels Achieved By Amine Functionalimentioning

confidence: 99%

Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells

et al. 2007

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“… SEM images of ( A ) electrospun PLLA:PLGA 85:15 scaffolds, ( B ) SEM micrograph of keratinocytes and fibroblasts cocultured on a PLLA:PLGA 75:25 scaffold for 7 days (Blackwood et al, 2008), ( C ) SEM micrograph of human dermal fibroblasts cultured on GMMA polymer for 4 days (Sun et al, 2007b), ( D ) SEM of human osteoblast‐like (HOB) cell cultured on hydroxyapatite (HA) filled poly(methylmethacrylate) (PMMA). The cell has normal osteoblast morphology.…”

Section: Electron Microscopymentioning

confidence: 99%

A comparison of imaging methodologies for 3D tissue engineering

Smith

Smallwood

MacNeil

2010

Microscopy Res & Technique

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Imaging of cells in two dimensions is routinely performed within cell biology and tissue engineering laboratories. When biology moves into three dimensions imaging becomes more challenging, especially when multiple cell types are used. This review compares imaging techniques used regularly in our laboratory in the culture of cells in both two and three dimensions. The techniques reviewed include phase contrast microscopy, fluorescent microscopy, confocal laser scanning microscopy, electron microscopy, and optical coherence tomography. We compare these techniques to the current "gold standard" for imaging three-dimensional tissue engineered constructs, histology.

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“…Amphiphilic conetwork (APCN), a novel polymeric material consisting of hydrophilic and hydrophobic polymer segments through covalently interconnection, has become one of the forefronts in biomaterial research . The extraordinary composition of the networks exhibit unique properties, that is, swelling dependence on solvent, phase separation at nanoscale, mechanical stability, and excellent biocompatibility, which renders them suitable for versatile applications in controlled drug delivery, extended‐wear contact lenses, and biochemical sensors …”

Section: Introductionmentioning

confidence: 99%

Biocompatible amphiphilic conetwork based on crosslinked star copolymers: A potential drug carrier

Wang

Qin

et al. 2015

J. Polym. Sci. Part A: Polym. Chem.

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A series of amphiphilic conetworks (APCNs) is synthesized through crosslinking of well-defined tri-arm star diblock copolymers via atom transfer radical polymerization. A new three-arm initiator is synthesized to initiate the polymerization of 2-hydroxyethyl methacrylate (HEMA) via "core-first" method. The resulting star HEMA homopolymers with welldefined molecular weight and narrow polydispersity are used as macroinitiator to incorporate allyl methacrylate to get the star diblock copolymers. Then, the precursors with allyl pendant groups are fully crosslinked with polyhydrosiloxanes through hydrosilylation. The so-prepared APCNs exhibit unique properties of microphase separation of hydrophilic (HI) and hydrophobic (HO) phases with small channel size, a variable swelling capacity, excellent biocompatibility, and outstanding mechanical strength (2 6 0.5 MPa). The properties of APCNs depend on the ratio of HI to HO, which can be regulated via precise synthesis of the star diblock copolymers. The APCNs show well-controlled drug release to choline theophyllinate, suggesting a promising intelligent drug carrier for controlled release.

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Culture of dermal fibroblasts and protein adsorption on block conetworks of poly(butyl methacrylate-block-(2,3 propandiol-1-methacrylate-stat-ethandiol dimethacrylate))

Cited by 48 publications

References 37 publications

Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells

Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells

A comparison of imaging methodologies for 3D tissue engineering

Biocompatible amphiphilic conetwork based on crosslinked star copolymers: A potential drug carrier

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