Culture of Early‐Stage Bovine Embryos inside Day‐13 and Day‐14 Precultured Trophoblastic Vesicles <sup>a</sup>

Pool, S.H.; Rorie, R.W.; Pendleton, R.J.; Menino, Alfred R.; Godke, R.A.

doi:10.1111/j.1749-6632.1988.tb22277.x

Cited by 19 publications

(5 citation statements)

References 18 publications

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“…Interestingly, while the number of cells increased in quiescent embryos from 60 to 120, this number remained unchanged in TVs, an indication that paracrine signals from the ICM regulate TB cell proliferation. Likewise, co-culture of single bovine blastocysts with TVs improved their development in vitro, further evidence of the existence of paracrine signals between the TB and ICM (Camous et al 1984, Pool et al 1988, Mori et al 2012.…”

Section: Experimental Evidence From Trophoblastic Vesiclesmentioning

confidence: 93%

Preimplantation development in ungulates: a ‘ménage à quatre’ scenario

2020

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In ungulates, early embryonic development differs dramatically from that of mice and humans and is characterized by an extended period of pre- and peri-implantation development in utero. After hatching from the zona pellucida, the ungulate blastocyst will stay free in the uterus for many days before implanting within the uterine wall. During this protracted peri-implantation period, an intimate dialog between the embryo and the uterus is established through a complex series of paracrine signals. The blastocyst elongates, leading to extreme growth of extra-embryonic tissues, and at the same time, the inner cell mass moves up into the trophoblast and evolves into the embryonic disc, which is directly exposed to molecules present in the uterine fluids. In the peri-implantation period, uterine glands secrete a wide range of molecules, including enzymes, growth factors, adhesion proteins, cytokines, hormones, and nutrients like amino and fatty acids, which are collectively referred to as histotroph. The identification, role, and effects of these secretions on the biology of the conceptus are still being described; however, the studies that have been conducted to date have demonstrated that histotroph is essential for embryonic development and serves a critical function during the pre- and peri implantation periods. Here, we present an overview of current knowledge on the molecular dialogue among embryonic, extraembryonic, and maternal tissues prior to implantation. Taken together, the body of work described here demonstrates the extent to which this dialog enables the coordination of the development of the conceptus with respect to the establishment of embryonic and extra-embryonic tissues as well as in preparation for implantation.

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Section: Experimental Evidence From Trophoblastic Vesiclesmentioning

confidence: 93%

Preimplantation development in ungulates: a ‘ménage à quatre’ scenario

2020

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“…A typical coculture system involves placing embryos either in a cell's suspension or on top of cells in a monolayer. Examples of such systems include bovine oviductal epithelial cells (BOEC) (Fukui & Ono 1988, Lu et al 1988, Eyestone & First 1989, Wiemer et al 1991, granulosa cells (Goto et al 1988, Tan & Lu 1990, Wiemer et al 1991, cumulus cells (Goto et al 1990, Kajihara et al 1990, Mermillod et al 1992, trophoblastic vesicles (Camous et al 1984, Heyman et al 1987, uterine endometrial cells/fibroblasts (Wiemer et al 1987, Pool et al 1988, Kajihara et al 1991 and amniotic sac cells (Aoyagi et al 1989).…”

Section: Coculture Systemsmentioning

confidence: 99%

Growth of Preimplatation Bovine Embryos

Lonergan¹

1994

Acta Vet Scand

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“…Of the 211 fertilized and unfertilized ova collected, 162 were at the two-to eight-cell developmental stage and were assessed as being of good or excellent quality based on caprine embryo morphology and quality grade scores (1, excellent; 2, good; 3, fair; and 4, poor) as previously outlined by Pendelton et al (1986b). The harvested embryos were then placed into a modified PBS holding medium with 10% FBS as described by Pool et al (1988) and held at 37°C until allotment to treatments.…”

Section: E M B R Y O Collectionmentioning

confidence: 99%

“…The most frequently used co-culture systems are monolayers of feeder cells (Cole and Paul, 1965), uterine fibroblasts (Kuzan and Wright, 1982;Voelkel et al, 19851, or more recently fetal uterine fibroblasts (Wiemer et al, 1987(Wiemer et al, , 1988a and oviductal cell preparations (Gandolfi and Moor, 1987;Rexroad and Powell, 1988). Another procedure involves the microsurgical sectioning of later-stage conceptus trophectoderm to produce trophoblastic vesicles (Camous et al, 1984;Heyman et al, 1987;Pool et al, 1988) for co-culture with earlier-stage embryos. In spite of the uncharacterized nature of such culture systems, they represent the most effective means of obtaining development of farm animal embryos in vitro.…”

Section: Introductionmentioning

confidence: 99%

Culturing two‐ to eight‐cell caprine embryos using domestic chicken eggs

Blakewood

Pool

Prichard

et al. 1990

Molecular Reproduction Devel

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Early-stage caprine embryos were placed in the chick embryo amnion to determine if this culture method would support the development of embryos from a farm animal species. Following superovulation and natural mating, two- to eight-cell embryos were surgically collected from crossbred donor goats. Embryos were allotted to in vitro culture treatments across two different experiments (EXP). In EXP-I, embryos allotted to Treatment A (control) were cultured in Ham's F-10 with 10% fetal calf serum and 1% antibiotic-antimycotic (HF-10). Embryos in Treatment B were placed on a bovine fetal uterine fibroblast monolayer in HF-10, embryos allotted to Treatment C were agarose embedded and injected into the amniotic cavity of a day-4 chick embryo and those placed in Treatment D were co-cultured in HF-10 with day-15 caprine trophoblastic vesicles. In EXP II Treatments A, B, and C were the same; however Treatment D was omitted. EXP-I and EXP-II also differed in that chick embryo co-culture was for 72 hr in EXP-I but was extended to 96 hours in EXP-II. Additionally, the monolayer co-culture was limited to 96 hr in EXP-II; whereas, embryos in EXP-I remained on monolayer culture for 96 hr plus an additional 72 hr for subsequent embryo evaluation. Results indicate that the amniotic cavity of the developing chick embryo enhanced the development of two- to eight-cell caprine embryos through to hatching blastocysts when compared with that of the control medium alone.(ABSTRACT TRUNCATED AT 250 WORDS)

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Culture of Early‐Stage Bovine Embryos inside Day‐13 and Day‐14 Precultured Trophoblastic Vesicles ^a

Cited by 19 publications

References 18 publications

Preimplantation development in ungulates: a ‘ménage à quatre’ scenario

Preimplantation development in ungulates: a ‘ménage à quatre’ scenario

Growth of Preimplatation Bovine Embryos

Culturing two‐ to eight‐cell caprine embryos using domestic chicken eggs

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Culture of Early‐Stage Bovine Embryos inside Day‐13 and Day‐14 Precultured Trophoblastic Vesicles a

Cited by 19 publications

References 18 publications

Preimplantation development in ungulates: a ‘ménage à quatre’ scenario

Preimplantation development in ungulates: a ‘ménage à quatre’ scenario

Growth of Preimplatation Bovine Embryos

Culturing two‐ to eight‐cell caprine embryos using domestic chicken eggs

Contact Info

Product

Resources

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Culture of Early‐Stage Bovine Embryos inside Day‐13 and Day‐14 Precultured Trophoblastic Vesicles ^a