Culture of goat preantral follicles in situ associated with mesenchymal stem cell from bone marrow

Abstract

SummaryThis study aims to develop an in vitro co-culture system of in situ goat preantral follicles with bone marrow-derived mesenchymal stem cells (BM-MSC), evaluating the influence of these cells on follicular growth, rate of activation and morphologically normal follicles. Fragments of ovarian cortex were cultured for 1 or 7 days in the presence of BM-MSC (BM-MSC+) and absence of BM-MSC (BM-MSC−). Histological sections of the fragments were analysed and data were obtained regarding morphological classification, survival rate of morphologically normal follicles and rate of follicular activation. Culture medium on days 1 and 7 was also sampled for nitrite concentration and reduced glutathione activity. There was a reduction (P < 0.05) in the percentage of morphologically normal follicles in the BM-MSC+ compared with the fresh control only on the seventh day of culture. When comparing treatments, on the seventh day of culture, a higher rate of morphologically normal preantral follicles was observed in BM-MSC+ (P < 0.05). In both treatments, primordial and developing follicle rates were similar to the fresh control (P > 0.05). When comparing treatments with each other, as well as with the fresh control, no differences were observed in follicular diameter (P > 0.05) or nitrite concentration (P > 0.05). The concentration of reduced glutathione was lower on the seventh day of co-culture in both treatments (P < 0.05). In conclusion, co-culture had no influence on follicular or oocyte development. However, it was critical to maintain the survival of preantral follicles during 7 days of culture.

Comprehensive assessment of goat adipose tissue-derived mesenchymal stem cells cultured in different media

“…In a separate investigation comparing four BCMs, ADSCs exhibited enhanced proliferation when MEM and DMEM-LG were employed, with no notable alterations in morphology and viability during subsequent passages 27 . However, the goat adipose-derived MSCs (gADSCs) have been cultivated in various basal media, including MEM 28 – 32 , DMEM-LG 20 , 33 – 42 , Dulbecco’s MEM/ Nutrient Mixture F12 (DMEM/F12) 43 – 52 and DMEM-HG 53 , which media best supports gADSCs cell growth and proliferation remains elusive. The optimal selection of BCM can be contingent on the type and quantity of MSCs under cultivation.…”

Evaluation of the Effects of Human Bone Marrow Mesenchymal Stem Cells Conditioned Medium on Growth and Maturation of Mouse Ovarian Follicle after Vitrification

“…MSCs are able to establish a regenerative microenvironment through the secretion of bioactive factors, trophic and antiapoptotic molecules. In addition, according to the interrelation between MSCs and co-cultured cells, a proper source of trophic paracrine factors could be provided using MSCs, as a feeder cell [Zhang et al, 2017;Hosseini et al, 2019;Joseph et al, 2020;Neves et al, 2020].…”