Culture of human osteoblasts on demineralised human bone. Possible means of graft enhancement

Nolan, Patrick; Nicholas, R. M.; Mulholland, B. J.; Mollan, R. A. B.; Wilson, David J.

doi:10.1302/0301-620x.74b2.1312095

Cited by 32 publications

(16 citation statements)

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“…[11][12][13] Accordingly, osteoblastic cell behavior on biomaterials was shown to be influenced by the composition, porosity, nature of the surface, and physical characteristics of the biomaterials. 14,15 Although several neonatal rat calvaria cells, 14,16,17 osteoblastic cell lines, 18,19 marrow stromal cells, 20,21 human bone cells, 15,[22][23][24] and marrow stromal cells 25 were reported to adhere and grow on biomaterials in vitro, it remains uncertain whether human osteoblastic cells can differentiate normally in longterm culture on biomaterials. In this study, we have evaluated whether human trabecular (HT) osteoblastic cells proliferate and differentiate on dense hydroxyapatite in comparison with standard plastic culture.…”

Section: Introductionmentioning

confidence: 99%

Proliferation and differentiation of human trabecular osteoblastic cells on hydroxyapatite

Hott

Noël

Bernache-Assolant

et al. 1997

J. Biomed. Mater. Res.

118

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In order to evaluate whether human osteoblastic cells differentiate normally on hydroxyapatite, we have compared the adhesion, proliferation, and differentiation of human trabecular (HT) osteoblastic cells on synthetic-dense hydroxyapatite and on standard plastic culture. We show here that initial HT cell attachment was 4-fold lower on hydroxyapatite than on plastic after 4 h of culture, and that normal cell attachment on hydroxyapatite was restored after 18 h of culture. HT cell proliferation was similar on the two substrates at 2-8 days of culture, but was lower on hydroxyapatite compared to plastic after 15 and 28 days of culture, as evaluated by DNA synthesis or cell number. HT cells cultured on both substrates produced an abundant extracellular matrix which immunostained for Type I collagen. The levels of carboxyterminal propeptide of Type I procollagen (P1CP) in the medium were lower in HT cell cultures on hydroxyapatite than on plastic. In addition, (3H)-proline incorporation into matrix proteins and the mean thickness of matrix layers were 52% and 26% lower, respectively, on hydroxyapatite compared to plastic after 4 weeks of culture, indicating that the total collagenous matrix synthesized by HT cells was lower on hydroxyapatite. However, (3H)-proline and calcium uptake expressed per cell was higher on hydroxyapatite than on plastic. The results show that human osteoblastic cells attach, proliferate, and differentiate on dense hydroxyapatite with a sequence similar to that of plastic. However, the growth of human osteoblastic cells is lower on hydroxyapatite in long-term culture, which results in a reduced amount of extracellular matrix, although matrix production per cell may be increased.

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Section: Introductionmentioning

confidence: 99%

Proliferation and differentiation of human trabecular osteoblastic cells on hydroxyapatite

Hott

Noël

Bernache-Assolant

et al. 1997

J. Biomed. Mater. Res.

118

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“…The osteoblasts are thus the focal point for development and assessment of novel therapeutic agents for osteoporosis. Studying mature human osteoblasts in vitro most often involves collection of bone fragments from orthopedic surgery and subsequently cultivation of human trabecular bone cells (35,42,46). The relative ease of human osteoblast-like cell (HOb) isolation from an abundant clinical source, which would otherwise be disposed, makes them an accessible primary cell model for studying cell physiology in human cell panels.…”

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confidence: 99%

Systematic assessment of the human osteoblast transcriptome in resting and induced primary cells

Grundberg

Brändström

Lam

et al. 2008

Physiological Genomics

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Osteoblasts are key players in bone remodeling. The accessibility of human primary osteoblast-like cells (HObs) from bone explants makes them a lucrative model for studying molecular physiology of bone turnover, for discovering novel anabolic therapeutics, and for mesenchymal cell biology in general. Relatively little is known about resting and dynamic expression profiles of HObs, and to date no studies have been conducted to systematically assess the osteoblast transcriptome. The aim of this study was to characterize HObs and investigate signaling cascades and gene networks with genomewide expression profiling in resting and bone morphogenic protein (BMP)-2- and dexamethasone-induced cells. In addition, we compared HOb gene expression with publicly available samples from the Gene Expression Omnibus. Our data show a vast number of genes and networks expressed predominantly in HObs compared with closely related cells such as fibroblasts or chondrocytes. For instance, genes in the insulin-like growth factor (IGF) signaling pathway were enriched in HObs ( P = 0.003) and included the binding proteins (IGFBP-1, -2, -5) and IGF-II and its receptor. Another HOb-specific expression pattern included leptin and its receptor ( P < 10−8). Furthermore, after stimulation of HObs with BMP-2 or dexamethasone, the expression of several interesting genes and pathways was observed. For instance, our data support the role of peripheral leptin signaling in bone cell function. In conclusion, we provide the landscape of tissue-specific and dynamic gene expression in HObs. This resource will allow utilization of osteoblasts as a model to study specific gene networks and gene families related to human bone physiology and diseases.

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“…The relapse risk increases in patients who undergo a large amount of bone movement with only rigid fixation due to less contact of the bone margins after orthognathic operations [3,4]. Therefore, autogenous, homogeneous (allograft) and heterogeneous bone grafts (xenograft), and alloplastic implants can be used for filling the bone gaps after orthognathic surgery [5].…”

Section: Original Papersmentioning

confidence: 99%

Comparison of Autologous and Heterologous Bone Graft Stability Effects for Filling Maxillary Bone Gap After Le Fort I Osteotomy

Eser¹,

Gencel²,

Gökdoğan³

et al. 2015

Adv Clin Exp Med

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Background. The amount of postoperative maxillary relapse of two different bone graft materials after Le Fort I osteotomy were compared in this study. Objectives. The aim of this study is to compare postoperative maxillary relapse rates using heterologous and autologous graft materials after Le Fort I osteotomy. Material and Methods. A total of 80 patients who had developmental malocclusion were analyzed retrospectively in this study. Twenty nine (36.2%) and 51 (63.8%) patients underwent Le Fort I osteotomy, and Le Fort I and bilateral sagittal split ramus osteotomy (two-jaw surgery), respectively. Forty two (52.5%) maxillary bone gaps were filled with heterologous bone grafts (group A) and 38 (47.5%) were filled with autologous bone grafts (group B) after Le Fort I osteotomy. The cephalometric graphics and measurements were taken before (T1), 1 week after (T2), and 1 year after (T3) the surgery. The results were documented and determined by the Dolphin imaging 10.5 (Dolphin Imaging, Chatsworth, Calif.) computer program for skeletal relapse. Whether or not the relationship between group A's and B's maxillary relapse rates was evaluated in the postoperative period. Results. It was observed that both graft materials have positive effects on maxillary relapse rate in the postoperative period. When the groups are compared to each other, the relapse rates were similar between group A (8.3%) and group B (10.8%) (p > 0.05). Conclusions. Heterologous bone graft material (Osteoplant ® -Flex) is thought to be a good alternative to autologous grafts in decreasing the relapse rates and reducing the morbidity of the donor area of the patients who underwent Le Fort I osteotomy (Adv Clin Exp Med 2015, 24, 2, 341-348).

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Culture of human osteoblasts on demineralised human bone. Possible means of graft enhancement

Cited by 32 publications

References 0 publications

Proliferation and differentiation of human trabecular osteoblastic cells on hydroxyapatite

Proliferation and differentiation of human trabecular osteoblastic cells on hydroxyapatite

Systematic assessment of the human osteoblast transcriptome in resting and induced primary cells

Comparison of Autologous and Heterologous Bone Graft Stability Effects for Filling Maxillary Bone Gap After Le Fort I Osteotomy

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