Culture of human vascular endothelial cells on an RGD-containing synthetic peptide attached to a starch-coated polystyrene surface: comparison with fibronectin-coated tissue grade polystyrene

Holland, James A.; Hersh, L. S.; Bryhan, Marie; Onyiriuka, Emmanuel C.; Ziegler, Linda M.

doi:10.1016/0142-9612(96)00028-2

Cited by 62 publications

(40 citation statements)

References 14 publications

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“…For example, to obtain EC-specific adhesion, covalently bonding synthetic peptides to the biomaterials is required. This binding relies heavily on the receptor-binding domains of cell adhesion proteins [Cheresh, 1987;Massia and Hubbell, 1991;Holland et al, 1996;Alobaid et al, 2006]. No additional surface modification of the electrospun collagen-elastin scaffold was necessary for attachment of hASCs to the scaffold.…”

Section: Discussionmentioning

confidence: 99%

Human Adipose Stem Cells: A Potential Cell Source for Cardiovascular Tissue Engineering

Heydarkhan‐Hagvall¹,

Schenke‐Layland²,

Yang³

et al. 2008

Cells Tissues Organs

110

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Background/Aims: A crucial step in providing clinically relevant applications of cardiovascular tissue engineering involves the identification of a suitable cell source. The objective of this study was to identify the exogenous and endogenous parameters that are critical for the differentiation of human adipose stem cells (hASCs) into cardiovascular cells. Methods: hASCs were isolated from human lipoaspirate samples, analyzed, and subjected to two differentiation protocols. Results: As shown by fluorescence-activated cell sorter (FACS) analysis, a population of hASCs expressed stem cell markers including CXCR4, CD34, c-kit, and ABCG2. Further, FACS and immunofluorescence analysis of hASCs, cultured for 2 weeks in DMEM-20%-FBS, showed the expression of smooth muscle cell (SMC)-specific markers including SM α-actin, basic calponin, h-caldesmon and SM myosin. hASCs, cultured for 2 weeks in endothelial cell growth medium-2 (EGM-2), formed a network of branched tube-like structures positive for CD31, CD144, and von Willebrand factor. The frequency of endothelial cell (EC) marker-expressing cells was passage number-dependent. Moreover, hASCs attached and formed a confluent layer on top of electrospun collagen-elastin scaffolds. Scanning electron microscopy and DAPI staining confirmed the integration of hASCs with the fibers and formation of a cell-matrix network. Conclusion: Our results indicate that hASCs are a potential cell source for cardiovascular tissue engineering; however, the differentiation capacity of hASCs into SMCs and ECs is passage number- and culture condition-dependent.

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Section: Discussionmentioning

confidence: 99%

Human Adipose Stem Cells: A Potential Cell Source for Cardiovascular Tissue Engineering

Heydarkhan‐Hagvall¹,

Schenke‐Layland²,

Yang³

et al. 2008

Cells Tissues Organs

110

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“…One widely used approach involves the conjugation of proteins or peptides containing the sequence Arg-Gly-Asp (RGD), which recruits and binds to integrin receptors on the surfaces of eukaryotic cells. 3,[12][13][14][15][16][17] This is particularly significant because differential integrin binding alters specific cellular behaviors such as differentiation in human unmbilical vein endothelial cells. 5 Conversely, differential integrin expression is known to be an important marker of cell state during angiogenesis and capillary invasion during wound healing.…”

Section: Introductionmentioning

confidence: 99%

Biochemical Functionalization of Polymeric Cell Substrata Can Alter Mechanical Compliance

et al. 2006

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Biochemical functionalization of surfaces is an increasingly utilized mechanism to promote or inhibit adhesion of cells. To promote mammalian cell adhesion, one common functionalization approach is surface conjugation of adhesion peptide sequences such as Arg-Gly-Asp (RGD), a ligand of transmembrane integrin molecules. It is generally assumed that such functionalization does not alter the local mechanical properties of the functionalized surface, as is important to interpretations of macromolecular mechanotransduction in cells. Here, we examine this assumption systematically, through nanomechanical measurement of the nominal elastic modulus of polymer multilayer films of nanoscale thickness, functionalized with RGD through different processing routes. We find that the method of biochemical functionalization can significantly alter mechanical compliance of polymeric substrata such as weak polyelectrolyte multilayers (PEMs), increasingly utilized materials for such studies. In particular, immersed adsorption of intermediate functionalization reagents significantly decreases compliance of the PEMs considered herein, whereas polymer-on-polymer stamping of these same reagents does not alter compliance of weak PEMs. This finding points to the potential unintended alteration of mechanical properties via surface functionalization and also suggests functionalization methods by which chemical and mechanical properties of cell substrata can be controlled independently.

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“…Published work has highlighted a number of biologically active coatings, either from serum-supplemented media [10,11], monocomponent ECM proteins in solution [12][13][14], or through the covalent attachment (grafting) of oligopeptides representing the cell-adhesive peptide region of the ECM protein, such as Arg-Gly-Asp (RGD) [15,16]. Coating surfaces with ECM proteins such as fibronectin, vitronectin, and collagen, provides an 'adhesive interface' and a strong mechanical contact between the scaffold material and the anchorage-dependent cells, whose organisation and production modulates and enhances cell adhesion through transmembrane integrin receptors [8].…”

Section: Introductionmentioning

confidence: 99%

Characterisation of ‘wet’ polymer surfaces for tissue engineering applications: Are flat surfaces a suitable model for complex structures?

Safinia

Blaker

Maquet³

et al. 2005

E-Polymers

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Tissue engineering scaffolds are 3D constructs that simulate the growth environment in vivo. The present work aims to address the question of whether thin films, i.e., flat surfaces, are a suitable model for more complex 3D structures? With this in mind a complete study of the morphology and surface chemistry of poly(D,Llactide) (PDLLA) substrates, fabricated into two different structures, is presented. The polymer structures studied include a 3D, porous, foam-like scaffold prepared by the thermally induced phase separation (TIPS) method and flat polymer thin films made by solvent casting. Based on the maximum bubble point test, a new method to assess the wettability of wet pore wall surfaces inside highly porous 3D structures was developed and tested. The maximum pore diameter determined using the maximum bubble point test for the total wetting liquids was confirmed through image analysis of scanning electron micrographs. The method allows the determination of the contact angle between the wet pore wall and a contacting liquid. The captive bubble method was employed to characterise the wettability of flat polymer films in contact with water. Both structures were further characterised using zeta-(ζ-) potential measurements to assess the surface chemistry of the polymer. The results demonstrate that PDLLA contains acidic functional groups and is hydrophobic. In order to evaluate the sensitivity of the test methods, the polymer surfaces were modified by protein adsorption using fibronectin and collagen. ζ-Potential and wettability measurements show that proteins indeed adsorb on virgin PDLLA surfaces. Protein adsorption causes the wettability of the PDLLA for water to improve. Our results strongly indicate that flat surfaces are not a suitable model for surfaces in complex 3D structures such as highly porous tissue engineering scaffolds. Such scaffolds must be characterised as a 3D system.

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Culture of human vascular endothelial cells on an RGD-containing synthetic peptide attached to a starch-coated polystyrene surface: comparison with fibronectin-coated tissue grade polystyrene

Cited by 62 publications

References 14 publications

Human Adipose Stem Cells: A Potential Cell Source for Cardiovascular Tissue Engineering

Human Adipose Stem Cells: A Potential Cell Source for Cardiovascular Tissue Engineering

Biochemical Functionalization of Polymeric Cell Substrata Can Alter Mechanical Compliance

Characterisation of ‘wet’ polymer surfaces for tissue engineering applications: Are flat surfaces a suitable model for complex structures?

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