Culture of Isolated Glomeruli From Normal and Nephritic Rabbits

Morita, Takashi; Oite, Takashi; Kiaara, Itaru; Yamamoto, Tadashi; Hara, Masanori; Naka, A; Ohno, S

doi:10.1111/j.1440-1827.1980.tb03280.x

Cited by 16 publications

(7 citation statements)

References 13 publications

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“…Podocalyxin and pan cadherin are critical markers for distinguishing the two types of cell. From this point of view, it is interesting to examine whether large-sized cells, which have been reported as podocytes in previous papers, are positive for podocalyxin or pan cadherin (Bernik 1969;Holdworth et al 1978;Morita et al 1980;Mundel et al 1997a;Nørgaard 1987).…”

Section: Discussionmentioning

confidence: 99%

“…The first group are regularly shaped polygonal cells with a cobblestone-like appearance and high rates of replication and constitute the main outgrowths from glomeruli for the first 6 days of culture (Cybulsky et al 1987;Foidart et al 1979;Harper et al 1984;Kreisberg et al 1978;Mendrick et al 1991). The second are irregular and arborized and are often multinucleated cells with long cytoplasmic extensions and a very limited proliferative capacity (Bernik 1969;Hancock and Atkins 1983;Holdworth et al 1978;Morita et al 1980;Nørgaard 1983Nørgaard , 1987Yaoita et al 1995). The latter cells occur as a very small population, constituting no more than 5% of glomerular outgrowths (Yaoita et al 1995) and are presumably derived from podocytes, since they express podocyte-specific markers, e.g., podocalyxin, synaptopodin (Hancock and Atkins 1983;Yaoita et al 1995).…”

Section: Introductionmentioning

confidence: 95%

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Phenotypic modulation of parietal epithelial cells of Bowman's capsule in culture

Yaoita

Kurihara

Sakai

et al. 2001

Cell and Tissue Research

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The origin of cobblestone-like polygonal cells (the most numerous in renal glomerular cell culture) remains controversial; they could be either dedifferentiated podocytes or parietal epithelial cells (PECs) of Bowman's capsule. Poor cellular outgrowth from glomeruli devoid of Bowman's capsule (decapsulated glomeruli) hinders podocytes being obtained without contamination of PECs in culture. Since podocytes are easily damaged during the isolation of glomeruli by the conventional sieving method, we devised a gentle isolation method without forced sieving, resulting in substantial numbers of arborized cells growing out from decapsulated glomeruli. The cells were distinctly different from cobblestone-like polygonal cells in their irregular and often arborized shape and extended long cytoplasmic processes that often crossed over adjacent cells. The arborized cells from decapsulated glomeruli showed intense staining for a podocyte-specific marker, podocalyxin, but no staining for markers specific to PECs (pan cadherin), mesangial cells (Thy-1) or endothelial cells (von Willebrand factor, RECA-1), indicating their podocyte origin. Polygonal cells growing out from encapsulated glomeruli were negative for podocalyxin and positive for pan cadherin at the peripheral cell-cell contact. Thus, the cell population from decapsulated glomeruli is distinctly different from that from encapsulated glomeruli, supporting the idea that polygonal cells originate from PECs, although immunocytochemical markers specific to podocytes in vivo such as WT1, synaptopodin, HSP27 and P-31 antigen were expressed significantly in the polygonal cells. Occasionally, large irregular-shaped cells appeared at the periphery of the outgrowths from encapsulated glomeruli. They were similar in shape to the arborized cells from decapsulated glomeruli but were identical in antigenic properties to cobblestone-like polygonal cells and thus may be named "pseudo-arborized cells". We conclude that PECs in culture modulate their phenotype to resemble podocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Phenotypic modulation of parietal epithelial cells of Bowman's capsule in culture

Yaoita

Kurihara

Sakai

et al. 2001

Cell and Tissue Research

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“…ARBORIZED CELLS In addition to polygonal cells, another cell type has been proposed as podocytes in culture in many species (Bernik, 1969;Hankock and Atkins, 1983;Holdworth et al, 1978;Morita et al, 1980;Nørgaard, 1983Nørgaard, , 1987. The cells are very large, arborized, and often multinucleated, with a limited proliferative capacity.…”

Section: Polygonal Cells and Largementioning

confidence: 98%

Polygonal epithelial cells in glomerular cell culture: Podocyte or parietal epithelial origin?

Yaoita

Yoshida

2002

Microscopy Res & Technique

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Podocytes are unique cells with regard to morphology, their inability to proliferate in situ, and their apparent essential function for glomerular filtration and permselectivity. Upon transfer into culture conditions, polygonal epithelial cells grow out from isolated glomeruli and these cells have been postulated to represent dedifferentiated podocytes, even though they are negative for podocyte-specific markers and undergo cell division. However, it is controversial whether they originate from podocytes or parietal epithelial cells (PECs) of Bowman's capsule, because isolated glomeruli contain more or less Bowman's capsule. In this review, we shall summarize recent progress in the identification of the origin of the polygonal cells and the characterization of podocytes in culture.

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“…Five-to seven-week-old Wistar rats were used, from which mesangial cells were isolated and cultured as described previously [23]. Mesangial cells were identified by the standard method [24,25] and cells at passages 4-7 were used in this study.…”

Section: Mesangial Cell Culturementioning

confidence: 99%

Thy-1 molecule associates with protein tyrosine kinase(s) in rat mesangial cells

Narisawa‐Saito

Yamanashi

Morioka

et al. 1996

Clinical and Experimental Immunology

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SUMMARYGlycophosphatidylinositol (GPI)-linked Thy-1 molecules, well known cell surface markers of murine T cells, are present on the glomerular mesangial cells of the rat kidney. The administration of anti-Thy-1.1 MoAbs 1-22-3 and OX-7 to rats induces severe and mild complement-dependent mesangial proliferative glomerulonephritis, respectively. In order to determine whether protein-tyrosine kinase (PTK) activity is associated with Thy-1 molecules on rat mesangial cell surface, we performed an immune complex kinase assay, using anti-Thy-1 MoAbs 1-22-3 and OX-7, followed by reimmunoprecipitation with anti-phosphotyrosine, anti-fyn, anti-lck and anti-lyn antibodies. Physical association of PTK, p59 fyn and p56/53 lyn with Thy-1 molecules was demonstated in cultured rat mesangial cells. The activities of these kinases detected in MoAb 1-22-3 precipitates were higher than those in MoAb OX-7 precipitates. These results suggest that Thy-1 molecule transduces some signals also in rat mesangial cells.

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Culture of Isolated Glomeruli From Normal and Nephritic Rabbits

Cited by 16 publications

References 13 publications

Phenotypic modulation of parietal epithelial cells of Bowman's capsule in culture

Phenotypic modulation of parietal epithelial cells of Bowman's capsule in culture

Polygonal epithelial cells in glomerular cell culture: Podocyte or parietal epithelial origin?

Thy-1 molecule associates with protein tyrosine kinase(s) in rat mesangial cells

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