Culture optimization for production and characterization of bioflocculant by Aspergillus flavus grown on chicken viscera hydrolysate

Mohammed, J.; Dagang, Wan Rosmiza Zana Wan

doi:10.1007/s11274-019-2696-8

Cited by 13 publications

(6 citation statements)

References 60 publications

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“…The results or characteristics of the bioflocculants in these studies vary, which can be attributed to the origin of the microorganism, the methods used for flocculants recovery, and other factors. In addition, because bioflocculants are produced through microbial fermentation, this process is influenced by several variables, such as the availability and composition of nutrients, physicochemical parameters, and culture conditions, resulting in bioflocculants with varying properties [8]. Therefore, intensive research is required to understand their properties better if microbial flocculants are to be implemented on an industrial scale.…”

Section: Identification and Characterization Of The Microbesmentioning

confidence: 99%

“…are produced through microbial fermentation, this process is influenced by several variables, such as the availability and composition of nutrients, physicochemical parameters, and culture conditions, resulting in bioflocculants with varying properties [8]. Therefore, intensive research is required to understand their properties better if microbial flocculants are to be implemented on an industrial scale.…”

Section: Characterization Of the Produced Bioflocculantmentioning

confidence: 99%

“…The results from the ANOVA are shown in Table 2. According to [8] (2019), a p-value of less than 0.05 indicates statistical significance for the analyzed model or component. The current model had a significance level of 0.0281, indicating its significance.…”

Section: Zeta Potentialmentioning

confidence: 99%

“…Many microorganisms such as fungi, algae and bacteria have shown the ability to produce bioflocculants [3] that are produced through microbial fermentation. This process is affected by various factors, including nutrient availability and composition, physicochemical parameters, and culture conditions [8]. The polymeric characterization of bioflocculants varies in terms of their composition of proteins, polysaccharides, polyamino acids, and sugars, with the polysaccharides and proteins receiving the most attention, as they constitute the most significant proportion of the flocculants and consequently influence their flocculation activity [4,9].…”

Section: Introductionmentioning

confidence: 99%

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Bioflocculant Producing Bacillus megaterium from Poultry Slaughterhouse Wastewater: Elucidation of Flocculation Efficacy and Mechanism

Mukandi,

Basitere,

Ntwampe

et al. 2024

Applied Sciences

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The study focused on isolating bioflocculant-producing microorganisms from poultry slaughterhouse wastewater (PSW). Microorganisms (n = 20) were isolated, and the D2 isolate, identified as Bacillus megaterium using 16S rDNA and RpoD (sigma 70), had maximum flocculation activity. Furthermore, characteristics of the bioflocculant produced by B. megaterium were determined, and the optimum storage conditions, including the flocculation mechanism, were identified. The bioflocculant was composed mainly of polysaccharides and proteins and was better stored frozen in a crude form. Furthermore, the flocculation efficacy was assessed using response surface methodology at pH 4 (min) and 9 (max), bioflocculant dosage of 1% (min) and 3% (max, v/v), indicating pH 6.5 and dosage of 2% (v/v) as optimum flocculation conditions for floc formation under ambient temperature. These results were further confirmed with microscopy assessments with zeta potential measurements confirming that the bioflocculant was ionic, albeit charge neutralization was not the primary mechanism for floc agglomeration. Hydrogen bonding was predominant, indicative of a neutralization-bridging mechanism, an assertion also based on the functional groups prevalent in the isolate-B. megaterium. The results obtained indicate that bioflocculants can be used to treat isolates that are sourced from wastewater.

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Section: Identification and Characterization Of The Microbesmentioning

confidence: 99%

Section: Characterization Of the Produced Bioflocculantmentioning

confidence: 99%

Section: Zeta Potentialmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioflocculant Producing Bacillus megaterium from Poultry Slaughterhouse Wastewater: Elucidation of Flocculation Efficacy and Mechanism

Mukandi,

Basitere,

Ntwampe

et al. 2024

Applied Sciences

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“…However, the flocculation rate begins to decline at 120 th hour downward. The decline in bioflocculant synthesis as indicated by the flocculation rate is attributable to nutrient depletion in the production broth, depletion of the oxygen accessible to the microorganism and accumulation of the toxic wastes of the microbial metabolism within the culture broth (Mohammed and Dagang, 2019c). The potentials of the exoenzymes to bring about deflocculation through depolymerisation of the biopolymers (the bioflocculant) into degradable monomeric units after depletion of principal nutrient source in the culture broth to be used as nutrient and energy source for growth was also explained by Aljuboori et al (2013).…”

Section: Effect Of Ph On Bioflocculant Synthesismentioning

confidence: 99%

Production of Bioflocculant through Fermentation of Spoilt Orange Juice with Bacillus spp Isolated from Sediment of Local Clay Pot

Mohammed¹,

Majiya²,

Adisa³

et al. 2023

jasem

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The biodegradable and non-toxic Bioflocculants have attracted considerable interest as alternative to non-biodegradable chemical flocculants. However, the cost of fermentation media for bioflocculant production and low flocculation efficiency is a major challenge. In this study, we grow Bacillus spp isolated from sediment of local clay pot on spoilt orange juice to produce bioflocculant. The culture supernatant of the bacilli grown on spoilt orange juice were screen for bioflocculant production using Jar test method and Kaolin clay suspension as model wastewater. The effect of pH and temperature on the bioflocculant production were also studied. The effect of cations on bioflocculation rate of the bioflocculant produced were tested via hybridization of the bioflocculant with the cations. The bioflocculation efficiency of the bioflocculant on sample wastewaters were also determined. The bacilli isolated initially had flocculation rate between 31.1±1.6 % to 76.4±1.2% when cultured on screening media. Bioflocculation rate peaked to 77.9% at 40 oC while the lowest flocculation rate (55.41%) was obtained at 25 oC. The optimum bioflocculant production (about 78%) was recorded at pH 6 – 7 while the highest bioflocculant production (84.2%) was achieved at 96th hour of incubation. Na+ and Fe3+ had serious inhibitory effect on the bioflocculant while K+, Ca2+, Mg2+ and Al3+ had less or no effect on the bioflocculant. The bioflocculant had up to 77.7% and 64.5 % on Kitchen wastewater and aquaculture wastewater. This study reveals the potential for utilization of spoilt orange juice as fermentable substrate for bioflocculant production especially if the fermentation conditions and flocculation parameters are well optimized.

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Microbial Removal of Arsenic: An Update

Mohammed,

Yahaya,

Aliyu

et al. 2024

Emerging Contaminants and Associated Treatment Technologies

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Culture optimization for production and characterization of bioflocculant by Aspergillus flavus grown on chicken viscera hydrolysate

Cited by 13 publications

References 60 publications

Bioflocculant Producing Bacillus megaterium from Poultry Slaughterhouse Wastewater: Elucidation of Flocculation Efficacy and Mechanism

Bioflocculant Producing Bacillus megaterium from Poultry Slaughterhouse Wastewater: Elucidation of Flocculation Efficacy and Mechanism

Production of Bioflocculant through Fermentation of Spoilt Orange Juice with Bacillus spp Isolated from Sediment of Local Clay Pot

Microbial Removal of Arsenic: An Update

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