Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides

Huang, Xiangfeng; Liu, Jianan; Liu, Lu; Peng, Kaiming; Yang, Gaoxiang; Liu, Jia

doi:10.1016/j.biortech.2016.01.073

Cited by 144 publications

(73 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results showed that yeast extract was a better nitrogen source for cell growth and lipid production. 29 Nevertheless, the lipid titer and lipid content were still maintained at high levels and the biomass yield was stable at high C/N ratios that ranged from 220 to 500, and this was possibly due to the production of citric acid at proper C/N ratios. The results are shown in Figure 2.…”

Section: Effect Of the C/n Ratio On Cell Growth And Lipid Accumulatmentioning

confidence: 98%

See 1 more Smart Citation

Enhanced lipid production by Rhodotorula glutinis CGMCC 2.703 using a two‐stage pH regulation strategy with acetate as the substrate

Zhou

Liu

et al. 2019

Energy Science & Engineering

View full text Add to dashboard Cite

In recent years, VFAs as a novel, inexpensive substrate for biodiesel production have become a hot research topic. The effect of pH on lipid production by Rhodotorula glutinis when using acetate as a substrate was studied. The optimized initial acetate concentration and C/N ratio were 23 g/L and 350, respectively. In 1‐L bioreactors containing an initial acetate concentration of 23 g/L, the most suitable pH for cell growth was 6.6, while pH 7.0 was found optimal for lipid production. In the pH‐stat fed‐batch fermentation, an increased biomass yield of 67.2 g/L was obtained at pH 6.6, while an increased lipid titer of 29.3 g/L was obtained at pH 7.0. When using a two‐stage pH regulation culture strategy, the biomass yield, lipid titer, and lipid content reached 68.2 g/L, 35.8 g/L, and 52.5%, respectively. Both the biomass yield and lipid production were obviously enhanced. Compared with the batch culture, the biomass yield, lipid titer, and lipid content were increased 15.6‐fold, 19.1‐fold, and 21.0%, respectively, by using the two‐stage pH regulation culture strategy. The composition of the main fatty acids was compared when acetate or glucose was used as the substrate. The composition of the lipids produced using both substrates was similar to that of palm oil, indicating that lipids produced by R. glutinis in the presence of acetate showed promise for use as a biodiesel feedstock.

show abstract

Section: Effect Of the C/n Ratio On Cell Growth And Lipid Accumulatmentioning

confidence: 98%

“…29 The acetate was consumed rapidly after 8 hours and was eventually completely consumed. The lag phase of R. glutinis was observed to be shorter than that of R. toruloides AS 2.1389 after a 3-day two-stage batch cultivation using an initial acetate concentration of 20 g/L.…”

Section: Batch Fermentation Of R Glutinis In a 5-l Bioreactor Usinmentioning

confidence: 99%

Enhanced lipid production by Rhodotorula glutinis CGMCC 2.703 using a two‐stage pH regulation strategy with acetate as the substrate

Zhou

Liu

et al. 2019

Energy Science & Engineering

View full text Add to dashboard Cite

show abstract

“…The lack of some nutrients such nitrogen, iron or magnesium can channel carbon flux promoting lipid accumulation as well as suppress lipid degradation pathways (Papanikolaou & Aggelis, ). Some researchers obtained an improvement in lipid content when increasing the C/N ratio (Huang et al, ). However, an excessively high C/N ratio leads would limit growth and lipid production (Béligon et al, ).…”

Section: Oleaginous Microorganisms For Oil Productionmentioning

confidence: 99%

Volatile fatty acids as novel building blocks for oil‐based chemistry via oleaginous yeast fermentation

Llamas

Magdalena

González‐Fernández

et al. 2019

Biotech & Bioengineering

View full text Add to dashboard Cite

Microbial oils are proposed as a suitable alternative to petroleum‐based chemistry in terms of environmental preservation. These oils have traditionally been studied using sugar‐based feedstock, which implies high costs, substrate limitation, and high contamination risks. In this sense, low‐cost carbon sources such as volatile fatty acids (VFAs) are envisaged as promising building blocks for lipid biosynthesis to produce oil‐based bioproducts. VFAs can be generated from a wide variety of organic wastes through anaerobic digestion and further converted into lipids by oleaginous yeasts (OYs) in a fermentation process. These microorganisms can accumulate in the form of lipid bodies, lipids of up to 60% wt/wt of their biomass. In this context, OY is a promising biotechnological tool for biofuel and bioproduct generation using low‐cost VFA media as substrates. This review covers recent advances in microbial oil production from VFAs. Production of VFAs via anaerobic digestion processes and the involved metabolic pathways are reviewed. The main challenges as well as recent approaches for lipid overproduction are also discussed.

show abstract

“…R. toruloides can metabolise the five- and six-carbon sugars liberated by degradation of lignocellulosic biomass, and can achieve a high lipid yield [7], [8]. R. toruloides can also metabolise other economically relevant feedstocks including acetic acid [9], glycerol [10] and inulin [11], [12]. Here we report the genome sequence of R. toruloides strain CGMCC 2.1609 which has inulinase activity [11].…”

Section: Introductionmentioning

confidence: 96%

Genome sequence of the oleaginous yeast Rhodotorula toruloides strain CGMCC 2.1609

et al. 2017

View full text Add to dashboard Cite

Most eukaryotic oleaginous species are yeasts and among them the basidiomycete red yeast, Rhodotorula (Rhodosporidium) toruloides (Pucciniomycotina) is known to produce high quantities of lipids when grown in nitrogen-limiting media, and has potential for biodiesel production. The genome of the CGMCC 2.1609 strain of this oleaginous red yeast was sequenced using a hybrid of Roche 454 and Illumina technology generating 13 × coverage. The de novo assembly was carried out using MIRA and scaffolded using MAQ and BAMBUS. The sequencing and assembly resulted in 365 scaffolds with total genome size of 33.4 Mb. The complete genome sequence of this strain was deposited in GenBank and the accession number is LKER00000000. The annotation is available on Figshare (doi:10.6084/m9.figshare.4754251).

show abstract

Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides

Cited by 144 publications

References 32 publications

Enhanced lipid production by Rhodotorula glutinis CGMCC 2.703 using a two‐stage pH regulation strategy with acetate as the substrate

Enhanced lipid production by Rhodotorula glutinis CGMCC 2.703 using a two‐stage pH regulation strategy with acetate as the substrate

Volatile fatty acids as novel building blocks for oil‐based chemistry via oleaginous yeast fermentation

Genome sequence of the oleaginous yeast Rhodotorula toruloides strain CGMCC 2.1609

Contact Info

Product

Resources

About