Culture Supernatant of Lactobacillus acidophilus Stimulates Proliferation of Embryonic Cells

Abstract

Our previous report showed that supernatants of Lactobacillus acidophilus (LS) cultures possessed chemotactic and angiogenic properties. Specifically, LS stimulated gene expression and the secretion of tumor necrosis factor-alpha (TNF-alpha), the proliferation of immune cells in vitro, and blood vessel formation. Chemotaxis and proliferation of inflammatory cells in vivo were also stimulated by LS. In the current study, we hypothesized that LS stimulates the growth and development of other rapidly dividing cells, including embryonic cells. The stimulatory effects of LS on a neuroblastoma cell line (Neuro-2a), chicken embryos, and bovine embryos were examined. The addition of LS to Neuro-2a cultures caused a proliferation of cells in a concentration-dependent manner. Pretreatment of LS at 56 degrees C for 30 mins did not affect its stimulatory activity. The administration of LS to the chorioallantoic membrane (CAM) of chicken-embryonated eggs for 1-2 days resulted in extensive thickening of the membrane. The thickening was due to the influx and proliferation of fibroblasts and inflammatory cells, the accumulation of loose connective tissue composed primarily of mucopolysaccharides, and/or the formation of blood vessels. Stimulatory effects of LS on bovine embryos were also observed. The treatment with LS significantly promoted the development of zygotes to the four-cell stage and from the four-cell stage to blastocysts. These results have confirmed our hypothesis that LS exerts a stimulatory effect on the cells of embryonic stages including neuroblastoma cells, the CAM of chicken embryos, and bovine embryos from zygotes to blastocysts.

Effects of concentrated supernatants recovered fromLactobacillus plantarumonEscherichia coligrowth and on the viability of a human promyelocytic cell line

“…acidophilus supernatants promote angiogenic and chemotactic activities, which stimulate the proliferation in vitro of macrophages, lymphocytes and embryonic cells (Halper et al. 2003; Li et al. 2005).…”

“…Recently, it has been demonstrated that Lact. acidophilus supernatants promote angiogenic and chemotactic activities, which stimulate the proliferation in vitro of macrophages, lymphocytes and embryonic cells (Halper et al 2003;Li et al 2005). In addition, soluble proteins synthesized by probiotic bacteria are able to regulate intestinal epithelial homeostasis, suggesting that natural components from these microorganisms may be applied for preventing cytokinemediated gastrointestinal pathologies (Yan et al 2007).…”

GanedenBC30™ cell wall and metabolites: anti-inflammatory and immune modulating effects in vitro

“…Conversely, gram positive bacteria engage the TLR-2 receptor via interaction with lipoteichoic acids present in the bacterial cell wall [23]. However, the mechanism of action for atypical cell wall components, as well as the metabolites secreted by these organisms needs further elucidation [24]. Bacillus coagulans is not a typical inhabitant of the human gastrointestinal tract.…”

Effect of Lactobacillus acidophilus supernatants on body weight and leptin expression in rats

“…Supernatants of Lactobacillus acidophilus were also proved to be effective against Helicobacter pylori in vitro and in vivo in people and were shown to possess antimicrobial activities against Bacillus anthracis and E. coli [ 3 ]. Our previous data have shown that Lactobacillus supernatants (LS) promote inflammatory response during tissue repair in rodents [ 5 ], stimulate proliferation of embryonic cells [ 6 ], and that subcutaneous injections of Lactobacillus supernatants into the ears of rats lead to angiogenesis [ 5 ]. Using a cytokine antibody array, leptin and several other cytokines (e.g., IL-6, IL-8 and TGFβ) were detected in medium conditioned by bovine endothelial cells exposed to LS (data not shown).…”