Culture supernatants from V. cholerae O1 ElTor strains isolated from different geographic areas induce cell vacuolation and cytotoxicity

Vidal, Jorge E.; Enríquez-Rincón, Fernando; Giono-Cerezo, Silvia; Ribas‐Aparicio, Rosa María; Figueroa-Arredondo, Paula

doi:10.1590/s0036-36342009000100009

Cited by 4 publications

(6 citation statements)

References 29 publications

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“…These results demonstrated the variety of titres that could be obtained in the evaluation of the biological vacuolating activity of different bacterial supernatants. These data are in agreement with previous reports, which showed that some pathogens produce toxins that affect host-cell stability and that could be present in bacterial supernatants ( Vidal et al 2009 ) so we propose the presence of a “vacuolating factor” in H. influenzae supernatants.…”

supporting

confidence: 93%

“…sobria ( Martins et al. 2007 ), ShlA of Serratia marcenscens , HlyA of Vibrio cholerae and VacA of Helicobacter pylori ( Figueroa-Arredondo et al 2001 , Vidal et al 2009 ). The aim of this study was to characterise the vacuolating effect induced by H. influenzae supernatants of ATCC strains, since this could be an undescribed pathogenic mechanism related with the vacuolating activity of certain autotransporter proteins, as a cell response against intracellular pathogens and also could be important for removing damaged organelles.…”

mentioning

confidence: 99%

“…Therefore, this showed that continuous exposure to the H. influenzae “vacuolating factor” can result in cell death. Vacuolation comprises extensive cell injury that has been described for many bacteria ( Vidal et al 2009 ). Therefore, host-cell vacuolation could be considered as a conserved pathogenic mechanism among certain medically important bacteria, which could improve their pathogenicity and establishment in host cells.…”

mentioning

confidence: 99%

“…Several authors have commented the relevance of identify at first, cytotoxic and vacuolating activity in culture supernatants of bacterial strains, in order to characterise it after ( Vidal et al 2009 ).…”

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confidence: 99%

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Cell vacuolation induced by Haemophilus influenzae supernatants in HEp-2 cells

Espinoza-Mellado

López-Villegas

Arteaga-Garibay

et al. 2013

Mem. Inst. Oswaldo Cruz

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Haemophilus influenzae belongs to respiratory tract microbiota. We observed vacuoles formation in previous studies with H. influenzae culture supernatants, so in this work we characterised that cytotoxic effect. We observed an abundant production of acidic cytoplasmic vacuoles due to the presence of a “vacuolating factor” in H. influenzae supernatants which was characterised as thermolabile. Greatest vacuolating activity was observed when utilizing the fraction > 50 kDa. The presence of a large number of vacuoles in HEp-2 cells was verified by transmission electron microscopy and some vacuoles were identified with a double membrane and/or being surrounded by ribosomes. These results suggest similar behaviour to that of vacuolating effects described by autotransporter proteins an undescribed cytotoxic effect induced by H. influenzae .

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confidence: 93%

mentioning

confidence: 99%

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confidence: 99%

mentioning

confidence: 99%

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Cell vacuolation induced by Haemophilus influenzae supernatants in HEp-2 cells

Espinoza-Mellado

López-Villegas

Arteaga-Garibay

et al. 2013

Mem. Inst. Oswaldo Cruz

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“…The drop in the ATP level seem to be due to the activation of ATP-dependent ion pumps similar to that observed in PLCD after opening of BKCa. Low VCC concentrations triggers autophagy; it is localized to autolysosomes and induces their vacuolization [143, 204–207] (Table 1). In contrast to VacA, VCC-induced vacuolization does not depend on the GTPase Rab7 and V-ATPase [205].…”

Section: Vacuolization and Unknown Cell Death Pathwaysmentioning

confidence: 99%

Cytoplasmic vacuolization in cell death and survival

et al. 2016

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Cytoplasmic vacuolization (also called cytoplasmic vacuolation) is a well-known morphological phenomenon observed in mammalian cells after exposure to bacterial or viral pathogens as well as to various natural and artificial low-molecular-weight compounds. Vacuolization often accompanies cell death; however, its role in cell death processes remains unclear. This can be attributed to studying vacuolization at the level of morphology for many years. At the same time, new data on the molecular mechanisms of the vacuole formation and structure have become available. In addition, numerous examples of the association between vacuolization and previously unknown cell death types have been reported. Here, we review these data to make a deeper insight into the role of cytoplasmic vacuolization in cell death and survival.

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Vibrio cholerae hemagglutinin(HA)/protease: An extracellular metalloprotease with multiple pathogenic activities

Benítez

Silva

2016

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Vibrio cholerae of serogroup O1 and O139, the etiological agent of the diarrheal disease cholera, expresses the extracellular Zn-dependent metalloprotease hemagglutinin (HA)/protease also reported as vibriolysin. This enzyme is also produced by non-O1/O139 (non-cholera) strains that cause mild, sporadic illness (i.e. gastroenteritis, wound or ear infections). Orthologs of HA/protease are present in other members of the Vibrionaceae family pathogenic to humans and fish. HA/protease belongs to the M4 neutral peptidase family and displays significant amino acid sequence homology to Pseudomonas aeruginosa elastase (LasB) and Bacillus thermoproteolyticus thermolysin. It exhibits a broad range of potentially pathogenic activities in cell culture and animal models. These activities range from the covalent modification of other toxins, the degradation of the protective mucus barrier and disruption of intestinal tight junctions. Here we review (i) the structure and regulation of HA/protease expression, (ii) its interaction with other toxins and the intestinal mucosa and (iii) discuss the possible role(s) of HA/protease in the pathogenesis of cholera.

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Culture supernatants from V. cholerae O1 ElTor strains isolated from different geographic areas induce cell vacuolation and cytotoxicity

Cited by 4 publications

References 29 publications

Cell vacuolation induced by Haemophilus influenzae supernatants in HEp-2 cells

Cell vacuolation induced by Haemophilus influenzae supernatants in HEp-2 cells

Cytoplasmic vacuolization in cell death and survival

Vibrio cholerae hemagglutinin(HA)/protease: An extracellular metalloprotease with multiple pathogenic activities

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