Cultured autologous keratinocytes on a cell-free dermis in the treatment of full-thickness wounds

Gustafson, Carl-Johan; Kratz, Gunnar

doi:10.1016/s0305-4179(99)00004-2

Cited by 36 publications

(17 citation statements)

References 12 publications

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“…Recently, according to the development of a cell culturing technique, full-thickness skin equivalents composed of cultured keratinocytes and dermal components in skin defects showed faster re-epithelialization and less wound contraction [27][28][29]. Ophof et al evaluated the efficacy of two types of tissue-engineered mucosal substitutes implanted in full-thickness wounds in the palatal mucosa of dogs [30].…”

Section: Discussionmentioning

confidence: 99%

Collagen-Gelatin Scaffold Impregnated with bFGF Accelerates Palatal Wound Healing of Palatal Mucosa in Dogs

Ayvazyan

Morimoto

Kanda

et al. 2011

Journal of Surgical Research

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Section: Discussionmentioning

confidence: 99%

Collagen-Gelatin Scaffold Impregnated with bFGF Accelerates Palatal Wound Healing of Palatal Mucosa in Dogs

Ayvazyan

Morimoto

Kanda

et al. 2011

Journal of Surgical Research

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“…By culturing and transplanting the cells in a suspension rather than as a sheet, the use of enzymes like Dispase ® can be avoided. Keratinocytes in suspension can then be transported from laboratory to patient in a handful of small vials ( Figure 1C) and be stored (frozen) at the clinic to be transplanted when the wound surfaces are ready [7]. The single cell suspension of keratinocytes can then be transplanted to the patient with whatever method is available such as being spray-painted on the wound surfaces with or without fibringlue ( Figure 1D) [8,9].…”

Section: Introductionmentioning

confidence: 99%

Transplantation of cultured human keratinocytes in single cell suspension: A comparative in vitro study of different application techniques

Fredriksson

Kratz

Huss

2008

Burns

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Transplantation of autologous cultured keratinocytes in single cell suspension is useful in the treatment of burns. The reduced time needed for culture, and the fact that keratinocytes in suspension can be transported from the laboratory to the patient in small vials, thus reducing the costs involved and be stored (frozen) in the clinic for transplantation when the wound surfaces are ready, makes it appealing. We found few published data in the literature about actual cell survival after transplantation of keratinocytes in single cell suspension and so did a comparative in vitro study, considering commonly used application techniques. Human primary keratinocytes were transplanted in vitro in a standard manner using different techniques. Keratinocytes were counted before and after transplantation, were subsequently allowed to proliferate, and counted again on days 4, 8, and 14 by vital staining. Cell survival varied, ranging from 47% to >90%, depending on the technique. However, the proliferation assays showed that the differences in numbers diminished after 8 days of culture. Our findings indicate that a great number of cells die during transplantation but that this effect is diminished if cells are allowed to proliferate in an optimal milieu. A burned patient's wounds cannot be regarded as the optimal milieu, and using less harsh methods of transplantation may increase the take rate and wound closing properties of autologous keratinocytes transplanted in a single cell suspension.

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“…5,6 To overcome these adverse events, artificial dermal substitutes have been used to assist in keratinocyte growth. [7][8][9][10][11][12] One dermal analog used for treatment of full-thickness wounds is an acellular human cadaver dermis, AlloDerm (LifeCell, Branchburg, NJ).…”

Section: Introduction Smentioning

confidence: 99%

Evaluation of Transplanted Tissue-Engineered Oral Mucosa Equivalents in Severe Combined Immunodeficient Mice

et al. 2003

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The aim of this study was to determine the optimal stage of development at which transplant human ex vivo-produced oral mucosa equivalents (EVPOMEs) in vivo. EVPOMEs were generated in a serum-free culture system, without the use of an irradiated xenogeneic feeder layer, by seeding human oral keratinocytes onto a human cadaveric dermal equivalent, AlloDerm. EVPOMEs were cultured for 4 days submerged and then for 7 or 14 days at an air-liquid interface to initiate stratification before transplantation into SCID mice. AlloDerm, without epithelium, was used as a control. Mice were killed on days 3, 10, and 21 posttransplantation. Epithelium of the transplanted EVPOMEs was evaluated with the differentiation marker keratin 10/13. Dermal microvessel ingrowth was determined by immunohistochemistry with a mouse vascular marker, lectin binding from Triticum vulgaris. The presence and stratification of the epithelium were correlated with revascularization of the underlying dermis. The microvessel density of AlloDerm without epithelium was less than that of EVPOMEs with an epithelial layer. Microvessel density of the dermis varied directly with the degree of epithelial stratification of the EVPOMEs. The EVPOMEs cultured at an air-liquid interface for 7 days had the optimal balance of neoangiogenesis and epithelial differentiation necessary for in vivo grafting. 163

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Cultured autologous keratinocytes on a cell-free dermis in the treatment of full-thickness wounds

Cited by 36 publications

References 12 publications

Collagen-Gelatin Scaffold Impregnated with bFGF Accelerates Palatal Wound Healing of Palatal Mucosa in Dogs

Collagen-Gelatin Scaffold Impregnated with bFGF Accelerates Palatal Wound Healing of Palatal Mucosa in Dogs

Transplantation of cultured human keratinocytes in single cell suspension: A comparative in vitro study of different application techniques

Evaluation of Transplanted Tissue-Engineered Oral Mucosa Equivalents in Severe Combined Immunodeficient Mice

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