Cultured cell lines from human breast cancer biopsies and xenografts

Lewko, Walter M.; Vaghmar, Rupa; Hubbard, Diane; Moore, MA; He, Yangang; Chang, Lee; Husseini, Salah; Wallwork, Karen; Thurman, Gary B.; Oldham, Robert K.

doi:10.1007/bf01806292

Cited by 10 publications

(8 citation statements)

References 13 publications

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“…The biopsy and xenograft cell lines were very similar in their properties. We have also observed close similarity in several other biopsy-xenograft cell lines from various types of tumors including breast [9] and colon [16] carcinomas.…”

Section: Discussionsupporting

confidence: 51%

“…Detailed methodology for tumor acquisition, processing, and propagation are described in another paper [9]. A 15 g portion of the tumor was flown to the laboratory in cold tissue culture medium.…”

Section: Methodsmentioning

confidence: 99%

“…They were plated at 1 million cells per T25 flask, in 5 ml of growth medium. Enzymatically dissociated cells were prepared using collagenase and DNase as described [9]. Excess red blood cells were reduced by lysis for 5 min at 37°C in a solution of 17 mM Trizma base (Sigma Chem.…”

Section: Methodsmentioning

confidence: 99%

“…We have received over 85 breast cancer specimens as part of a program in tumor processing for patient biotherapy [9]. One of these breast cancers was a malignant cystosarcoma phylloides.…”

Section: Introductionmentioning

confidence: 99%

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Cultured breast cystosarcoma phylloides cells and applications to patient therapy

Lewko¹,

Vaghmar²,

Maleckar³

et al. 1990

Breast Cancer Res Tr

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Malignant cystosarcoma phylloides (CP) is a relatively rare cancer of the breast. A CP tumor was processed as part of a tumor acquisition, propagation, and preservation program in patient biotherapy. Two tissue culture cell lines were developed from this tumor, one directly from the biopsy, another from a xenograft tumor grown in athymic mice. The two cell lines were similar in character. There was strong immunochemical reactivity with antibodies to vimentin, type I collagen, and type III collagen. There was no reactivity with antibodies to cytokeratin and epithelial membrane antigen. Both cell lines were aneuploid, clonogenic in soft agar, and tumorigenic in nude mice. 5 alpha-dihydrotestosterone and thyroxine added to the culture medium stimulated growth, while testosterone, 17 beta-estradiol, and 4-hydroxytamoxifen were without effect. Dexamethasone and cortisol were inhibitory at high doses (10(-6) M). Dibutyryl cyclic AMP, theophylline, and vitamin C were all inhibitory. The biopsy contained tumor-infiltrating lymphocytes which proliferated in cultures containing interleukin 2. The expanded lymphocytes were activated T cells which had the capacity to lyse tumor cells. These results suggest possibilities in the therapy of cystosarcoma phylloides involving vitamin C, certain hormones, and tumor-infiltrating lymphocytes.

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Section: Discussionsupporting

confidence: 51%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…We have received over 85 breast cancer specimens as part of a program in tumor processing for patient biotherapy [9]. One of these breast cancers was a malignant cystosarcoma phylloides.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cultured breast cystosarcoma phylloides cells and applications to patient therapy

Lewko¹,

Vaghmar²,

Maleckar³

et al. 1990

Breast Cancer Res Tr

Self Cite

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“…2) in the case of BRXCoSX. In preliminary studies, ECM prepared by ammonium hydroxide lysis15 of a squamous cell carcinoma of the lung did not permit attachment and growth, whereas the ECM of two No. 8 CULTURE O F COLORECTAL CA * Lewko et af.…”

Section: Discussionmentioning

confidence: 95%

Tumor acquisition, propagation, and preservation. The culture of human colorectal cancer

Lewko

Ladd

Hubbard

et al. 1989

Cancer

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Fourteen new colorectal cancer cell lines were developed as part of a tumor acquisition, propagation, and preservation program for biotherapy. Fifty-six specimens were received. Nine cell lines were generated from biopsies; seven of these cell lines were from metastatic lesions. Five additional cell lines were developed from xenografts grown in nude mice. Biopsies that produced three of these xenografts gave rise to parallel culture cell lines. Biopsy-derived and xenograft-derived cell lines from the same tumor behaved similarly in culture and exhibited similar markers when assessed immunohistochemically. Collagen substrate was beneficial in the primary culture of 50% of the specimens tested. Collagen was required for the successful propagation of two cell lines.

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Site‐specific experimental metastasis patterns of two human breast cancer cell lines in nude rats

Engebraaten

Fodstad

1999

Intl Journal of Cancer

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Animal models for breast cancer metastasis are valuable tools for studying mechanisms of metastasis and for pre‐clinical testing of anti‐metastatic therapy regimens. Using MA‐11 and MT‐1, two oestrogen and progesterone receptor–negative human breast cancer cell lines, we developed new models in nude rats with patterns of experimental metastasis resembling those frequently observed in humans. MA‐11 cells showed a clear preference for growth in the CNS. Fourteen of 15 animals injected with MA‐11 cells into the left ventricle of the heart developed hind‐leg paralysis, and tumours were observed in the spinal cord. MT‐1 cells consistently exhibited bone/bone marrow metastases after intracardial injection, in addition to tumours in the brain and spinal cord. When injected into the cisterna magna, both cell lines gave rise to leptomeningeal neoplastic disease. Injection of MA‐11 cells into the tibial bone marrow resulted in tumours in only 2 of 13 rats, whereas all animals injected with MT‐1 cells developed tumours. Only 2 of 6 rats injected i.v. with MA‐11 cells developed lung colonies compared with all 9 animals injected with MT‐1 cells. Cell‐surface expression of the following was examined: EGP2; MUC1; EGFr; E‐ and N‐cadherin; the α2, α3, α5, β1 and β4 integrins; c‐erb‐B2; and N‐CAM. c‐erb‐B2 was expressed in a higher percentage of the bone‐metastasizing MT‐1 cells than the MA‐11 cells, whereas E‐cadherin was expressed in MA‐11 but not MT‐1 cells. In animals injected with MA‐11 and MT‐1 cells in the left cardiac ventricle, treatment with cisplatin and doxorubicin did not improve survival. In summary, these clinically relevant animal models may be used for studies related to site‐specific growth and metastasis and for assessing effects of experimental therapy against human breast cancer. Int. J. Cancer82:219–225, 1999. © 1999 Wiley‐Liss, Inc.

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Cultured cell lines from human breast cancer biopsies and xenografts

Cited by 10 publications

References 13 publications

Cultured breast cystosarcoma phylloides cells and applications to patient therapy

Cultured breast cystosarcoma phylloides cells and applications to patient therapy

Tumor acquisition, propagation, and preservation. The culture of human colorectal cancer

Site‐specific experimental metastasis patterns of two human breast cancer cell lines in nude rats

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