Cultured chondrocyte and porcine cartilage-derived substance (PCS) construct as a possible dorsal augmentation material in rhinoplasty: A preliminary animal study

Kim, Yoo Suk; Park, Do Yang; Cho, Yong Hyun; Chang, Jae Won; Choi, Jae Won; Park, Joo Kyung; Min, Byung Hyun; Shin, Yoo Seob; Kim, Chul Ho

doi:10.1016/j.bjps.2014.12.017

Cited by 19 publications

(18 citation statements)

References 28 publications

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“…However, the degree of tip projection at 4 weeks was more prominent compared with those at 8 and 12 weeks. This discrepancy was likely due to the swollen status of the nasal tip 4 weeks after implantation . Moreover, there was no significant difference between 8 and 12 weeks, suggesting that the degree of tip projection was maintained throughout entire postoperative observation period.…”

Section: Discussionmentioning

confidence: 95%

“…There have been several recent studies in animal models of nasal reconstruction . However, among these studies, only two performed research combining animal models and tissue engineering, reporting on an in vivo study using biocompatible scaffolding materials such as PCL or extracellular matrix with chondrocytes . One study showed that that there was a favorable morphologic outcome with implants without postoperative complications over a 3‐month follow‐up period .…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, that study only inserted implants into the superficial space, which does not accurately reflect the effect of implant in deeper spaces (subperichondrial and intercartilaginous space). The other study used small button‐like PCL constructs implanted in the subcutaneous space of the nasal dorsum, which could not reflect implant changes related to mechanical loading from skin and soft tissue. Unlike previous studies, we classified and assessed the degree of nasal shape change objectively by photographic analysis, and our model intended to mimic true nasal surgery by inserting the implant into subperichondrial, intercartilaginous, and subcutaneous spaces.…”

Section: Discussionmentioning

confidence: 99%

“…9,[18][19][20][21] However, among these studies, only two performed research combining animal models and tissue engineering, 9,20 reporting on an in vivo study using biocompatible scaffolding materials such as PCL or extracellular matrix with chondrocytes. 9,20 One study showed that that there was a favorable morphologic outcome with implants without postoperative complications over a 3-month follow-up period. 9 However, the authors measured the degree of augmentation via visual assessment without objective analysis, impeding an accurate evaluation of the effect of implant on the nasal shape over time.…”

Section: Discussionmentioning

confidence: 99%

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New application of three‐dimensional printing biomaterial in nasal reconstruction

et al. 2017

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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New application of three‐dimensional printing biomaterial in nasal reconstruction

et al. 2017

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“…In previous studies, a variety of biomaterials exhibited good effects on the maintenance of cartilage [11][12][13][14][15]. However, whether a biomaterial can observably promote chondrogenic differentiation of human iPSCs in vitro has not reached a firm conclusion.…”

Section: Discussionmentioning

confidence: 99%

A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy

Chen

Gao

et al. 2020

Stem Cell Res Ther

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Background: Induced pluripotent stem cells (iPSCs) exhibit limitless pluripotent plasticity and proliferation capability to provide an abundant cell source for tissue regenerative medicine. Thus, inducing iPSCs toward a specific differentiation direction is an important scientific question. Traditionally, iPSCs have been induced to chondrocytes with the help of some small molecules within 21-36 days. To speed up the differentiation of iPSCs, we supposed to utilize bioactive ceramics to assist chondrogenic-induction process. Methods: In this study, we applied ionic products (3.125~12.5 mg/mL) of the lithium-containing bioceramic (Li 2 Ca 4 Si 4 O 13 , L2C4S4) and individual Li + (5.78~23.73 mg/L) in the direct chondrogenic differentiation of human iPSCs. Results: Compared to pure chondrogenic medium and extracts of tricalcium phosphate (TCP), the extracts of L2C4S4 at a certain concentration range (3.125~12.5 mg/mL) significantly enhanced chondrogenic proteins Type II Collagen (COL II)/Aggrecan/ SRY-Box 9 (SOX9) synthesis and reduced hypertrophic protein type X collagen (COL X)/matrix metallopeptidase 13 (MMP13) production in iPSCs-derived chondrocytes within 14 days, suggesting that these newly generated chondrocytes exhibited favorable chondrocytes characteristics and maintained a low-hypertrophy state. Further studies demonstrated that the individual Li + ions at the concentration range of 5.78~23.73 mg/L also accelerated the chondrogenic differentiation of iPSCs, indicating that Li + ions played a pivotal role in chondrogenic differentiation process.Conclusions: These findings indicated that lithium-containing bioceramic with bioactive specific ionic components may be used for a promising platform for inducing iPSCs toward chondrogenic differentiation and cartilage regeneration.

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Customizable, biocompatible implants for dorsal nasal augmentation: An in vivo pilot study of eight polylactic acid scaffold designs

O'Connell,

Vernice,

Matavosian

et al. 2024

J Biomedical Materials Res

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Augmentation of the nasal dorsum often requires implantation of structural material. Existing methods include autologous, cadaveric or alloplastic materials and injectable hydrogels. Each of these options is associated with considerable limitations. There is an ongoing need for precise and versatile implants that produce long‐lasting craniofacial augmentation. Four separate polylactic acid (PLA) dorsal nasal implant designs were 3D‐printed. Two implants had internal PLA rebar of differing porosities and two were designed as “shells” of differing porosities. Shell designs were implanted without infill or with either minced or zested processed decellularized ovine cartilage infill to serve as a “biologic rebar”, yielding eight total treatment groups. Scaffolds were implanted heterotopically on rat dorsa (N = 4 implants per rat) for explant after 3, 6, and 12 months followed by volumetric, histopathologic, and biomechanical analysis. Low porosity implants with either minced cartilage or PLA rebar infill had superior volume retention across all timepoints. Overall, histopathologic and immunohistochemical analysis showed a resolving inflammatory response with an M1/M2 ratio consistently favoring tissue regeneration over the study course. However, xenograft cartilage showed areas of degradation and pro‐inflammatory infiltrate contributing to volume and contour loss over time. Biomechanical analysis revealed all constructs had equilibrium and instantaneous moduli higher than human septal cartilage controls. Biocompatible, degradable polymer implants can induce healthy neotissue ingrowth resulting in guided soft tissue augmentation and offer a simple, customizable and clinically‐translatable alternative to existing craniofacial soft tissue augmentation materials. PLA‐only implants may be superior to combination PLA and xenograft implants due to contour irregularities associated with cartilage degradation.

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Cultured chondrocyte and porcine cartilage-derived substance (PCS) construct as a possible dorsal augmentation material in rhinoplasty: A preliminary animal study

Cited by 19 publications

References 28 publications

New application of three‐dimensional printing biomaterial in nasal reconstruction

New application of three‐dimensional printing biomaterial in nasal reconstruction

A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy

Customizable, biocompatible implants for dorsal nasal augmentation: An in vivo pilot study of eight polylactic acid scaffold designs

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