Cultured Human Corneal Endothelial Cell Transplantation with a Collagen Sheet in a Rabbit Model

Mimura, Tatsuya; Yamagami, Satoru; Yokoo, Seiichi; Usui, Tomohiro; Tanaka, Keisuke; Hattori, Shunji; Irie, Shinkichi; Miyata, Kazunori; Araie, Makoto; Amano, Shiro

doi:10.1167/iovs.03-1174

Cited by 252 publications

(198 citation statements)

References 26 publications

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“…Materials like collagen, amniotic membrane, and biodegradable polymers have successfully been used in culture media and animal models. [80][81][82] Porcine corneal matrix and Descemet's membrane have also been used as scaffolds for transplanted cells allowing good transparency in cat and rat corneas, and maintaining cell properties after the transplant. 60,83 Human decellularized stromas have been used to culture HCECs, allowing them to retain the expression of Na þ /K þ -ATPase and ZO-1 markers for greater than 14 days.…”

Section: Cell Therapymentioning

confidence: 99%

Corneal endothelium: developmental strategies for regeneration

Zavala

Jaime

Barrientos

et al. 2013

Eye

113

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The main treatment available for restoration of the corneal endothelium is keratoplasty. This procedure is faced with several difficulties, including the shortage of donor tissue, postsurgical complications associated with the use of drugs to prevent immune rejection, and a significant increase in the occurrence of glaucoma. Recently, surgical procedures such as Descemet's stripping endothelial keratoplasty have focused on the transplant of corneal endothelium, yielding better visual results but still facing the need for donor tissue. The emergent strategies in the field of cell biology and tissue cultivation of corneal endothelial cells aim at the production of transplantable endothelial cell sheets. Cell therapy focuses on the culture of corneal endothelial cells retrieved from the donor, in the donor's cornea, followed by transplantation into the recipient. Recently, research has focused on overcoming the challenge of harvesting human corneal endothelial cells and the generation of new biomembranes to be used as cell scaffolds in surgical procedures. The use of corneal endothelial precursors from the peripheral cornea has also demonstrated to be effective and represents a valuable tool for reducing the risk of rejection in allogeneic transplants. Several animal model reports also support the use of adult stem cells as therapy for corneal diseases. Current results represent important progresses in the development of new strategies based on alternative sources of tissue for the treatment of corneal endotheliopathies. Different databases were used to search literature: PubMed, Google Books, MD Consult, Google Scholar, Gene Cards, and NCBI Books. The main search terms used were: 'cornea AND embryology AND transcription factors', 'human endothelial keratoplasty AND risk factors', '(cornea OR corneal) AND (endothelium OR endothelial) AND cell culture', 'mesenchymal stem cells AND cell therapy', 'mesenchymal stem cells AND cornea', and 'stem cells AND (cornea OR corneal) AND (endothelial OR endothelium)'.

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Section: Cell Therapymentioning

confidence: 99%

Corneal endothelium: developmental strategies for regeneration

Zavala

Jaime

Barrientos

et al. 2013

Eye

113

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“…Together with our findings, it appears that the TM stem/ progenitor cells are present in both primate and non-primate animals. In addition, sphere culture provides us a useful tool to enrich the CE and TM progenitor cells, which may be used for transplantation as tried or suggested by others [75][76][77]. As a novel PET cell transplant approach might be applied in patients with CE diseases or glaucoma in the future, further research on these cells is required so that the finding of a replete non-primate source of PET cells is of enormous benefit.…”

mentioning

confidence: 99%

Bovine Posterior Limbus: An Evaluation of an Alternative Source for Corneal Endothelial and Trabecular Meshwork Stem/Progenitor Cells

Grierson

Sheridan

et al. 2015

Stem Cells and Development

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A growing body of evidence has revealed that stem-like cells in the posterior limbus of the eye between the corneal endothelium (CE) and trabecular meshwork (TM) may be able to rejuvenate these tissues in disease. However, these cells have not been clearly defined and we have named them PET cells (progenitor cells of the endothelium and trabeculum). A good and inexpensive animal model for PET cells is lacking, so we investigated bovine eyes as an effective large tissue source. We showed the presence of stem/progenitor cells in the bovine CE, transition zone, and TM in situ. Floating spheres cultured from the CE and TM showed similar stem cell marker expression patterns. Both the CE and TM spheres were bipotent and highly proliferative, but with limited secondary sphere-forming capability. They were highly prone to differentiate back into the cell type of their tissue of origin. It is speculated that the PET cells become more tissue-specific as they migrate away from their niche. Here, we showed that PET cells are present in the posterior limbus of bovine eyes and that they can be successfully cultured and expanded. PET cells represent an attractive target for developing new treatments to regenerate both the CE and TM, thereby reducing the requirement for donor tissue for corneal transplant and invasive treatments for glaucomatous patients.

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“…Mimura et al [44] used a network of loosely cross-linked type I collagen fibers for CECs. Corneal edema decreased rapidly after transplantation in DSAEK groups [44].…”

Section: Tissue Engineering Hcec Sheets Scaffoldsmentioning

confidence: 99%

“…Mimura et al [44] used a network of loosely cross-linked type I collagen fibers for CECs. Corneal edema decreased rapidly after transplantation in DSAEK groups [44]. Of the many scaffolds that are described in the literature and have proven its efficiency in vitro or in animal models, Koizumi et al [45] cultured monkey endothelial cell sheets on collagen type I carriers into monkey eyes; however, the corneas recovered their clarity only 6 months after transplantation.…”

Section: Tissue Engineering Hcec Sheets Scaffoldsmentioning

confidence: 99%

Concise Review: An Update on the Culture of Human Corneal Endothelial Cells for Transplantation

Parekh

Ferrari

Sheridan

et al. 2015

Stem Cells Translational Medicine

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The cornea forms the front window of the eye, enabling the transmission of light to the retina through a crystalline lens. Many disorders of the cornea lead to partial or total blindness, and therefore corneal transplantation becomes mandatory. Recently, selective corneal layer (as opposed to full thickness) transplantation has become popular because this leads to earlier rehabilitation and visual outcomes. Corneal endothelial disorders are a common cause of corneal disease and transplantation. Corneal endothelial transplantation is successful but limited worldwide because of lower donor corneal supply. Alternatives to corneal tissue for endothelial transplantation therefore require immediate attention. The field of human corneal endothelial culture for transplantation is rapidly emerging as a possible viable option. This manuscript provides an update regarding these developments. STEM CELLS TRANSLATIONAL MEDICINE 2016;5:258-264 SIGNIFICANCEThe cornea is the front clear window of the eye. It needs to be kept transparent for normal vision. It is formed of various layers of which the posterior layer (the endothelium) is responsible for the transparency of the cornea because it allows the transport of ions and solutes to and from the other layers of the cornea. Corneal blindness that results from the corneal endothelial dysfunction can be treated using healthy donor tissues. There is a huge demand for human donor corneas but limited supply, and therefore there is a need to identify alternatives that would reduce this demand. Research is underway to understand the isolation techniques for corneal endothelial cells, culturing these cells in the laboratory, and finding possible options to transplant these cells in the patients. This review article is an update on the recent developments in this field.

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Cultured Human Corneal Endothelial Cell Transplantation with a Collagen Sheet in a Rabbit Model

Cited by 252 publications

References 26 publications

Corneal endothelium: developmental strategies for regeneration

Corneal endothelium: developmental strategies for regeneration

Bovine Posterior Limbus: An Evaluation of an Alternative Source for Corneal Endothelial and Trabecular Meshwork Stem/Progenitor Cells

Concise Review: An Update on the Culture of Human Corneal Endothelial Cells for Transplantation

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