Cultured human decidual stromal cells express antigens associated with hematopoietic cells

Montes, María José Sánchez; Alemán, Pilar; García-Tortosa, Carmen; Borja, Concepción; Ruíz, Concepción; Olivares, Enrique G.

doi:10.1016/0165-0378(96)00954-0

Cited by 27 publications

(40 citation statements)

References 26 publications

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“…This finding is in accord with other reports of apoptosis of extravillous trophoblast [34][35][36]. It is likely that apoptosis of extravillous trophoblast is part of a controlled mechanism for trophoblast proliferation and invasion during normal pregnancy which is perturbed in molar pregnancy.…”

Section: Discussionsupporting

confidence: 76%

“…In addition, based on morphological criteria, at least some of the apoptotic cells were very likely to be typical decidualized stromal cells, although the lack of a specific marker for decidualized stromal cells limited our ability to demonstrate this unequivocally. Although they express CD10 in vitro [36,37], expression of CD10 by decidual stromal cells in situ is much less consistent, with many cells being negative or focally positive, unlike non-decidualized endometrial stromal cells which are consistently CD10 + [38,39]. Future studies using insulin-like growth factor binding protein (IGFBP-1), another marker of decidualization [40], may be possible but it should be noted that the IGFBP-1 status of decidua in molar pregnancy is unknown.…”

Section: Discussionmentioning

confidence: 99%

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No evidence for apoptosis of decidual leucocytes in normal and molar pregnancy: implications for immune privilege

Pongcharoen

Bulmer²,

Searle

2004

Clinical and Experimental Immunology

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SUMMARYComplete hydatidiform moles are totally paternally derived and represent complete allografts that might be expected to provoke maternal immune rejection. Our previous and other studies have shown expression of Fas by increased numbers of activated decidual CD4 + T cells in both complete and partial molar pregnancy as well as increased FasL + expression by molar trophoblasts compared with trophoblasts in normal pregnancies. As the Fas/FasL system represents a major apoptotic pathway that can play a role in immune privilege, the aim of this study was to investigate whether apoptosis of decidual immune cells, particularly T cells, could be responsible for maternal immune tolerance in molar pregnancy. Using terminal deoxynucleotidyl transferase (TdT)-mediated nick end-labelling (TUNEL), a significant increase in TUNEL + cells was demonstrated in decidua associated with partial ( P = 0·0052) and complete ( P = 0·0096) hydatidiform mole compared with normal early pregnancy. Co-labelling immunoperoxidase studies showed that the TUNEL + cells in both normal and molar pregnancies were not activated CD45RO + immune cells, CD3 + T cells, CD56 + uterine natural killer (NK) cells or CD14 + CD68 + macrophages. Double immunohistochemical labelling with antiactive caspase-3 and leucocyte markers confirmed the lack of leucocyte apoptosis. Double immunostaining with anticytokeratin to detect trophoblast and M30 CytoDeath, which detects a neoepitope of cytokeratin 18 revealed after caspase-mediated cleavage, revealed apoptotic extravillous trophoblast cells within decidual tissue. We conclude that there is no evidence that apoptosis of decidual leucocytes plays a role in maintaining maternal tolerance in either normal or molar pregnancy.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

No evidence for apoptosis of decidual leucocytes in normal and molar pregnancy: implications for immune privilege

Pongcharoen

Bulmer²,

Searle

2004

Clinical and Experimental Immunology

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“…To obtain a suspension of fresh cells containing FDC, we used a protocol similar to that reported by Montes et al (18) for fresh decidual stromal cells (DSC). The tonsil was washed in PBS and minced between two scalpels in a small volume of RPMI 1640 with 10% FCS.…”

Section: Isolation Of Fresh Fdcmentioning

confidence: 99%

Follicular Dendritic Cells Are Related to Bone Marrow Stromal Cell Progenitors and to Myofibroblasts

Muñoz-Fernández

Blanco

Frecha

et al. 2006

The Journal of Immunology

117

118

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Follicular dendritic cells (FDC) are involved in the presentation of native Ags to B cells during the secondary immune response. Some authors consider FDC to be hemopoietic cells, whereas others believe them to be mesenchymal cells. The low proportion of FDC in the lymphoid follicle, together with technical difficulties in their isolation, make these cells difficult to study. We show that Fibroblast Medium can be used successfully to isolate and maintain FDC lines. In this culture medium, we obtained 18 FDC lines from human tonsils, which proliferated for as long as 18 wk and showed a stable Ag phenotype as detected by flow cytometry and RT-PCR. FDC lines were CD45-negative and expressed Ags associated to FDC (CD21, CD23, CD35, CD40, CD73, BAFF, ICAM-1, and VCAM-1) and Ags specific for FDC (DRC-1, CNA.42, and HJ2). These cell lines were also able to bind B cells and secrete CXCL13, functional activities characteristic of FDC. Nevertheless, the additional expression of STRO-1, together with CD10, CD13, CD29, CD34, CD63, CD73, CD90, ICAM-1, VCAM-1, HLA-DR, alkaline phosphatase, and α-smooth muscle actin (α-SM actin) indicated that FDC are closely related to bone marrow stromal cell progenitors. The expression of α-SM actin also relates FDC with myofibroblasts. Like myofibroblasts, FDC lines expressed stress fibers containing α-SM actin and were able to contract collagen gels under the effect of TGFβ1 and platelet-derived growth factor. These findings suggest that FDC are a specialized form of myofibroblast and derive from bone marrow stromal cell progenitors.

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“…These cells have been shown to express antigens associated with hematopoietic cells (Montes et al 1996), although it was suggested that they are more closely related to bone marrow stromal precursors (García-Pacheco et al 2001). Their morphology and phenotype was described to be similar to myofibroblasts (Oliver et al 1999;Kimatrai et al 2003), α-smooth muscle actin (α-SMA) expressing fibroblastic cells with contractile activity that are involved in wound retraction.…”

Section: Introductionmentioning

confidence: 99%

Multipotent properties of myofibroblast cells derived from human placenta

et al. 2008

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Human uterine fibroblasts (HuF) isolated from the maternal part (decidua parietalis) of a term placenta provide a useful model of in vitro cell differentiation into decidual cells (decidualization), critical for successful pregnancy. After isolation, the cells adhere to plastic and have either a small round or spindle-shaped morphology which later changes into a flattened pattern in culture. HuF robustly proliferate in culture until passage 20 and form colonies when plated at low densities. The cells express the mesenchymal cell markers fibronectin, integrin-β1, ICAM-1 (CD54), and collagen I. Flow cytometry of HuF detected the presence of CD34, a marker of hematopoietic stem cell lineage, and an absence of CD10, CD11b/Mac, CD14, CD45, and HLA type II. Furthermore, they also express the pluripotency markers SSEA-1, SSEA-4, Oct-4, Stro-1, and TRA-1−81 as detected by confocal microscopy. Treatment for 14−21 days with differentiation-inducing media led to the differentiation of HuF into osteoblasts, adipocytes, and chondrocytes. The presence of α-smooth muscle actin, calponin, and myosin light chain kinase in cultured HuF implies their similarity to myofibroblasts. Treatment of the HuF with DMSO causes reversion back into the spindle-shaped morphology and a loss of myofibroblast characteristics, suggesting a switch into a less differentiated phenotype. The unique abilities of HuF to exhibit multipotency, even with myofibroblast characteristics, and their ready availability and low maintenance requirements make them an interesting cell model for further exploration as a possible tool for regenerative medicine.

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Cultured human decidual stromal cells express antigens associated with hematopoietic cells

Cited by 27 publications

References 26 publications

No evidence for apoptosis of decidual leucocytes in normal and molar pregnancy: implications for immune privilege

No evidence for apoptosis of decidual leucocytes in normal and molar pregnancy: implications for immune privilege

Follicular Dendritic Cells Are Related to Bone Marrow Stromal Cell Progenitors and to Myofibroblasts

Multipotent properties of myofibroblast cells derived from human placenta

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