Culturing duckweed in the field for starch accumulation

Xiao, Yao; Fang, Yang; Jin, Yanling; Zhang, Guohua; Zhao, Hai

doi:10.1016/j.indcrop.2013.04.017

Cited by 85 publications

(85 citation statements)

References 28 publications

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“…By contrast, previous studies have indicated that duckweed showed huge potential in high-starch accumulation during nutrient starvation (Tao et al, 2013;Xiao et al, 2013). The results of the high-starch accumulation experiment showed that the duckweed dry biomass per square meter increased from 16.31 ± 1.43 g/m 2 to 59.68 ± 0.81 g/m 2 in 11 days, and the average growth rate for 11 days was 3.93 ± 0.90 g DW/m 2 /d, which was lower than that in the wastewater.…”

Section: Accumulation Of High-starch In Duckweedmentioning

confidence: 81%

“…The nitrogen (N), crude protein, phosphorus and starch content were measured according to the method of Xiao et al (2013). The amino acid content was measured according to the national standard method (GB/T 5009. .…”

Section: Methodsmentioning

confidence: 99%

“…The NH 4 + AN, NO 3 À AN, NO 2 À AN and TP in wastewater were measured according to Xiao et al (2013) SPSS (Version 15.0, SPSS Inc., US) was used for data analysis, and analysis of variance (ANOVA) with a 95% confidence level was applied to examine the significant differences of all of the indicators in the variable treatments.…”

Section: Methodsmentioning

confidence: 99%

“…The mat density of the residual fresh water hyacinth after harvesting was maintained at 10-20 kg FW/m 2 . The fresh water hyacinth (approximately 400 g) and duckweed samples (approximately 200 g) were treated according to the method of Xiao et al (2013).…”

Section: Operation and Samplingmentioning

confidence: 99%

See 3 more Smart Citations

Potential of duckweed in the conversion of wastewater nutrients to valuable biomass: A pilot-scale comparison with water hyacinth

Zhao

Fang

Jin

et al. 2014

Bioresource Technology

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107

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Section: Accumulation Of High-starch In Duckweedmentioning

confidence: 81%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Operation and Samplingmentioning

confidence: 99%

See 2 more Smart Citations

Potential of duckweed in the conversion of wastewater nutrients to valuable biomass: A pilot-scale comparison with water hyacinth

Zhao

Fang

Jin

et al. 2014

Bioresource Technology

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107

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“…19,20 The crude protein content was measured as CP = K j N × 6.25, Xiao Yao. 21,22 Cellulose content was measured with spectrophotometry: 10 g of duckweed was placed in 1 L of water, 60 mL of 60% H 2 SO 4 were added, and the plants were left to decompose for 30 min. We added 2% anthrone reagent (v/v) to the hydrolyzed mixture, left it for 2 min, and then measured the absorbance under 620 nm.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Use of Duckweed (Landoltia punctata) as a Fermentation Substrate for the Production of Higher Alcohols as Biofuels

Zhao

Juan

et al. 2014

Energy Fuels

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Duckweed (Lemnaceae) is a family of aquatic plants with potential for use as the next generation of alternative energy feedstocks, yet little related information about producing higher alcohols from duckweed has been published. We investigated the process of producing higher alcohols from duckweed via fermentation. Results showed that these plants have a promising future as the basis for developing biofuels. This could be achieved through fermentation by yeasts, producing not only traditional forms of energy such as ethanol but also higher alcohols with high energy yields through bioconversion by Clostridium acetobutylicum, mutant yeast strains, and bioengineered strains of Escherichia coli. The concentrations of butanol and total solvent produced via fermentation by C. acetobutylicum CICC 8012 were 12.03 and 20.03 g/L using acid hydrolysate of duckweed versus 12.33 and 20.05 g/L using enzymatic hydrolysate. The yields obtained of 24.06 g/L ethanol and 680.36 mg/L of isopentanol from duckweed using acid hydrolysate are 15 times higher than what could be obtained through the fermentation of the mutation of yeast. In addition, we were able to obtain yields of 16.27 mg/L butanol, 24.68 mg/L isopentanol, and 195.85 mg/L pentanol from the acid hydrolysate of duckweed via fermentation by the bioengineered strains of E. coli. Our results illustrated that duckweed represent an ideal fermentation substrate: they require only simple pretreatment, without the need for supplementary nitrogen or strengthening with redox agents. This provides a foundation for further development of industrialized biofuel production using duckweed. ■ INTRODUCTIONGiven the widely recognized unsustainability of depending solely on petroleum for development and environmental problems, increasing efforts have been directed at synthesizing biofuels from renewable resources. 1−3 To date, several such renewable sources of energy have been developed, including ethanol from sugar cane, corn, and cassava and biodiesel from various plant oils such as coconut, palm, and rapeseed, which are also the sources of food for people and livestock. The increasing demand for ethanol and other biofuels could contribute to a global food shortage. Given this concern about the competing uses of resources for "food versus fuel", the rising scarcity and price of fossil fuels, and the need to protect global environmental resources and food security, there is an even greater need to find new, renewable, nonfood sources for biofuel production.The use of agricultural waste or byproducts and nonfood crop sources as cheap fermentation substrates to produce biofuels has high economic appeal. 4 Among nonfood crops, duckweed, a family of fast-growing, floating species that represent the world's smallest and simplest flowering plants, has been proposed as an inexpensive, sustainable source of plant biomass for producing biofuels. 5 There are many advantages to using duckweed in this regard, compared to other starch feedstocks such as corn and cassava. 6 First, duckweed h...

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Nutrient starvation and light deprivation effects on starch accumulation in Landoltia punctata cultivated on anaerobically digested dairy manure

Kruger

Chen

2020

J of Env Quality

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Duckweed has been recognized for its potential of producing biomass on nutrients from waste streams. Our research has shown that strains of duckweed can be successfully cultivated on anaerobically digested (AD) dairy manure under controlled levels of nitrogen (N) and phosphorus (P). The objective of this study was to explore the maximization of starch accumulation in Landoltia punctata (Mey.) Les & Crawford strain 0128 under different cultivation conditions using AD dairy manure as the nutrient source. Experimental results have shown that the most influential factors for starch accumulation in L. punctata were the nutrient concentration and the appropriate scheduling of nutrient starvation at the right growth stages. In our study, nutrient starvation for starch accumulation in L. punctata was achieved by incorporating nutrient addition of appropriately diluted AD dairy manure sequentially in a controlled manner under a constant light intensity of 3,000 lx. It was observed that a starch concentration of 30% (w/w) within the L. punctata biomass was achieved with an initial total N of 57.1 mg L−1 and a total P of 6.7 mg L−1 after a 30‐d cultivation. Under the abovementioned cultivation conditions, the duckweed L. punctata recovered 16.3% (±4.0%) of total N and 25.9% (±6.6%) of total P from AD manure into its biomass. It is concluded that L. punctata can be successfully cultivated on nutrients from dairy manure for starch production, which would achieve well‐being for dairy farmers by producing a feedstock for biofuels while treating dairy wastewater in an environmentally friendly manner.

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Culturing duckweed in the field for starch accumulation

Cited by 85 publications

References 28 publications

Potential of duckweed in the conversion of wastewater nutrients to valuable biomass: A pilot-scale comparison with water hyacinth

Potential of duckweed in the conversion of wastewater nutrients to valuable biomass: A pilot-scale comparison with water hyacinth

Use of Duckweed (Landoltia punctata) as a Fermentation Substrate for the Production of Higher Alcohols as Biofuels

Nutrient starvation and light deprivation effects on starch accumulation in Landoltia punctata cultivated on anaerobically digested dairy manure

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