Culturing functional cartilage tissue under a novel bionic mechanical condition

Sun, Ming; Lv, Dan; Zhang, Chunqiu; Zhu, Lei

doi:10.1016/j.mehy.2010.08.011

Cited by 13 publications

(7 citation statements)

References 16 publications

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“…Furthermore, hydrostatic pressure has been used to stimulate chondrogenic differentiation of bone marrow-derived (Luo and Seedhom 2007; Miyanishi et al 2006a, b; Wagner et al 2008), adipose-derived (Ogawa et al 2009), and synovium-derived stem cells (Sakao et al 2008) with promising results. Other loading regimes that have been investigated are shear loading, sliding/rolling indentation loading, tensile loading, centrifugal force, and gravity (Darling and Athanasiou 2003a, b; Schulz and Bader 2007; Khoshgoftar et al 2011; Sun et al 2010; Wimmer et al 2009) with mixed results. In conclusion, it is necessary to investigate which specific (combinations of) mechanical stimuli, as well as their parameters, result in optimal response of the cells in cultured constructs.…”

Section: Important Parameters For Cartilage Tissue Engineering Studiementioning

confidence: 99%

Tissue engineering of functional articular cartilage: the current status

2011

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Osteoarthritis is a degenerative joint disease characterized by pain and disability. It involves all ages and 70% of people aged >65 have some degree of osteoarthritis. Natural cartilage repair is limited because chondrocyte density and metabolism are low and cartilage has no blood supply. The results of joint-preserving treatment protocols such as debridement, mosaicplasty, perichondrium transplantation and autologous chondrocyte implantation vary largely and the average long-term result is unsatisfactory. One reason for limited clinical success is that most treatments require new cartilage to be formed at the site of a defect. However, the mechanical conditions at such sites are unfavorable for repair of the original damaged cartilage. Therefore, it is unlikely that healthy cartilage would form at these locations. The most promising method to circumvent this problem is to engineer mechanically stable cartilage ex vivo and to implant that into the damaged tissue area. This review outlines the issues related to the composition and functionality of tissue-engineered cartilage. In particular, the focus will be on the parameters cell source, signaling molecules, scaffolds and mechanical stimulation. In addition, the current status of tissue engineering of cartilage will be discussed, with the focus on extracellular matrix content, structure and its functionality.

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Section: Important Parameters For Cartilage Tissue Engineering Studiementioning

confidence: 99%

Tissue engineering of functional articular cartilage: the current status

2011

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“…The PCB underwent systematic validation revealing safe and reliable functionality to ensure defined dynamic mechanical loading of viable engineered tissues. As compared to previously described systems (Rath et al, 2008 ; Ballyns and Bonassar, 2010 ; Sittichokechaiwut et al, 2010 ; Sun et al, 2010 ; Lujan et al, 2011 ; Matziolis et al, 2011 ; Omata et al, 2012 ; Petri et al, 2012 ; Puetzer et al, 2012 ; Shahin and Doran, 2012 ), the PCB exhibits a broader operational range. It allows for physiological mechanical compression in a consistent and reliable manner in a range from approximately 10 N up to 1 kN, while maintaining compact dimensions.…”

Section: Discussionmentioning

confidence: 69%

“…As an alternative, in vitro model systems facilitate a methodical approach to study the impact of the mechanical stimuli during distinct phases of secondary fracture healing in a controlled manner. However, in vitro models have previously been limited to applying mechanical loads on cartilaginous tissues in order to develop more functional tissues or to study the impact of different loading regimes on chondrogenesis (Démarteau et al, 2003 ; Ballyns and Bonassar, 2010 ; Sun et al, 2010 ; Puetzer et al, 2012 ), but the effect of mechanical loading during the process of hypertrophic cartilage formation and remodeling, critical in fracture healing, has not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing

Hoffmann

Feliciano

Martín

et al. 2015

Front. Bioeng. Biotechnol.

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Secondary bone fracture healing is a physiological process that leads to functional tissue regeneration via endochondral bone formation. In vivo studies have demonstrated that early mobilization and the application of mechanical loads enhances the process of fracture healing. However, the influence of specific mechanical stimuli and particular effects during specific phases of fracture healing remain to be elucidated. In this work, we have developed and provided proof-of-concept of an in vitro human organotypic model of physiological loading of a cartilage callus, based on a novel perfused compression bioreactor (PCB) system. We then used the fracture callus model to investigate the regulatory role of dynamic mechanical loading. Our findings provide a proof-of-principle that dynamic mechanical loading applied by the PCB can enhance the maturation process of mesenchymal stromal cells toward late hypertrophic chondrocytes and the mineralization of the deposited extracellular matrix. The PCB provides a promising tool to study fracture healing and for the in vitro assessment of alternative fracture treatments based on engineered tissue grafts or pharmaceutical compounds, allowing for the reduction of animal experiments.

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“…In addition to mimic cartilage ECM environment, joint movements mechanical forces also played a crucial part in growth and development of articular cartilage tissue. It was found that stem cell performed better properties when cells grew in scaffold under external mechanical stimulations [23,35,36]. More recently, magnetic nanoparticles captured more attention to bioreactor as they were capable of providing different mechanical forces and were more suitable for applying loading to improve cell condensation and scaffold seeding efficiency [37][38][39].…”

Section: Gene Expression Of Cartilage Tissue-mimetic Pellets Under Phmentioning

confidence: 99%

Cartilage Tissue-Mimetic Pellets with Multifunctional Magnetic Hyaluronic Acid-Graft-Amphiphilic Gelatin Microcapsules for Chondrogenic Stimulation

et al. 2020

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Articular cartilage defect is a common disorder caused by sustained mechanical stress. Owing to its nature of avascular, cartilage had less reconstruction ability so there is always a need for other repair strategies. In this study, we proposed tissue-mimetic pellets composed of chondrocytes and hyaluronic acid-graft-amphiphilic gelatin microcapsules (HA-AGMCs) to serve as biomimetic chondrocyte extracellular matrix (ECM) environments. The multifunctional HA-AGMC with specific targeting on CD44 receptors provides excellent structural stability and demonstrates high cell viability even in the center of pellets after 14 days culture. Furthermore, with superparamagnetic iron oxide nanoparticles (SPIOs) in the microcapsule shell of HA-AGMCs, it not only showed sound cell guiding ability but also induced two physical stimulations of static magnetic field(S) and magnet-derived shear stress (MF) on chondrogenic regeneration. Cartilage tissue-specific gene expressions of Col II and SOX9 were upregulated in the present of HA-AGMC in the early stage, and HA-AGMC+MF+S held the highest chondrogenic commitments throughout the study. Additionally, cartilage tissue-mimetic pellets with magnetic stimulation can stimulate chondrogenesis and sGAG synthesis.

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Culturing functional cartilage tissue under a novel bionic mechanical condition

Cited by 13 publications

References 16 publications

Tissue engineering of functional articular cartilage: the current status

Tissue engineering of functional articular cartilage: the current status

Novel perfused compression bioreactor system as an in vitro model to investigate fracture healing

Cartilage Tissue-Mimetic Pellets with Multifunctional Magnetic Hyaluronic Acid-Graft-Amphiphilic Gelatin Microcapsules for Chondrogenic Stimulation

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