Culturing, Identification and Drug Resistance of Mycobacterium Tuberculosis in Sputum Specimen

Abstract

The development of low-cost, fast, and reliable methods for detecting Mycobacterium tuberculosis (MTB) infection and drug susceptibility is critical for tuberculosis control. The new microscopic examination of liquid drug susceptibility assay (MODS) examines early MTB colonies in a liquid medium, which permits for more convenient diagnosis and testing of drug susceptibility. The sensitivity of MODS (91%) was superior as compared to the sensitivity of different culture methods (92%). The MGIT and MODS were used to monitor tuberculosis-positive sputum samples for isoniazid and rifampin susceptibility. Concordance between MODS and MGIT was found in 89 percent of cases. MODS are the fastest method used for diagnostic and susceptibility testing (median, 10.0 or 9 days). MODS is a fast, low-cost, responsive, and particular method for detecting and testing MTB susceptibility; it is specifically well-suited utilize in growing countries with high infection rates and a growing number of multidrug-resistant cases.

Molecular Approaches for Detection of Rifampin-Resistant Mycobacterium tuberculosis and Its Associated Risk Factors among General Population

“…Worldwide It is the second primary death cause after Acquired Immunodeficiency Syndrome (AIDS). It is a global health problem common in developing countries and its early detection and proper treatment are important to save human life [1][2][3]. Although GeneXpert Mycobacterium tuberculosis/Rifampicin (MTB/RIF) assay is a widely used diagnostic procedure for tuberculosis but still incidence of tuberculosis is 4.1 million people worldwide.…”