A total of 125-day-old Arbor Acre broiler chickens fed broiler starter till day 20 were used to investigate the effect of low calcium and phosphorus diets supplemented with phytase and cholecalciferol. On day 21, the chicks were weighed and allotted to 5 treatments with 5 replicates of 5 birds each in a randomized complete block design. Between days 25 and 27 post-hatch, samples of fresh excreta were collected once daily from each cage. On day 28, the birds were euthanized by carbon (IV) oxide asphyxiation and dissected to obtain and digesta from the 2/3rd of the ileum. All data were analysed using the GLM procedure in SAS. The treatments comprised a positive control (PC, T1) without cottonseed meal (CSM) and four CSM-based semi-purified diets; negative control (NC, T2), NC + vitamin D3-10,000 IU/Kg (T3), NC + phytase (T4) and NC + phytase + vitamin D3 (T5). Titanium dioxide (5 g/Kg) was included as marker. Digestible P was significantly (P < 0.05) improved for birds on T5 when compared with the other four diets.Digestible P in T4 did not differ (P > 0.05) from the birds on T1. Ileal digestible phosphorus (IDP) significantly (P < 0.05) reduced with a corresponding increase in the percentage apparent phosphorus digestibility (APD) in T3, while T4 and T5 did not differ from T1 in the two parameters. It can be concluded that low calcium and phosphorus diets supplemented with phytase and vitamin D3 on cottonseed meal improved digestible P in broiler chickens.