CuO nanorod-decorated hemin-graphene with enhanced peroxidase-mimicking performance for the colorimetric and electrochemical determination of 4-aminophenol with a smartphone

Li, Miaomiao; Peng, Xiuying; Liu, Zhiguang; Dai, Yu; Fan, Lifang; Guo, Yujing

doi:10.1039/d3an00327b

Cited by 4 publications

(1 citation statement)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, this result suggests that TMB can be used as an effective redox mediator for improving the sensitivity and lowering the detection limit. Therefore, TMB is known as a detection medium in various electrochemical biosensors [34]. Taking advantage of this property, electrochemical sensors using H 2 O 2 and TMB as reaction substrates were constructed.…”

Section: Optimization Of the Components In The Reaction Systemmentioning

confidence: 99%

A Multienzyme Reaction-Mediated Electrochemical Biosensor for Sensitive Detection of Organophosphorus Pesticides

Ji,

Tang,

Wen

et al. 2024

Biosensors

View full text Add to dashboard Cite

Ethephon (ETH), a commonly employed growth regulator, poses potential health risks due to its residue in fruits and vegetables, leading to both acute and subchronic toxicity. However, the detection accuracy of ETH is compromised by the color effects of the samples during the detection process. In this work, a multienzyme reaction-mediated electrochemical biosensor (MRMEC) was developed for the sensitive, rapid, and color-interference-resistant determination of ETH. Nanozymes Fe3O4@Au–Pt and graphene nanocomplexes (GN–Au NPs) were prepared as catalysts and signal amplifiers for MRMEC. Acetylcholinesterase (AChE), acetylcholine (ACh), and choline oxidase (CHOx) form a cascade enzyme reaction to produce H2O2 in an electrolytic cell. Fe3O4@Au–Pt has excellent peroxidase-like activity and can catalyze the oxidation of 3,3′,5,5′-tetramethvlbenzidine (TMB) in the presence of H2O2, resulting in a decrease in the characteristic peak current of TMB. Based on the inhibitory effect of ETH on AChE, the differential pulse voltammetry (DPV) current signal of TMB was used to detect ETH, offering the limit of detection (LOD) of 2.01 nmol L−1. The MRMEC method effectively analyzed ETH levels in mangoes, showing satisfactory precision (coefficient of variations, 2.88–15.97%) and recovery rate (92.18–110.72%). This biosensor holds promise for detecting various organophosphorus pesticides in food samples.

show abstract

Section: Optimization Of the Components In The Reaction Systemmentioning

confidence: 99%