Cupric oxide nanoparticles‐enhanced chemiluminescence method for measurement of β‐lactam antibiotics

Abstract

A simple, sensitive cupric oxide nanoparticles (CuO NPs) enhanced chemiluminescence (CL) method was developed for the measurement of β-lactam antibiotics, including amoxicillin and cefazolin sodium. The method was based on suppression of the CuO NPs-luminol-H2O2 CL reaction by β-lactam antibiotics. Experimental parameters that influenced the inhibitory effect of the antibiotic drugs on the CL system, such as NaOH (mol/L), luminol (µmol/L), H2O2 (mol/L) and CuO NPs (mg/L) concentrations, were optimized. Calibration graphs were linear and had dynamic ranges of 1.0 × 10(-6) to 8.0 × 10(-6) mol/L and 3.0 × 10(-5) to 5.0 × 10(-3) mol/L for amoxicillin and cefazolin sodium, respectively, with corresponding detection limits of 7.9 × 10(-7) mol/L and 1.8 × 10(-5) mol/L. The relative standard deviations of five replicate measurements of 5.0 × 10(-6) amoxicillin and 5 × 10(-4) cefazolin sodium were 5.43 and 5.01%, respectively. The synthesized CuO NPs were characterized by X-ray diffraction (XRD) and transmission electronmicroscopy (TEM). The developed approach was exploited successfully to measure antibiotics in pharmaceutical preparations.

Enhanced luminol–O2 chemiluminescence reaction by CuO nanoparticles as oxidase mimics and its application for determination of ceftazidime

“…25,26 The application realm of the system was then further expanded by other researchers. [27][28][29][30][31][32] However, to the best of our knowledge, the oxidase-like activity of CuO NPs, a discovered 33 but less known property of CuO NPs, has somehow been neglected and has not yet been incorporated in analytical applications. It should be noted that the oxidase-like activity of V 2 O 5 nanowires 34 and CoFe 2 O 4 NPs 35 toward the luminol-O 2 CL reaction has also been studied.…”

Synthesis of a novel diaquabis(1,10-phenanthroline)copper(II)chloride complex and its voltammetric application for detection of amoxicillin in pharmaceutical and biological samples

“…Many analytical techniques for the determination of AMX have been described, including spectrophotometry [ 12 ], chromatography [ 13 ] and chemiluminescence [ 14 ]. However, these techniques are expensive, need complex sample pretreatment, consume large sample volumes, and long analysis time [ 15 , 16 ].…”

Ultrasensitive Photoelectrochemical Biosensing of Cell Surface N-Glycan Expression Based on the Enhancement of Nanogold-Assembled Mesoporous Silica Amplified by Graphene Quantum Dots and Hybridization Chain Reaction

“…28,29 As we all know, hydroxyl radical was producted from H 2 O 2 by the catalysis of the horseradish peroxidase (HRP), which further catalyzed the CL reaction of luminal with enhanced CL signal. 30,31 Thus, this reaction was applied to our strategy for offering the internal excited light source. To achieve highly sensitive detection on the PEC biosensor, enhanced photocurrent intensity and signal amplification strategies are necessary.…”

“…Therefore, chemiluminescent (CL) emission was introduced in order to avoid the external physical excitation light source inspired by the inner light source for PEC . Furthermore, to realize PEC detection, light emission with different wavelengths can be obtained by changing the experimental condition of the luminol-based CL system. , As we all know, hydroxyl radical was producted from H 2 O 2 by the catalysis of the horseradish peroxidase (HRP), which further catalyzed the CL reaction of luminal with enhanced CL signal. , Thus, this reaction was applied to our strategy for offering the internal excited light source. To achieve highly sensitive detection on the PEC biosensor, enhanced photocurrent intensity and signal amplification strategies are necessary.…”