Curcumin, a Potential Inhibitor of Up-regulation of TNF-alpha and IL-6 Induced by Palmitate in 3T3-L1 Adipocytes through NF-kappaB and JNK Pathway

Wang, Shaoling; Li, Ying; Wen, Ying; Chen, Yanfeng; Na, Li-xin; Li, Songtao; Sun, Changhao

doi:10.1016/s0895-3988(09)60019-2

Cited by 133 publications

(87 citation statements)

References 43 publications

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“…Since IL-8 or TNFa expression activated by PA can be modulated by NF-kB as reported (Joshi-Barve et al, 2007;Wang et al, 2009), we further investigated whether miR-451 expression might modulate NF-kB activation in PA-exposed HepG2 cells. To this purpose, we cotransfected with luciferase-reporter constructs containing NF-kB binding motifs and miR-451 mimics into HepG2 cells.…”

Section: Mir-451 Expression Inhibits Pa-induced Nf-äb Activationmentioning

confidence: 85%

Downregulation of microRNA-451 in non-alcoholic steatohepatitis inhibits fatty acid-induced proinflammatory cytokine production through the AMPK/AKT pathway

Hur

Lee

Kim

et al. 2015

The International Journal of Biochemistry & Cell Biology

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Mechanisms associated with the progression of non-alcoholic fatty liver disease (NAFLD) remain unclear. We attempted to identify the pattern of altered gene expression at different time points in a high fat diet (HFD)-induced NAFLD mouse model. The early up-regulated genes are mainly involved in the innate immune responses, while the late up-regulated genes represent the inflammation processes. Although recent studies have shown that microRNAs play important roles in hepatic metabolic functions, the pivotal role of microRNAs in the progression of NAFLD is not fully understood. We investigated the functions of miR-451, which was identified as a target gene in the inflammatory process in NAFLD. miR-451 expression was significantly decreased in the palmitate (PA)-exposed HepG2 cells and in liver tissues of HFD-induced non-alcoholic steatohepatitis (NASH) mice. Its decreased expressions were also observed in liver specimens of NASH patients. In vitro analysis of the effect of miR-451 on proinflammatory cytokine provided evidence for negative regulation of PA-induced interleukin (IL)-8 and tumor necrosis factor-alpha (TNF-α) production. Furthermore, miR-451 over-expression inhibited translocation of the PA-induced NF-κB p65 subunit into the nucleus. Our result showed that Cab39 is a direct target of miRNA-451 in steatotic cells. Further study showed that AMPK activated through Cab39 inhibits NF-κB transactivation induced in steatotic HepG2 cells. miR-451 over-expression in steatotic cells significantly suppressed PA-induced inflammatory cytokine. These results provide new insights into the negative regulation of miR-451 in fatty acid-induced inflammation via the AMPK/AKT pathway and demonstrate potential therapeutic applications for miR-451 in preventing the progression from simple steatosis to severely advanced liver disease.

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Section: Mir-451 Expression Inhibits Pa-induced Nf-äb Activationmentioning

confidence: 85%

Downregulation of microRNA-451 in non-alcoholic steatohepatitis inhibits fatty acid-induced proinflammatory cytokine production through the AMPK/AKT pathway

Hur

Lee

Kim

et al. 2015

The International Journal of Biochemistry & Cell Biology

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“…Highfat diet induced diabetic rats were randomly divided into 4 groups: diabetes (n Z 10, no curcumin), low-dose curcumin (n Z 10, 50 mg/kg BW), medium-dose curcumin (n Z 10, 150 mg/kg BW) and high-dose curcumin (n Z 9, 250 mg/kg BW) and were fed curcumin for 7 wk. a mechanism for the improved insulin sensitivity in fat tissue and liver [31,32]. Recently curcumin was reported to enhance insulin release by induction of b-cell electrical activity [33].…”

Section: Discussionmentioning

confidence: 99%

Curcumin improves insulin resistance in skeletal muscle of rats

Na¹,

Zhang²,

et al. 2011

Nutrition, Metabolism and Cardiovascular Diseases

163

112

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“…The anti-inflammatory effect of curcumin by PPAR-g upregulation is closely related to NF-kB pathway. 48,49 Based on the correlation, there was a significant correlation among the variables. Therefore analysis was continued with path analysis.…”

Section: Direct Effects Pmentioning

confidence: 97%

Curcumin decreased level of proinflammatory cytokines in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors NF-κB and PPAR-γ

Rahardjo

Widjajanto

Sujuti

et al. 2014

Biomarkers and Genomic Medicine

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The purpose of this study is to determine the role of curcumin in the alteration of levels of interleukin (IL)-1a, IL-6, and tumor necrosis factor a (TNF-a) as proinflammatory cytokines in monocyte culture exposed to preeclamptic plasma, as well as the effect on the transcription factors: nuclear factor kappa beta (NF-kB) and peroxisome proliferator-activated receptor g (PPAR-g). Plasma was taken from preeclamptic women (n Z 12) and normotensive pregnant women (n Z 12). Monocyte cultures were taken from nonpregnant healthy woman. Monocyte cultures were incubated with plasma for 48 hours. Curcumin in various doses were given in monocytic cultures prior to and after preeclamptic plasma exposure. The levels of IL-1a, IL-6, and TNF-a as well as NF-kB and PPAR-g in each culture were determined by enzyme-linked immunosorbent assay procedures. The final data were analyzed by analysis of variance (ANOVA) and path analysis. This study shows a significant increase (p < 0.05) in the levels of proinflammatory cytokines (IL-1a, IL-6, and TNFa) in monocyte cultures exposed (B.Rahardjo). + MODELPlease cite this article in press as: Rahardjo B, et al., Curcumin decreased level of proinflammatory cytokines in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors NF-kB and PPAR-g, Biomarkers and Genomic Medicine (2014), http://dx. Biomarkers and Genomic Medicine (2014) xx, 1e11 to preeclamptic plasma compared with normotensive pregnancy plasma. Curcumin treatment in various doses can decrease significantly (p < 0.05) proinflammatory cytokine levels in monocyte cultures that have been already stimulated by preeclamptic plasma. After curcumin treatment, there was a decreased level of nuclear NF-kB p50 and a significantly increased level of PPAR-g. Curcumin has a direct effect on decreasing the levels of nuclear NF-kB p50 and also curcumin indirectly influenced the level of nuclear NF-kB p50 by the increased level of PPAR-g. Curcumin could decrease levels of proinflammatory cytokines (IL-1a, IL-6, and TNFa) in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors, NF-kB and PPAR-g. Curcumin has potential in the prevention and future treatment of preeclampsia, through inflammation pathways assumed as being responsible for the development of preeclampsia.

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Curcumin, a Potential Inhibitor of Up-regulation of TNF-alpha and IL-6 Induced by Palmitate in 3T3-L1 Adipocytes through NF-kappaB and JNK Pathway

Cited by 133 publications

References 43 publications

Downregulation of microRNA-451 in non-alcoholic steatohepatitis inhibits fatty acid-induced proinflammatory cytokine production through the AMPK/AKT pathway

Downregulation of microRNA-451 in non-alcoholic steatohepatitis inhibits fatty acid-induced proinflammatory cytokine production through the AMPK/AKT pathway

Curcumin improves insulin resistance in skeletal muscle of rats

Curcumin decreased level of proinflammatory cytokines in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors NF-κB and PPAR-γ

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