Curcumin alters gene expression-associated DNA damage, cell cycle, cell survival and cell migration and invasion in NCI-H460 human lung cancer cells in vitro

Chiang, I-Tsang; Wang, Weishu; Liu, Hsin‐Chung; Yang, Su-Tso; Tang, Nanhong; Chung, Jing‐Gung

doi:10.3892/or.2015.4159

Cited by 36 publications

(15 citation statements)

References 36 publications

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“…Skp2 may provide sputum-based markers that have the potential to improve the early detection of LUSC ( 31 ). Skp2 can also be detected in the peripheral blood of patients with NSCLC, and has demonstrated high diagnostic value ( 32 ). These studies provide evidence that detection of Skp2 expression level should be conducted prior to surgery, assisting with the decision for surgery procedure; however, the molecular mechanism underlying regulation of Skp2 in lung cancer remains unclear and further in vitro and in vivo investigations are required.…”

Section: Discussionmentioning

confidence: 99%

Skp2 expression has different clinicopathological and prognostic implications in lung adenocarcinoma and squamous cell carcinoma

et al. 2018

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High expression of S-phase kinase associated protein 2 (Skp2) is associated with numerous clinicopathological parameters, including histology, lymph node metastasis, smoking status, differentiation and Tumor-Node-Metastasis stage in non-small cell lung cancer (NSCLC). Skp2 protein is overexpressed in lung squamous cell carcinoma (LUSC), compared with lung adenocarcinoma (LUAD), whilst the clinicopathological and prognostic implications in LUAD or LUSC remain unclear. A larger study is required to assess the differences in Skp2 expression between these NSCLC types. In the present study, the clinicopathological features and immunohistochemical expression of the Skp2 protein were studied in 500 patients with NSCLC (351 with LUAD and 149 with LUSC). Survival analyses were performed using Kaplan-Meier method and Cox regression model. Skp2 associated genes were identified based on the data from The Cancer Genome Atlas database. Skp2 was overexpressed in patients with LUSC, compared with LUAD (P<0.001). In histology subgroup analysis, differences in Skp2 protein expression were observed in patients with LUAD, based on sex, differentiation, smoking history, stage, lymph node metastasis and tumor diameter (P<0.05), but not in patients with LUSC except for smoking status. High Skp2 protein expression in patients with LUAD was associated with reduced overall survival (OS; P<0.001), but not in patients with LUSC (P=0.686). The multivariate analysis demonstrated that Skp2 expression is an independent unfavorable prognostic factor for OS in patients with LUAD (RR=1.845, P<0.05). Bioinformatics analyses revealed that minichromosome maintenance complex component 2, cell division cycle 45, replication factor C subunit 4, which are differently expressed in LUAD and LUSC, are associated with Skp2 expression and participate in DNA replication and G1/S transition. Skp2 protein expression differs in LUAD and LUSC. The clinicopathological and prognostic implications based on Skp2 expression in LUAD and LUSC should be considered different. LUSC with high Skp2 expression may have robust proliferation ability.

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Section: Discussionmentioning

confidence: 99%

Skp2 expression has different clinicopathological and prognostic implications in lung adenocarcinoma and squamous cell carcinoma

et al. 2018

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“…Examples of RNAseq studies include the effect of curcumin on a mouse model of colorectal cancer [ 83 ], parasitic mites [ 84 ], and breast cancer stem cells [ 85 ]. Microarray studies characterized the effect of curcumin on lung [ 86 , 87 ] and breast [ 88 ] cancer cell lines. Proteomic studies included studies on HCT116 colon cancer cells [ 89 ] and HepG2 cells [ 90 ].…”

Section: Discussionmentioning

confidence: 99%

Curcumin affects gene expression and reactive oxygen species via a PKA dependent mechanism in Dictyostelium discoideum

et al. 2017

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Botanicals are widely used as dietary supplements and for the prevention and treatment of disease. Despite a long history of use, there is generally little evidence supporting the efficacy and safety of these preparations. Curcumin has been used to treat a myriad of human diseases and is widely advertised and marketed for its ability to improve health, but there is no clear understanding how curcumin interacts with cells and affects cell physiology. D. discoideum is a simple eukaryotic lead system that allows both tractable genetic and biochemical studies. The studies reported here show novel effects of curcumin on cell proliferation and physiology, and a pleiotropic effect on gene transcription. Transcriptome analysis showed that the effect is two-phased with an early transient effect on the transcription of approximately 5% of the genome, and demonstrates that cells respond to curcumin through a variety of previously unknown molecular pathways. This is followed by later unique transcriptional changes and a protein kinase A dependent decrease in catalase A and three superoxide dismutase enzymes. Although this results in an increase in reactive oxygen species (ROS; superoxide and H2O2), the effects of curcumin on transcription do not appear to be the direct result of oxidation. This study opens the door to future explorations of the effect of curcumin on cell physiology.

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“…Curcumin (0.1–3 mg/kg body weight) was shown to suppress telomerase reverse transcriptase enzyme [55] and reduce Bcl-2 expression [56]. Curcumin (2 µM) interacts with various proteins involved in angiogenesis, metastasis, and cell survival, as well as interferes with dysregulated signaling pathways in cancer cells, for instance phosphoinositide 3-kinase (PI3K)/Akt and NF-κB [57]. Curcumin exerts numerous effects by targeting several molecular and cellular pathways such as cell death, p53, Akt, mitogen-activating protein kinases (MAPK), microRNAs, and PTEN [45,58].…”

Section: Anticarcinogenic Potency and Molecular Mechanisms Inducedmentioning

confidence: 99%

Curcumin Combination Chemotherapy: The Implication and Efficacy in Cancer

2019

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Many chemotherapeutic drugs have been used for the treatment of cancer, for instance, doxorubicin, irinotecan, 5-fluorouracil, cisplatin, and paclitaxel. However, the effectiveness of chemotherapy is limited in cancer therapy due to drug resistance, therapeutic selectivity, and undesirable side effects. The combination of therapies with natural compounds is likely to increase the effectiveness of drug treatment as well as reduce the adverse outcomes. Curcumin, a polyphenolic isolated from Curcuma longa, belongs to the rhizome of Zingiberaceae plants. Studies from in vitro and in vivo revealed that curcumin exerts many pharmacological activities with less toxic effects. The biological mechanisms underlying the anticancer activity of co-treatment curcumin and chemotherapy are complex and worth to discuss further. Therefore, this review aimed to address the molecular mechanisms of combined curcumin and chemotherapy in the treatment of cancer. The anticancer activity of combined nanoformulation of curcumin and chemotherapy was also discussed in this study. Taken together, a better understanding of the implication and underlying mechanisms of action of combined curcumin and chemotherapy may provide a useful approach to combat cancer diseases.

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Curcumin alters gene expression-associated DNA damage, cell cycle, cell survival and cell migration and invasion in NCI-H460 human lung cancer cells in vitro

Cited by 36 publications

References 36 publications

Skp2 expression has different clinicopathological and prognostic implications in lung adenocarcinoma and squamous cell carcinoma

Skp2 expression has different clinicopathological and prognostic implications in lung adenocarcinoma and squamous cell carcinoma

Curcumin affects gene expression and reactive oxygen species via a PKA dependent mechanism in Dictyostelium discoideum

Curcumin Combination Chemotherapy: The Implication and Efficacy in Cancer

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