Curcumin Ameliorates Copper-Induced Neurotoxicity Through Inhibiting Oxidative Stress and Mitochondrial Apoptosis in SH-SY5Y Cells

Xiang, Biao; Li, Daowen; Chen, Yiqiang; Li, Meng; Zhang, Yuan; Sun, Tun; Tang, Shusheng

doi:10.1007/s11064-020-03173-1

Cited by 28 publications

(8 citation statements)

References 48 publications

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“…In this study, 10 µM CUR significantly reduced the accumulation of ROS induced by LPS in MAC-T cells ( Figure 2 A,B). This effect is consistent with the research results of Xiang and Biao, et al [ 30 ]. In addition, CUR significantly reduced the increase in MDA content in cells induced by LPS ( Figure 2 C).…”

Section: Discussionsupporting

confidence: 94%

Curcumin Alleviates LPS-Induced Oxidative Stress, Inflammation and Apoptosis in Bovine Mammary Epithelial Cells via the NFE2L2 Signaling Pathway

Fang

Shen

et al. 2021

Toxins

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Lipopolysaccharide (LPS) is an endotoxin, which may cause immune response and inflammation of bovine mammary glands. Mastitis impairs animal health and results in economic loss. Curcumin (CUR) is a naturally occurring diketone compound, which has attracted widespread attention as a potential anti-inflammatory antioxidant. The purpose of this study is to investigate whether CUR can reduce the damage of bovine mammary epithelial cells (MAC-T) induced by LPS and its underlying molecular mechanism. The MAC-T cell line was treated with different concentrations of LPS and CUR for 24 h. The results showed that CUR rescued the decrease of MAC-T cell viability and cell damage induced by LPS. At the same time, 10 µM CUR and 100 µg/mL LPS were used to treat the cells in the follow-up study. The results showed CUR treatment reduced the accumulation of reactive oxygen species (ROS), the expression of inflammatory cytokines (tumor necrosis factor-a (TNF-α), interleukin-8 (IL-8), IL-6 and IL-1β) and the rate of apoptosis induced by LPS. These effects were associated with the activation of the nuclear factor E2-related factor 2 (NFE2L2)-antioxidant response element (ARE) pathway coupled with inactivation of the nuclear factor-κB (NF-κB) inflammatory and caspase/Bcl2 apoptotic pathways.

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Section: Discussionsupporting

confidence: 94%

Curcumin Alleviates LPS-Induced Oxidative Stress, Inflammation and Apoptosis in Bovine Mammary Epithelial Cells via the NFE2L2 Signaling Pathway

Fang

Shen

et al. 2021

Toxins

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“…In an in vitro experimental model of neurodegenerative disease, Armagan and Naziroglu [11] reported the neuroprotective action of curcumin by modulating oxidative stress. In addition, this polyphenol is capable to decrease neurotoxicity, which is proven by other models of neurological disorders in vitro [11][12][13][14][15] and in vivo studies [16,17].…”

Section: Introductionmentioning

confidence: 89%

Impact of Free Curcumin and Curcumin Nanocapsules on Viability and Oxidative Status of Neural Cell Lines

Bissacotti

Copetti

Gündel

et al. 2021

Preprint

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Curcumin is an active polyphenol substance found in the highest concentrations in the roots of Curcuma longa. Its health benefits have led to recent increases in the consumption of curcumin. It has anti-inflammatory and antioxidant activities and is a potent neuroprotective against diseases of the brain. Nevertheless, its low bioavailability and its relative difficulty crossing the blood-brain barrier limit curcumin’s use for these purposes. Curcumin-loaded nanoparticles may be an effective treatment for several diseases although there is a paucity of studies reporting its safety in the central nervous system (CNS). Therefore, this study aimed to identify non-neurotoxic concentrations of free curcumin and two nanoformulations of curcumin. Cell lines BV-2 and SH-SY5Y, both originating from the CNS, were evaluated after 24, 48, and 72 h of treatment with free curcumin and nanocapsules We measured viability, proliferation and dsDNA levels. We measured levels of reactive oxygen species and nitric oxide as proxies for oxidative stress in culture supernatants. We found that free curcumin was toxic at 10 and 20 µM, principally at 72 h. Nanoformulations were more neurotoxic than the free form. Safe concentrations of free curcumin are between 1-5 µM, and these concentrations were lower for nanoformulations. We determined the ideal concentrations of free curcumin and nanocapsules serving as a basis for studies of injuries that affect the CNS.

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“…Again, the haem protein was detectable in the cell nucleus prior to caspase cascade activation or apoptosis induction [32]. Recently, nuclear translocation of C c induced by copper has been found in neuroblastoma cells [33]. Such outcomes hint to novel functions for nuclear C c , beyond the well‐known roles in the cytosol and mitochondria.…”

Section: Mitochondrial Cytochrome C As a Signalling Factor Of Dna Lesions In The Nucleusmentioning

confidence: 99%

Mitochondrial cytochrome c shot towards histone chaperone condensates in the nucleus

et al. 2021

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Despite mitochondria being key for the control of cell homeostasis and fate, their role in DNA damage response (DDR) is usually just regarded as an apoptotic trigger. However, growing evidence points to mitochondrial factors modulating nuclear functions. Remarkably, after DNA damage, cytochrome c (Cc) interacts in the cell nucleus with a variety of well-known histone chaperones, whose activity is competitively inhibited by the hemeprotein. As nuclear Cc inhibits the nucleosome assembly/disassembly activity of histone chaperones, it might indeed affect chromatin dynamics and histone deposition on DNA. Several histone chaperones actually interact with Cc Lys residues through their acidic regions, which are also involved in heterotypic interactions leading to liquid-liquid phase transitions responsible for the assembly of nuclear condensates, including heterochromatin. This relies on dynamic histone-DNA interactions that can be modulated by acetylation of specific histone Lys residues.Thus, Cc may have a major regulatory role in DNA repair by fine-tuning nucleosome assembly activity and likely nuclear condensate formation.

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Curcumin Ameliorates Copper-Induced Neurotoxicity Through Inhibiting Oxidative Stress and Mitochondrial Apoptosis in SH-SY5Y Cells

Cited by 28 publications

References 48 publications

Curcumin Alleviates LPS-Induced Oxidative Stress, Inflammation and Apoptosis in Bovine Mammary Epithelial Cells via the NFE2L2 Signaling Pathway

Curcumin Alleviates LPS-Induced Oxidative Stress, Inflammation and Apoptosis in Bovine Mammary Epithelial Cells via the NFE2L2 Signaling Pathway

Impact of Free Curcumin and Curcumin Nanocapsules on Viability and Oxidative Status of Neural Cell Lines

Mitochondrial cytochrome c shot towards histone chaperone condensates in the nucleus

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