Curcumin and Paclitaxel Co-loaded Heparin and Poloxamer P403 Hybrid Nanocarrier for Improved Synergistic Efficacy in Breast Cancer

Abstract

Introduction: Multi-drug nanosystem has been employed in several therapeutic models due to the synergistic effect of the drugs and/or bioactive compounds, which help in tumor-targeting and limit usual side effects of chemotherapy. Methods: In this research, we developed the amphiphilic Heparin-Poloxamer P403 (HSP) nanogel that can load curcumin (CUR) and Paclitaxel (PTX) through the hydrophobic core of Poloxamer P403. The features of HSP nanogel are assessed through Fourier-transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), differential light scattering (DLS), and critical micelle concentration (CMC). Nanogel and its duel-loaded platform show high stability and spherical morphology. Results: The drug release profile indicates fast release at pH 5.5, suggesting effective drug distribution at the tumor site. In vitro research confirms lower cytotoxicity of HSP@CUR@PTX compared with free PTX and higher inhibition effect with MCF-7 than HSP@PTX. These results support the synergism between PTX and CUR. Conclusion,: HSP@CUR@PTX suggests a prominent strategy for achieving the synergistic effect of PTX and CUR to circumvent undesirable effects in breast cancer treatment.

Synthesis of heparin-and-poloxamer P407-based nanogel carrying PTX for orientating in treatment of breast cancer

“…Determination of PTX content in HR-P407/ PTX was carried out using a UV-vis Agilent 8453 spectrometer (Agilent, USA) at 254 nm wavelength. The percentage of drug loading capacity (DL%) and encapsulation efficiency (EE%) of the nanogel were calculated according to the following formulas [9,[18][19][20] HR-P407/PTX samples were dissolved in 1 ml DI water, and the PTX control sample was dissolved in 1 ml ethanol. The solutions were then placed in each dialysis bag (MWCO 3500 Da) and immersed in 10 ml PBS buffer at pH 7.4 or 5.5, respectively.…”

Fucoidan and dendrimer-based nanocapsule exhibiting effectiveness in methotrexate controlled delivery towards rheumatoid arthritis treatment

“…To determine the release mechanism of MTX from the G3.0-Fu system, the release data were compared with four mathematical models of order 0, first order, Higuchi and Korsmeyer. The mean dissolution time (MDT) that was used to evaluate the G3.0-Fu system time between MTX and MDT was calculated using the formula (1) [16,17].…”

“…Nano-drug delivery systems play crucial roles in improving the effectiveness of treatment and reducing side effects of hydrophobic drugs. They enhance drug solubility, increase drug exposure and absorption at the target site, and control drug release rates to maintain stable drug concentrations in the body [12][13][14][15][16][17][18]. Polyamidoamine (PAMAM) dendrimer is a nano-sized and hyper-branched polymer.…”

Construction of Curcumin and Paclitaxel Co-Loaded Lipid Nano Platform and Evaluation of Its Anti-Hepatoma Activity in vitro and Pharmacokinetics in vivo

“…Numerous groups have attempted to develop nanodrug delivery systems, including micelles, polymeric nanoparticles, liposomes and solid lipid nanoparticles, in an effort to enhance the therapeutic efficacy of CU-PTX combinations and address the drug delivery issues. [23][24][25][26][27] Due to their exceptional biocompatibility, stability, safety, and modifiability, lipid nanoparticles have been researched as carriers for delivering insoluble medications like CU and/or PTX. [28][29][30] Particularly, Curcumin-human serum albumin nanoparticles decorated with programmed death ligand 1 (PDL1) binding peptide not only improve tumor-targeting drug delivery but also reestablish immune response by blocking PDL1/PD1 interaction.…”