Curcumin attenuates heat-stress-induced oxidant damage by simultaneous activation of GSH-related antioxidant enzymes and Nrf2-mediated phase II detoxifying enzyme systems in broiler chickens

Zhang, J F; Bai, Kaiwen; Su, W.P. Daniel; Wang, A A; Zhang, L L; Huang, Kehe; Wang, T

doi:10.3382/ps/pex408

Cited by 99 publications

(76 citation statements)

References 39 publications

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“…Glutathione antioxidant system plays a pivotal role in cellular defense against ROS, GSH-Px function either directly or indirectly as antioxidants, and GSH plays an important role in scavenging reactive oxygen species [47]. It has been reported that dietary chitosan supplementation could enhance the serum SOD, CAT, and GSH-Px of diquat challenged weaning pigs [48]. In addition, dietary chitosan supplementation can prevent lipid peroxidation induced by LPS and retain GSH and CAT activity [42].…”

Section: Discussionmentioning

confidence: 99%

Chitosan Oligosaccharides Protect Sprague Dawley Rats from Cyclic Heat Stress by Attenuation of Oxidative and Inflammation Stress

Lan

Chang

et al. 2019

Animals

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Chitosan oligosaccharides (COS) exhibits antioxidant and anti-inflammatory properties. The aim of this study was to evaluate the effects of COS on antioxidant system and inflammatory response in heat-stressed rats. A total of 30 male rats were randomly divided to three groups and reared at either 24 °C or 35 °C for 4 h/d for this 7-day experiment: CON, control group with basal diet; HS, heat stress group with basal diet; HSC, heat stress with 200mg/kg COS supplementation. Compared with the CON group, HS significantly decreased (p < 0.05) average daily gain (ADG); average daily feed intake (ADFI); the relative weight of spleen and kidney; the level of liver CAT, GSH-Px, T-AOC, and IL-10; spleen SOD, GSH-Px, GSH, and IL-10; and kidney SOD, GSH-Px, T-AOC, and IL-10, while significantly increased the MDA concentration in liver, spleen, and kidney; the liver IL-1β concentration; and spleen and kidney IL-6 and TNF-α concentration. In addition, dietary COS supplementation significantly improved (p < 0.05) ADG; the relative weight of spleen and kidney; the level of liver GSH-Px, spleen GSH-Px, GSH, and IL-10; and kidney GSH-Px, while significantly decreased (p < 0.05) liver IL-1β concentration under heat stress condition. Collectively, COS was beneficial to heat-stressed rats by alleviating oxidative damage and inflammatory response.

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Section: Discussionmentioning

confidence: 99%

Chitosan Oligosaccharides Protect Sprague Dawley Rats from Cyclic Heat Stress by Attenuation of Oxidative and Inflammation Stress

Lan

Chang

et al. 2019

Animals

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“…Previous studies have confirmed that various feed supplements, such as curcumin, lycopene, and some microelement can alleviate oxidative damage caused by chronic heat stress in broilers by modulating the expression of Nrf2 ( Sahin et al., 2016 , 2017 ; Zhang et al., 2018 ). However, under the condition of acute heat stress, limited information is available about the antioxidant potential and related regulatory mechanism of broilers.…”

Section: Introductionmentioning

confidence: 91%

Effects of acute heat stress at different ambient temperature on hepatic redox status in broilers

Miao

Xie

et al. 2020

Poultry Science

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This study aimed to investigate the effects of different acute high ambient temperatures on redox status in liver of broilers. A total of 144 35-day-old Arbor Acres broilers were randomly divided into 4 groups with 6 replicates of 6 birds each and subsequently distributed in different environment chambers for acute heat stress. The temperature of 4 environment chambers were set to 26°C (control), 29°C, 32°C, 35°C for 6 h, respectively. Various indicators were tested to evaluate hepatic redox status. Then, the hallmarks of hepatocellular antioxidant and apoptosis were measured by qRT-PCR and Western Blot. The results showed that with the ambient temperature increase (i) the content of hydrogen peroxide ( H 2 O 2 ) and protein carbonyl ( PC ) in the liver of broilers increased significantly ( P < 0.05), but the content of malondialdehyde ( MDA ) and 8-hydroxyguanosine ( 8-OHdG ) was not affected; (ii) the activity of catalase ( CAT ) and glutathione reductase ( GR ) increased significantly ( P < 0.05). Similarly, the superoxide dismutase ( SOD ) had an increasing tendency ( P = 0.07), and the content of the reduced glutathione ( GSH ) was also significantly increased ( P < 0.05) under high temperature; (iii) the heat shock protein ( HSP70 ), nuclear factor erythroid-2-related factor 2 ( Nrf2 ), and other antioxidant gene ( HO-1 , NQO1 , GCLc , GST , SOD1 , SOD2 , CAT , Prx3 ) were upregulated in broilers liver. Moreover, the protein level of HSP70, Nrf2, and Prx3 were also upregulated; (iv) high temperature upregulated the antiapoptotic gene expression ( BCL-2 ); however, the proapoptotic genes ( BAK1 , caspase-3 , and caspase-9 ) did not change significantly; meanwhile, there was no significant changes in the protein level of caspase-3 and caspase-9. The results of this study indicated that 35-day-old Arbor Acres broilers have a certain tolerance to oxidative stress induced by high ambient temperature. Six hours of acute heat stress–activated Nrf2 signaling pathway. Meanwhile, the expression of related antioxidant genes and proteins is upregulated, consequently resulted in increased antioxidant enzymes activity and GSH. These effects enable the body to scavenge large amounts of reactive oxygen species produced by high temperature and prevent the occurrence of apoptosis.

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“…The protective effects of other phytochemicals against Nrf2-related AO enzyme changes due to the presence of HS were also reported. For example, curcumin supplementation (50-200 mg/kg) was shown to improve AO defences of HS-exposed broilers, as evidenced by increasing the GSH content and GSH-related enzyme activities and inducing the expression of Nrf2 and Nrf2-mediated phase II detoxifying enzyme genes [259]. Interestingly, birds in the HS group showed increased MDA, protein carbonyl (PC), 8-hydroxydeoxyguanosine (8-OHdG) and some apoptosis markers, including caspase-3 and caspase-9 mRNA levels and activity.…”

Section: Heat Stressmentioning

confidence: 99%

Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update

et al. 2019

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Poultry in commercial settings are exposed to a range of stressors. A growing body of information clearly indicates that excess ROS/RNS production and oxidative stress are major detrimental consequences of the most common commercial stressors in poultry production. During evolution, antioxidant defence systems were developed in poultry to survive in an oxygenated atmosphere. They include a complex network of internally synthesised (e.g., antioxidant enzymes, (glutathione) GSH, (coenzyme Q) CoQ) and externally supplied (vitamin E, carotenoids, etc.) antioxidants. In fact, all antioxidants in the body work cooperatively as a team to maintain optimal redox balance in the cell/body. This balance is a key element in providing the necessary conditions for cell signalling, a vital process for regulation of the expression of various genes, stress adaptation and homeostasis maintenance in the body. Since ROS/RNS are considered to be important signalling molecules, their concentration is strictly regulated by the antioxidant defence network in conjunction with various transcription factors and vitagenes. In fact, activation of vitagenes via such transcription factors as Nrf2 leads to an additional synthesis of an array of protective molecules which can deal with increased ROS/RNS production. Therefore, it is a challenging task to develop a system of optimal antioxidant supplementation to help growing/productive birds maintain effective antioxidant defences and redox balance in the body. On the one hand, antioxidants, such as vitamin E, or minerals (e.g., Se, Mn, Cu and Zn) are a compulsory part of the commercial pre-mixes for poultry, and, in most cases, are adequate to meet the physiological requirements in these elements. On the other hand, due to the aforementioned commercially relevant stressors, there is a need for additional support for the antioxidant system in poultry. This new direction in improving antioxidant defences for poultry in stress conditions is related to an opportunity to activate a range of vitagenes (via Nrf2-related mechanisms: superoxide dismutase, SOD; heme oxygenase-1, HO-1; GSH and thioredoxin, or other mechanisms: Heat shock protein (HSP)/heat shock factor (HSP), sirtuins, etc.) to maximise internal AO protection and redox balance maintenance. Therefore, the development of vitagene-regulating nutritional supplements is on the agenda of many commercial companies worldwide.

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Curcumin attenuates heat-stress-induced oxidant damage by simultaneous activation of GSH-related antioxidant enzymes and Nrf2-mediated phase II detoxifying enzyme systems in broiler chickens

Cited by 99 publications

References 39 publications

Chitosan Oligosaccharides Protect Sprague Dawley Rats from Cyclic Heat Stress by Attenuation of Oxidative and Inflammation Stress

Chitosan Oligosaccharides Protect Sprague Dawley Rats from Cyclic Heat Stress by Attenuation of Oxidative and Inflammation Stress

Effects of acute heat stress at different ambient temperature on hepatic redox status in broilers

Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update

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