Curcumin inhibits bladder cancer progression via regulation of β-catenin expression

Abstract

Bladder cancer has a considerable morbidity and mortality impact with particularly poor prognosis. Curcumin has been recently noticed as a polyphenolic compound separated from turmeric to regulate tumor progression. However, the precise molecular mechanism by which curcumin inhibits the invasion and metastasis of bladder cancer cells is not fully elucidated. In this study, we investigate the effect of curcumin on the bladder cancer as well as possible mechanisms of curcumin. The expression of β-catenin was detected by quantitative real-time polymerase chain reaction and immunohistochemical analysis in a series of bladder cancer tissues. In addition, bladder cancer cell lines T24 and 5637 cells were treated with different concentrations of curcumin. The cytotoxic effect of curcumin on cell proliferation of T24 and 5637 cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The migration and invasion capacity of T24 and 5637 cells were measured by transwell assay. The effects of curcumin on expression levels of β-catenin and epithelial-mesenchymal transition marker were determined by western blotting. The β-catenin expression was significantly upregulated in bladder cancer tissues when compared with corresponding peri-tumor tissues. Furthermore, curcumin inhibited the cell proliferation of T24 and 5637 cells, and curcumin reduced the migration and invasive ability of T24 and 5637 cells via regulating β-catenin expression and reversing epithelial-mesenchymal transition. Curcumin may be a new drug for bladder cancer.

Cordycepin induces apoptosis in human bladder cancer T24 cells through ROS-dependent inhibition of the PI3K/Akt signaling pathway

“…The common treatment for bladder cancer focuses on radical cystectomy, but most patients experience relapse after excision (14). Because of this, adjuvant chemotherapy is usually performed in an effort to delay recurrence and prolong survival (14,15). However, there are reported that adjuvant chemotherapy responded to 50% of muscle-invasive bladder cancer patients (15,16).…”

“…Because of this, adjuvant chemotherapy is usually performed in an effort to delay recurrence and prolong survival (14,15). However, there are reported that adjuvant chemotherapy responded to 50% of muscle-invasive bladder cancer patients (15,16). Therefore, there is a need to develop a novel treatment strategies for the overcome these challenges of bladder cancer.…”

Cytotoxicity of Standardized Curcuminoids Mixture against Epithelial Ovarian Cancer Cell Line SKOV-3

“…Several studies have confirmed the ability of curcuminoid members to suppress cancer cell migration and invasion. For example, curcumin inhibits migrated cells from entering into the wound area of lung [28], breast [29], and bladder [30] cancer cells, while DMC inhibits the motility of breast [31] and prostate [32] cancer cells. Further, BDMC inhibits the migration of ovarian [33] and lung [34] cancer cells.…”

“…While curcuminoids are composed of three main members-curcumin, DMC, and BDMC-all have shown efficient anticancer effects against various malignancies and induce apoptosis, but with different potencies. Although many studies present curcumin as the active ingredient in turmeric, with a strong ability to kill different cancer cells such as hepatic [37], lung [28], breast [38], pancreas [27], bladder [30], and ovarian [39] cancer cells, DMC, which is more stable than curcumin, was reported by Simon et al [40] to exhibit the strongest antitumor activity among the three curcuminoids against breast cancer cells. DMC has also shown ability to inhibit the growth of prostate cancer cells [32].…”

Curcumin inhibits the development of non‑small cell lung cancer by inhibiting autophagy and apoptosis

“…The present study focused on curcumin, as a natural polyphenol isolated from the spice turmeric (26). Previous studies have demonstrated that curcumin suppresses cytotoxicity and DNA injury by suppressing oxidative damage caused by reactive oxygen species (27,28).…”