Curcumin inhibits hypoxia-induced migration in K1 papillary thyroid cancer cells

Abstract

Curcumin, traditionally used as food and medicinal purposes, has recently been reported to have protective efficacy against hypoxia. Hypoxia is one of the important reactive factors in tumor metastasis, which is a key problem in clinical thyroid cancer therapy. In present study, we investigate the anti-metastatic effect of curcumin on the K1 papillary thyroid cancer cells as well as its potential mechanisms. The results show that curcumin effectively inhibits hypoxia-induced reactive oxygen species (ROS) upregulation and significantly decreases the mRNA and protein expression levels of hypoxia-inducible factor-1a (HIF-1a) in K1 cells. Curcumin also decreases the DNA binding ability of HIF-1a to hypoxia response element (HRE). Furthermore, curcumin enhances E-cadherin expression, inhibits metalloproteinase-9 (MMP-9) enzyme activity, and weakens K1 cells migration under hypoxic conditions. In summary, these results indicate that curcumin possesses a potent anti-metastatic effect and might be an effective tumoristatic agent for the treatment of aggressive papillary thyroid cancers.Keywords: Curcumin, K1 papillary thyroid cancer cell, hypoxia-inducible factor-1a, metastasis Experimental Biology and Medicine 2015; 240: 925-935.

Retracted Article: Down-regulation of Rab10 inhibits hypoxia-induced invasion and EMT in thyroid cancer cells by targeting HIF-1α through the PI3K/Akt pathway

“…HIF-1a, a signal transcription factor, is a hallmark of cell invasion and a crucial regulator of the EMT process. 26,27,43 HIF-1a could be induced upon hypoxia, which will inuence the expression and activity of some important transcription factors and subsequently cause inhibition of E-cadherin in cancer cells. [27][28][29] In this study, we found that Rab10 down-regulation decreased hypoxia-induced HIF-1a expression in thyroid cancer cells; HIF-1a knockdown inhibited hypoxia-induced decrease in E-cadherin expression and the inhibitory effect was enhanced by Rab10 down-regulation.…”

Induction of ROS-independent DNA damage by curcumin leads to G2/M cell cycle arrest and apoptosis in human papillary thyroid carcinoma BCPAP cells

“…Curcumin [bis(4-hydroxy-3methoxy-phenyl)-1,6-heptadiene-3,5-dione] is a type of natural dietary polyphenolic compound isolated as a yellow pigment from turmeric (Curcuma longa), which is extensively used in Indian and Chinese medicine for the management of various diseases. 6 Recent studies from our group have also proved that curcumin inhibits the invasion and metastasis of K1 cells under both normoxic 7,8 and hypoxic conditions 9 via modulating E-cadherin and matrix metalloproteinase-9 expressions. 5 In our previous study, we reported that curcumin induces apoptosisdependent cell death in K1 papillary thyroid cancer cells.…”

Curcumin inhibits hypoxia-induced proliferation and invasion of MG-63 osteosarcoma cells via downregulating Notch1

“…Hypoxia, caused by a limited blood supply from aberrant neovascularization and a rapidly growing tumor mass, is a common characteristic of solid tumors (3). Hypoxic conditions are hypothesized to facilitate tumor progression by activating signaling pathways involved in cell proliferation, angiogenesis, apoptosis, invasion and metastasis (4). The transcription factor hypoxia-inducible factor-1α (HIF-1α) is a key element in the cellular response to hypoxia and is widely expressed in a variety of solid tumors, including osteosarcoma (5).…”

“…The antitumor effect of curcumin is of increasing interest (16)(17)(18)(19). It has been reported that curcumin may exert antitumor effects in normoxic and hypoxic conditions (4). As hypoxia is a key characteristic of the tumor microenvironment in osteosarcoma, the identification of molecules that contribute to the antitumor effects of curcumin may provide potential therapeutic targets.…”